魚類の塩類細胞におけるイオンのベクトル輸送機構モデルの確立

鱼盐细胞中离子载体传输机制模型的建立

• 批准号: 17770055
• 负责人: 廣井 準也
• 金额: $ 2.37万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17770055/
• 关键词: 塩類細胞; イオン輸送体; 環境適応; 淡水; 海水; ティラピア

1.前年度に研究代表者は,従来の知見では魚類の海水型イオン排出性塩類細胞にのみ発現するとされていたcation chloride cotransporter (CCC)が淡水型イオン吸収性塩類細胞にも発現し機能していることを示唆するデータを得た.本年度は,まずティラピアの鰓から4種類のCCCアイソフォームをクローニングした.次に,real-time PCRによってmRNAのtissue distributionと淡水-海水移行時の継時変化を測定したところ,淡水および海水の鰓・卵黄嚢上皮で強く発現するCCCがそれぞれ1種類ずつ存在することが明らかになった.さらに,この2種類のCCCを特異的に認識できる抗体を作成し免疫染色を行ったところ,2種類のCCCは同一細胞に局在せず,淡水型塩類細胞のapical membraneおよび海水型塩類細胞のbasolateral membraneにそれぞれ特異的に局在することが明らかとなった.これらの結果は,これまで混迷を極めていた塩類細胞のイオン吸収機構研究に解明の糸口を与えるものである.2.その生態的特徴から海水耐性が異なると予測されるサケ科魚類3種,レイクトラウト,ブルックトラウトおよび大西洋サケについて,海水耐性と鰓のイオン輸送体の局在様式等を比較したところ,レイクトラウトとブルックトラウトはある程度の海水適応力を備えているものの,淡水海水移行時における塩類細胞の分布様式の変化が大西洋サケと大きく異なることが明らかとなった.レイクトラウトとブルックトラウトはサケ科の中で最も原始的とされるSalvelinus属に属しているため,これら2種の海水適応様式はサケ科魚類の祖先種が備えていたものと相同である可能性が考えられる.この成果をJ Exp Biol誌上で公表した.

1. In the previous year, the representative of the research team came to know that there was a significant increase in the number of exhaust cells in the sea water type, such as the sea water type, the cation chloride cotransporter (CCC) type, the fresh water type, the absorption type, the fresh water type, This year, there will be a general election for the first time this year. This year, there will be an increase in the number of CCC events in this year. In the second place, real-time PCR has been used to determine the temperature of fresh water-sea water during the migration of fresh water and sea water, and the yolk epithelium of fresh water and sea water has been detected. There are significant changes in the expression of CCC protein 1 in fresh water. The anti-CCC antibodies of class 2 and type 2 were detected by immunostaining, and the same type of CCC was detected by immunostaining, while that of the cell type of freshwater type, type of apical membrane, type of sea water, type of basolateral membrane, type of sea water, type of sea water, type of basolateral membrane, type of sea water, type of cell, type of According to the results of the experiment, many fans have been involved in the study of the absorption mechanism to understand the difference between the mouth and the mouth. 2. There are three kinds of special seawater endurance products in the department of marine science and technology, which are used in the Atlantic Ocean and the Atlantic Ocean. The sea water endurance capacity is higher than that in the Atlantic Ocean, and the sea water endurance capacity is higher than that in the Atlantic. During the migration of fresh water and sea water, the distribution of atmospheric cells in the Atlantic Ocean is in the form of environmental protection. The most primitive species in the family, the genus Salvelinus, is the most primitive species in the family. This is the most primitive genus in the family, and the most primitive genus in the family is the most primitive in the family. The results are listed in the public table on the J Exp Biol log.

项目成果

Functional classification of chloride cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na^+/K^+-ATPase, Na^+/K^+/2Cl^- cotransporter and CFTR anion channel

通过 Na^ /K^ -ATP 酶、Na^ /K^ /2Cl^- 协同转运蛋白和 CFTR 阴离子通道三重免疫荧光染色，对广盐莫桑比克罗非鱼 (Oreochromis mossambicus) 胚胎中氯离子细胞进行功能分类

• 发表时间: 2005
• 期刊: The Journal of Experimental Biology 208(11)
• 作者: Kawaguchi;M. et al.;Junya Hiroi et al.
• 通讯作者: Junya Hiroi et al.

Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei

• DOI: 10.1016/j.gene.2006.11.012
• 发表时间: 2007-05-01
• 期刊: GENE
• 影响因子: 3.5
• 作者: Kawaguchi, Marl;Yasumasu, Shigeki;Iuchi, Ichiro
• 通讯作者: Iuchi, Ichiro

Purification and gene cloning of Fundulus heteroclitus hatching enzyme. A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts, Fundulus heteroclitus and medaka Oryzias latipes

异斜眼底孵化酶的纯化及基因克隆。

• 发表时间: 2005
• 期刊: The FEBS Journal 272(17)
• 作者: Kawaguchi;M. et al.;Junya Hiroi et al.;Junya Hiroi et al.;Mari Kawaguchi et al.
• 通讯作者: Mari Kawaguchi et al.

Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar

• DOI: 10.1242/jeb.002030
• 发表时间: 2007-03-15
• 期刊: JOURNAL OF EXPERIMENTAL BIOLOGY
• 影响因子: 2.8
• 作者: Hiroi, Junya;McCormick, Stephen D.
• 通讯作者: McCormick, Stephen D.

Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater.

从莫桑比克罗非鱼胚胎中分离并在淡水和海水中孵育的卵黄囊制剂（卵黄球）的氯周转和离子传输活性。

• 发表时间: 2005
• 期刊: J Exp Biol, 208
• 作者: Hiroi J;et al.
• 通讯作者: et al.