MORPHOLOGICAL AND PHYSIOLOGICAL STUDY ON EFFECTS OF ELECTRIC STIMULATION ON PERIPHERAL NEURO-MUSCULAR PARALYSIS.

电刺激对周围神经肌肉麻痹影响的形态学和生理学研究。

• 批准号: 09671494
• 负责人: MIKI Akinori
• 金额: $ 2.3万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671494/
• 关键词: REPAIRING PROCESS; NERVE REGENERATION; MYELINATED AXONS; MOUSE SCIATIC NERVE; ELECTRIC STIMULATION; ELECTRON MICROSCOPY; Mouse sciatic nerue; Electric stimulation; 神経再生; 筋麻痺; 電気刺激療法; 末梢神経; 骨格筋

Repairing process and effects of electric stimulation on nerve regeneration were electron microscopically studied at the proximal stump of the transected mouse sciatic nerve. Accumulation of cell organelles (terminal accumulation) and formation of demarcation membranes began soon after transection at the proximal stump. But the vesicle- accumulated portions could not survive for long in the myelin sheath. It appears that vesicle accumulation, formation of the demarcation membranes and degeneration of the vesicle-accumulated portions might be repeated as far as the axon stumps are enclosed by myelin sheath. This repairing process might be very important to minimize the destructive changes which night be caused by exogenic factors, and with this measure, the living axonal portions are always separated from the degenerating portions which are eventually cast off.Axonal sprout formation began 5-6 hours after transection at the node of Ranvier locating just proximal to the cut end. Regenera … More ting axons extensed distally between the myellin sheath and basal lamina of Schwann cells. Electric stimulation was performed soon after the transection. But the axonal sprouting occurred at the node of Ranvier 6 hours after the transection. There were no significant differences in the early process of nerve regeneration between experimental and control groups. both 6 hours or one day after transection. The electric stimulation was performed two days after transection. In the controls. many regenerating axons extended distally beyond , the cut end. Almost all regenerating axons appeared to be morphologically normal. Three hours after the electric stimulation, destructive changes such as rupure of vesicles and mitochondria, formation of multivesicular bodies and lysosomes were observed only in some growth cones of the regenerating axons. One day after the stimulation, there were no significant differences in nerve regeneration between the experimental and control groups. These findings suggest that the electric stimulation might affect only transiently and mainly on nascent growth cones. Less

通过电子显微镜研究了横断小鼠坐骨神经近端残端的修复过程和电刺激对神经再生的影响。在近端残端横断后不久，细胞器的积累（末端积累）和分界膜的形成就开始了。但囊泡积累的部分不能在髓鞘中长期存活。看来，只要轴突残端被髓鞘包围，囊泡积累、分界膜的形成和囊泡积累部分的变性可能会重复。这种修复过程对于最大限度地减少外源因素造成的破坏性变化可能非常重要，通过这种措施，活的轴突部分总是与最终脱落的退化部分分离。轴突芽形成在横断后 5-6 小时开始，位于紧邻切割端的 Ranvier 节点。再生轴突在雪旺细胞的髓鞘和基底层之间向远端延伸。横断后不久进行电刺激。但横断后6小时，Ranvier结处出现轴突出芽。实验组和对照组神经再生的早期过程没有显着差异。横切后 6 小时或一天。横断后两天进行电刺激。在控件中。许多再生轴突向远端延伸超出切割端。几乎所有再生轴突的形态均正常。电刺激三小时后，仅在再生轴突的一些生长锥中观察到破坏性变化，例如囊泡和线粒体的破裂、多囊泡体和溶酶体的形成。刺激一天后，实验组和对照组之间的神经再生没有显着差异。这些发现表明，电刺激可能只会短暂地影响，并且主要影响新生的生长锥。较少的

项目成果

A.Miki,T.Matsubara,and Y.Kohbu: "Repairing process at the proximal stump of myelinated axons, with special reference to fomation of the demarcaion memboanes." Bull.Health Sci. Kobe. 14. 11-23 (1998)

A.Miki、T.Matsubara 和 Y.Kohbu：“有髓轴突近端残端的修复过程，特别是分界膜的形成。”

A.Miki, T.Matsubara, and Y.Khobu: "Repaining process at the proximal stuanp of myelinated arons, with special reference to formation of the demar cation membranes." Ball. Health Sci, Kobe. 14. 11-23 (1998)

A.Miki、T.Matsubara 和 Y.Khobu：“有髓鞘近端的修复过程，特别是分界膜的形成。”

AKINORI MIKI,TAKAKO MATSUBARA,YOSHIHIDE KOHBU: "REPAIRING PROCESS AT THE PROXIMAL STUMP OF TRANSECTED MYELINATED AXONS,WITH SPECIAL REFERENCE TO FORMATION OF THE DEMARCATION MEMBRANES" BULL.HEALTH SCI.KOBE. 14. 11-23 (1998)

AKINORI MIKI、TAKAKO MATSUBARA、YOSHIHIDE KOHBU：“横断髓鞘轴突近端残端的修复过程，特别参考分界膜的形成”BULL.HEALTH SCI.KOBE。