Influence of electrical and electromagnetic stimulation on peripheral nerve regeneration

电和电磁刺激对周围神经再生的影响

• 批准号: 12832025
• 负责人: MIKI Akinori
• 金额: $ 2.43万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12832025/
• 关键词: Nerve regeneratiion; Electrical stimulation; Electromagnetic stimulation; Peripheral nerve; Morphology; 形態的観察; マウス坐骨神経; 電子顕微鏡

Influence of the electrical and electromagnetic stimulation on nerve regeneration was morphologically examined in the injured mouse sciatic nerve. Two days after the transection, the electrical and electromagnetic stimulation was applied to the injured sciatic nerve. In the regenerating nerve fibers, only the growth cones were selectively damaged, but the axonal shaft of the regenerating fibers appeared to be normal. To examine the influence of the stimulation on maturation of regenerating nerve fibers, electrical and electromagnetic stimulation was applied every day to the regenerating sciatic nerves, and diameter and the number of the remyelinated regenerating nerve fibers were counted at 5mm distal to the crush end of the sciatic nerve on 7th , 14th , 21th and 28th days after the begining of the stimulation. On the 7th and 14th days, the number and diameter of the remyelinated regenerating fibers in the stimulated groups were more than in the control group (without stimulation). It is suggested that the rate of extension and maturation of the regenerating nerve fibers are promoted by the electrical and electromagnetic stomulation. But, on 28th day, the number of the regenerating nerve fibers in the stimulated groups was much less than that in the control group.

采用小鼠坐骨神经损伤模型，观察电刺激和电磁刺激对神经再生的影响。术后2天对损伤的坐骨神经进行电刺激和电磁刺激。在再生神经纤维中，只有生长锥体被选择性损伤，但再生纤维的轴突似乎正常。为观察刺激对再生神经纤维成熟的影响，每天对再生坐骨神经进行电刺激和电磁刺激，分别于刺激开始后第7、14、21、28天在坐骨神经损伤端远端5 mm处计数有髓再生神经纤维直径和数目。在第7、14天，刺激组有髓再生纤维的数量和直径均多于对照组(无刺激)。提示电造口术和电磁造口术可促进再生神经纤维的伸展和成熟。但在第28天，刺激组再生神经纤维的数量明显少于对照组。

项目成果

Akiko OGATA: "Influence of electrical and electromagnetic stimulation on a nerve regeneration in the transected mouse sciatic nerve"Bull.Health Sci.Kobe. 17. 13-22 (2001)

Akiko OGATA：“电和电磁刺激对横切小鼠坐骨神经神经再生的影响”Bull.Health Sci.Kobe。

Akiko OGATA et al: "Influence of electrical and electromagnetic stimulation on nerve regeneration in the transected mouse sciatic nerve"Bull. Health Sci. Kobe. 17. 13-22 (2001)

Akiko OGATA 等人：“电和电磁刺激对横切小鼠坐骨神经神经再生的影响”Bull。

Akiko OGATA: "Influence of electrical and electromagnetic stimulation on nerve regeneration in the transected mouse sciatic nerve"Bull. Heatlh Sci Kobe. 17. 13-22 (2001)

Akiko OGATA：“电和电磁刺激对横切小鼠坐骨神经神经再生的影响”Bull。

田口徹: "下り坂走行による運動負荷後にみられるマウス下腿三筋における血行動態の経時的変化"理学療法の医学的基礎研究会雑誌. 5(印刷中). (2001)

Toru Taguchi：“下坡跑步造成的运动压力后小鼠小腿三块肌肉血流动力学的时间变化”《物理治疗研究组医学基础》杂志 5（出版中）。