Effects of physicul stimulation and some growth factors on peripheral nerve regeneration and muscle strophy.

物理刺激和一些生长因子对周围神经再生和肌肉萎缩的影响。

基本信息

批准号：
18500403
负责人：
MIKI Akinori
金额：
$ 2.57万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500403/
关键词：
muscle atrophy hindlimb suspension immobilization myosin heavy chain isoform electromagnetic stimulation erinheral nerve nerve regeneration nerve growth factor 末梢神経再生筋再生物理刺激褥瘡末梢血液循環

项目摘要

1) Effects of fixed muscle length during hindlimb suspension were examined. Animals were divided into 3 groups; 1: hindlimb suspension (HS), 2: hindlimb suspension with dorsal flexion (HSD), 3: hindlimb suspension with plantar flexion (HDP). In the tibias anterior, muscle atrophy occurred more severer in the shortened position than in the expanded position. While in the soleus muscle, it occurred more severer in the HS rather than HSP. These findings suggested that muscle atrophy might be depend not only on fixed muscle length but also on some other factors, such as contractile manner.2) Changes of myosin heavy chain (MHC) isoform during the hindlimb suspension were examined in the rat skeletal mus- cle. In he tibialis anterior, MHC isoforms did not change during the hindlimb suspension. In the normal soleus muscle. most of MHC was type I, but after the hindlimb suspension, rate of type I reduced and type IIx, IIa, and IIb appeared. When the electromagnetic stimulation was applied every day during the hindlimb suspension, decrease of type I and ap- pearance of type IIx, IIa and IIb was reduced. These findings suggested that in the soleus muscle, electromagnetic sti- mulation might lighten not only muscle strophy but also immaturelization of myosin heavy chain isoformes.3) Expression of nerve growth factor (NGF) in the peripheral nerve during regeneration was immunohistochemically examined. In the normal nerve, NGF was not noted, but in the crushed nerve. NGF immunoreaction became to be found.When the electromagnetic stimulation was applied to the regenerating nerve, the immunoreaction became stronger. It was said that, the electromagnetic stimulation accelerate the nerve regeneration. One of the reasons for the effect of the stimulation might be the enhancement of the expression of NGF through electromagnetic stimulation.

1）检查了后肢悬浮液期间固定肌肉长度的影响。动物分为3组； 1：后肢悬架（HS），2：具有背屈曲（HSD）的后肢悬架，3：带有足底屈曲（HDP）的后肢悬架。在胫骨前，肌肉萎缩在缩短的位置比在扩展的位置更为严重。在比目鱼肌肉中，它在HS而不是HSP中更为严重。这些发现表明，肌肉萎缩可能不仅取决于固定的肌肉长度，还取决于其他因素，例如收缩的方式。2）在大鼠骨骼肌中检查了后肢悬浮液期间肌球蛋白重链（MHC）同工型的变化。在胫骨前部，MHC同工型在后肢悬架期间没有改变。在正常的比目鱼肌肉中。 MHC的大部分是I型，但是在后肢悬架之后，I型降低和类型IIX，IIA和IIB出现。当在后肢悬浮液中每天应用电磁刺激时，IIA和IIB型I型和IIA型的IIA和IIB的表现减少。这些发现表明，在比目鱼肌中，电磁态不仅可以减轻肌肉茎，而且还会减轻肌球蛋白重链同工型的不成熟。3）在再生过程中周围神经中神经生长因子（NGF）的表达。在正常神经中，没有注意到NGF，而是在碎神经中。 NGF免疫反应已被发现。当将电磁刺激应用于再生神经时，免疫反应变得更强。据说，电磁刺激加速了神经再生。刺激作用的原因之一可能是通过电磁刺激增强NGF的表达。