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Effects of physicul stimulation and some growth factors on peripheral nerve regeneration and muscle strophy.

物理刺激和一些生长因子对周围神经再生和肌肉萎缩的影响。

基本信息

批准号：
18500403
负责人：
MIKI Akinori
金额：
$ 2.57万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500403/
关键词：
muscle atrophy hindlimb suspension immobilization myosin heavy chain isoform electromagnetic stimulation erinheral nerve nerve regeneration nerve growth factor 末梢神経再生筋再生物理刺激褥瘡末梢血液循環

项目摘要

1) Effects of fixed muscle length during hindlimb suspension were examined. Animals were divided into 3 groups; 1: hindlimb suspension (HS), 2: hindlimb suspension with dorsal flexion (HSD), 3: hindlimb suspension with plantar flexion (HDP). In the tibias anterior, muscle atrophy occurred more severer in the shortened position than in the expanded position. While in the soleus muscle, it occurred more severer in the HS rather than HSP. These findings suggested that muscle atrophy might be depend not only on fixed muscle length but also on some other factors, such as contractile manner.2) Changes of myosin heavy chain (MHC) isoform during the hindlimb suspension were examined in the rat skeletal mus- cle. In he tibialis anterior, MHC isoforms did not change during the hindlimb suspension. In the normal soleus muscle. most of MHC was type I, but after the hindlimb suspension, rate of type I reduced and type IIx, IIa, and IIb appeared. When the electromagnetic stimulation was applied every day during the hindlimb suspension, decrease of type I and ap- pearance of type IIx, IIa and IIb was reduced. These findings suggested that in the soleus muscle, electromagnetic sti- mulation might lighten not only muscle strophy but also immaturelization of myosin heavy chain isoformes.3) Expression of nerve growth factor (NGF) in the peripheral nerve during regeneration was immunohistochemically examined. In the normal nerve, NGF was not noted, but in the crushed nerve. NGF immunoreaction became to be found.When the electromagnetic stimulation was applied to the regenerating nerve, the immunoreaction became stronger. It was said that, the electromagnetic stimulation accelerate the nerve regeneration. One of the reasons for the effect of the stimulation might be the enhancement of the expression of NGF through electromagnetic stimulation.

1)考察固定肌肉长度对后肢悬吊的影响。动物分为3组；1：后肢悬吊（HS）， 2：后肢悬吊伴背屈（HSD）， 3：后肢悬吊伴足底屈（HDP）。在胫骨前侧，肌萎缩在短位时比在扩张位时更为严重。而在比目鱼肌中，HS比HSP更严重。这些结果表明，肌肉萎缩可能不仅与固定肌肉长度有关，还与收缩方式等其他因素有关。2)观察大鼠后肢悬吊过程中肌球蛋白重链（MHC）异构体的变化。在胫骨前肌，MHC亚型在后肢悬吊期间没有改变。在正常的比目鱼肌中。MHC以I型居多，后肢悬吊后I型比例减少，出现IIx、IIa、IIb型。后肢悬吊期间每天给予电磁刺激，ⅰ型减少，IIx、IIa、IIb型出现减少。这些结果表明，在比目鱼肌中，电磁刺激不仅可以减轻肌肉萎缩，还可以减轻肌球蛋白重链异构体的不成熟。3)采用免疫组织化学方法检测再生过程中周围神经中神经生长因子（NGF）的表达。正常神经中未见NGF，受压神经中可见。开始发现NGF免疫反应。当电磁刺激作用于再生神经时，免疫反应增强。据说，电磁刺激能加速神经的再生。产生这种效果的原因之一可能是通过电磁刺激增强了NGF的表达。