Sensitive assay for disturbance of brain function by environmental pollutants at low concentrations

低浓度环境污染物对脑功能干扰的灵敏测定

• 批准号: 18510058
• 负责人: YAMAZAKI Takeshi
• 金额: $ 2.68万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18510058/
• 关键词: environmental pollution; tributyltin; working memory; estradiol; neurosteroid; オペラント条件付け; 環境汚染物質; メチル水銀

Tributyltin induced increase in cytochromes P45017α and P450arom in cultures rat hippocampal slices. This treatment also induced increase in biosynthesis of testosterone and estradiol 17β. Since tributyltin can activate RXR-mediating cellular response, we determined action of RXR on neuronsteroidogenesis in the hippocampus. 9-cis Retinoic acid, an agonist for RXR, stimulated estradiol synthesis and mRNA contents for P450 17α in cultured rat hippocampus. These results indicate that the action of tributyltin on hippocampal steroidogenesis was mediated by RXR.Direct injection of tributyltin in adult rat hippocampus induced increase in mRNA for P450 17α. Estradiol content of the hippocampus, however, was not affected, although the treatment with tributyltin may stimulate testosterone biosynthesis in the hippocampus. Castration of the rat induced significant decrease in testosterone contents in brain and serum. Estradiol content in the castrated rat hippocampus was the same as that in the non-treated rat. These data indicate that the estradiol content in the hippocampus was independent from testosterone content and synthesis in the hippocampus.

三丁基锡诱导培养的大鼠海马片细胞色素P45017α和P450arom增加。该处理还诱导睾酮和雌二醇17β的生物合成增加。由于三丁基锡可以激活RXR介导的细胞反应，我们确定了RXR在海马神经元类固醇生成中的作用。RXR激动剂9-顺式维甲酸可促进培养的大鼠海马神经元雌激素合成和P45017α的表达。这些结果表明，三丁基锡对大鼠海马类固醇合成的作用是通过RXR介导的。成年大鼠海马区直接注射三丁基锡可诱导P45017α基因表达增加。然而，尽管三丁基锡治疗可能会刺激海马体中睾酮的生物合成，但海马体中雌二醇的含量并未受到影响。去势后大鼠脑和血清中睾酮含量显著降低。去势大鼠海马区雌二醇含量与未治疗组相同。这些数据表明，海马区的雌二醇含量与海马区的睾酮含量和合成无关。

项目成果

Dephosphorylation of TORC initiates expression of the StAR gene

• DOI: 10.1016/j.mce.2006.12.020
• 发表时间: 2007-02
• 期刊: Molecular and Cellular Endocrinology
• 影响因子: 4.1
• 作者: H. Takemori;Mariko Kanematsu;Junko Kajimura;O. Hatano;Y. Katoh;Xing-zi Lin;L. Min;T. Yamazaki;J. Doi;M. Okamoto

環境化学物質暴露によるラット新生仔脳および生殖器官におけるエストロゲンレセプターの発現への影響

环境化学物质暴露对新生大鼠脑及生殖器官雌激素受体表达的影响

• 发表时间: 2006
• 作者: 柳田 真希

Effect of methyl mercury on mRNA contents of estradiol synthetic enzymes in rat hippocampus

甲基汞对大鼠海马雌二醇合成酶mRNA含量的影响

• 发表时间: 2006
• 作者: Syota;Komatsu
• 通讯作者: Komatsu

MALDI-TOF Mass Spectrometric Investigation on the Interaction of Cytochrome P450 17α with NADPH-Cytochrome P450 Reductase using Chemical Modification

使用化学修饰的 MALDI-TOF 质谱研究细胞色素 P450 17α 与 NADPH-细胞色素 P450 还原酶的相互作用

• 发表时间: 2006
• 作者: Takeshi;Yamazaki;Laleh Nikfarjam,
• 通讯作者: Laleh Nikfarjam,

Rapid modulation of long-term depression and spinogenesis via synaptic estrogen receptors in hippocampal principal neurons

• DOI: 10.1111/j.1471-4159.2006.04264.x
• 发表时间: 2007-02-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Mukai, Hideo;Tsurugizawa, Tomokazu;Kawato, Suguru
• 通讯作者: Kawato, Suguru