MORPHOLOGICAL AND PHYSIOLOGICAL STUDY ON EFFECTS OF ELECTRIC STIMULATION ON PERIPHERAL NEURO-MUSCULAR PARALYSIS.

电镜观察了电刺激对小鼠坐骨神经近端残端修复过程和神经再生的影响。细胞器的积累（终末积累）和分界膜的形成在近端残端横断后不久开始。但囊泡积聚部分不能在髓鞘中长期保存。可见，当轴突残端被髓鞘包围时，囊泡积聚、分界膜的形成和囊泡积聚部分的退化可能会重复发生。这种修复过程对于尽量减少外源因素引起的破坏性变化可能是非常重要的，通过这种措施，活的轴突部分总是与退化的轴突部分分开，最终被丢弃。横断后5-6小时，位于切端近端的朗维耶结开始形成轴突芽。在雪旺细胞髓鞘和基底层之间，有更多的轴突向远端延伸。横断后不久进行电刺激。横断后6 h， Ranvier结处出现轴突萌发。实验组与对照组的神经再生早期过程无显著差异。横断后6小时或1天。横断后2天进行电刺激。在控制中。许多再生轴突向远端延伸，超出切割端。几乎所有再生的轴突在形态上都是正常的。电刺激3 h后，仅再生轴突的部分生长锥出现囊泡和线粒体破裂、多泡体和溶酶体形成等破坏性变化。刺激后1天，实验组与对照组的神经再生无显著差异。这些发现表明，电刺激可能只是短暂的影响，主要是对新生的生长锥。少

Repairing process and effects of electric stimulation on nerve regeneration were electron microscopically studied at the proximal stump of the transected mouse sciatic nerve. Accumulation of cell organelles (terminal accumulation) and formation of demarcation membranes began soon after transection at the proximal stump. But the vesicle- accumulated portions could not survive for long in the myelin sheath. It appears that vesicle accumulation, formation of the demarcation membranes and degeneration of the vesicle-accumulated portions might be repeated as far as the axon stumps are enclosed by myelin sheath. This repairing process might be very important to minimize the destructive changes which night be caused by exogenic factors, and with this measure, the living axonal portions are always separated from the degenerating portions which are eventually cast off.Axonal sprout formation began 5-6 hours after transection at the node of Ranvier locating just proximal to the cut end. Regenera … More ting axons extensed distally between the myellin sheath and basal lamina of Schwann cells. Electric stimulation was performed soon after the transection. But the axonal sprouting occurred at the node of Ranvier 6 hours after the transection. There were no significant differences in the early process of nerve regeneration between experimental and control groups. both 6 hours or one day after transection. The electric stimulation was performed two days after transection. In the controls. many regenerating axons extended distally beyond , the cut end. Almost all regenerating axons appeared to be morphologically normal. Three hours after the electric stimulation, destructive changes such as rupure of vesicles and mitochondria, formation of multivesicular bodies and lysosomes were observed only in some growth cones of the regenerating axons. One day after the stimulation, there were no significant differences in nerve regeneration between the experimental and control groups. These findings suggest that the electric stimulation might affect only transiently and mainly on nascent growth cones. Less