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Effect of the running and balance exercise on mechanoreceptor and prepheral nerve in the aged rats pads

跑步和平衡运动对老年大鼠垫机械感受器和前神经的影响

基本信息

批准号：
18500407
负责人：
KANEMURA Naohiko
金额：
$ 2.57万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500407/
关键词：
aged running exercise balance exercise mechanoreceptor 末梢神経運動

项目摘要

Neurotrophins are a family of soluble ligands that promote the survival and differentiation of the peripheral and central neurons. Recent research indicated that the treadmill exercise enhanced the expression level of neurotrophin in the spinal cord and the muscle in rats. The objective of this study was to investigate whether exercises enhance the expression level of neurotrophin in pads.Experiment 1Aged Wistar male rats, 2 years old, were randomly assigned to one of two groups: non-exercised rats and treadmill exercised rats. Animals performed quadrupedal locomotion on a treadmill until they could maintain a forward position on the belt moving at 11-13m/min Locomotor training was carried out for 4 weeks (5 days a week).In both exercised groups and non-exercised groups, NT3, NT4, TrkB, TrkC, and P75mRNA were detected in the pads.The expression TrkC and P75mRNAin the exercised group were significantly less than of non-exercised group.The expression NT3, NT4, Trk B mRNAin the exercised group were not significantly than ofnon-exercised group.Experiment 2Aged Wistar male rats (2 years old) and adult male rats (10 weeks old) were randomly assigned groups: adult with exercise, adult without exercise, aged with exercise, and aged without exercise. Animals were trained on a moving platform or 4 weeks (5 days a week).. The mRNAs for NT3, NT4, their receptors TrkB, TrkC were measured by quantitative RT-PCR. NT3 and TrkC levels of expression in aged group with exercise were more higher than that of the adult groups. The exercise increased the NT4 level TrkB levels of aged rats with excercise was high compared with adult rats with exerciseThe skin produced neurotrophins which are conveyed via retrograde axonal transport to the nerve element related neurotrophins. Athis study suggests that The attenuation of neurotrophin-Trk signaling may correlate with severity of sensory deficits in senescence.

神经营养因子是促进外周和中枢神经元存活和分化的可溶性配体家族。最近的研究表明，跑台运动增强了大鼠脊髓和肌肉中神经营养因子的表达水平。本研究的目的是探讨运动是否能增强大鼠足垫神经营养因子的表达水平。实验1将2岁的Wistar雄性大鼠随机分为两组：非运动组和跑台运动组。动物在跑步机上进行四足运动，直到它们能够在以11- 13 m/min运动的带上保持向前的位置。在运动组和非运动组中，NT 3、NT 4、TrkB、TrkC、结果运动组大鼠足垫中TrkC和P75 mRNA的表达明显低于非运动组，NT 3、P75 mRNA的表达明显低于非运动组，实验2老年Wistar雄性大鼠（2岁）和成年雄性大鼠（10周龄）随机分为成年运动组、成年非运动组、老年运动组和老年非运动组。动物在移动平台上训练4周（每周5天）。RT-PCR检测NT 3、NT 4及其受体TrkB、TrkC的mRNA水平。运动老年组NT 3和TrkC表达水平高于成年组。运动增加了老年运动大鼠的NT 4水平，TrkB水平高于成年运动大鼠，皮肤产生神经营养素，这些神经营养素通过逆行轴突运输到与神经元相关的神经营养素。本研究提示，神经营养因子-Trk信号通路的减弱可能与衰老过程中感觉功能障碍的严重程度相关。