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Degradation of Corrosion Resistance of Implant Metals in Biological effects with Adhesion Cells

粘附细胞生物效应中植入金属耐腐蚀性的降低

基本信息

批准号：
18500360
负责人：
MORITA Masafumi
金额：
$ 2.52万
依托单位：
Saitama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500360/
关键词：
Corrosion resistance Biocompatibility Electro Chemical Stability Dissolved Oxygen Medical Device Titanium Alloy Biomaterials

项目摘要

In order to maintain biocompatibility inside the human body, metallic implants must possess high corrosion resistance. The purpose of this work was to investigate the mechanism behind the release of metal ions from implant materials into the surrounding tissue. In this study, the authors examined the biological effects (based on (a) adhesion of cells and (b) cell metabolic activity) of biofilms formed by L929 fibroblastic cells and U937 macrophages on the corrosion resistance of a pure nickel plate. Pure Ni is not actually used as a biomaterial, but Ni, which corrodes easily, was used to investigate variations in metal ion release under different experimental conditions, and to clarify the corrosion mechanism.We studied on the affect of adhesive cells on the corrosion resistance of implant metal. L929 fibroblastic cells, which were adhered on the surface of the metals, were cultured for 3 days. Then, the concentration of Ni ion dissolved to the cultured medium was measured with the ICP … More analyzer.About pure Ni, the amount of Ni ion concentration was increased with the cell density. For NiTi alloy, the amount of dissolution of Ni ion was slighter than that of pure Ni, but the correlation of the concentration between Ni ion and the cell density was significant. On the other hand, when the protein of the cell was fixed with 10% formaldehyde solution, the dissolved Ni ion concentration was slightly compared with that of the living cells. As the results, it was suggested that the corrosion resistance of implant metal was closely depended on the existence of the living adhesive cells. The following conclusions have been reached in this study on the effect of metallic corrosion with cell. (1) The corrosion rate of metallic implant in vitro, the presence of the cell adhesion is controlled. An anchorage-dependent cell (L929) was promoted the release of metallic ions. (2) The metabolic activity of the cell influenced metallic corrosion. The cells that were lost the biological activities did not promote the metal corrosion even if the cells were adhered. Cell adhesion molecules (CAMs) such as fibronectin hardly promoted the release of metallic ions. Less

为了保持人体内的生物相容性，金属植入物必须具有高的耐腐蚀性。这项工作的目的是研究金属离子从植入材料释放到周围组织的机制。在这项研究中，作者检查了L929成纤维细胞和U937巨噬细胞形成的生物膜对纯镍板耐腐蚀性的生物学效应（基于（a）细胞粘附和（B）细胞代谢活性）。纯镍实际上并不被用作生物材料，但镍是一种易腐蚀的金属，为了研究在不同实验条件下金属离子释放的变化，并阐明腐蚀机理，我们研究了粘附细胞对植入金属耐腐蚀性的影响。将粘附在金属表面上的L929成纤维细胞培养3天。然后，用ICP测量溶解到培养基中的Ni离子的浓度 ...更多信息对于纯Ni，Ni离子浓度的量随着电池密度而增加。对于NiTi合金，Ni离子的溶解量比纯Ni的溶解量小，但Ni离子的浓度与晶胞密度有显著的相关性。另一方面，当用10%甲醛溶液固定细胞的蛋白质时，溶解的Ni离子浓度与活细胞的溶解的Ni离子浓度相比略有降低。结果表明，种植体金属的耐腐蚀性与粘附细胞的存在密切相关。本研究对电池对金属腐蚀的影响得出以下结论。(1)金属植入物在体外的腐蚀速率，细胞粘附的存在是受控的。锚定依赖细胞（L929）促进金属离子的释放。(2)细胞的代谢活动影响金属腐蚀。失去生物活性的细胞即使粘附在金属上也不会促进金属的腐蚀。细胞粘附分子（CAM），如纤连蛋白几乎没有促进金属离子的释放。少