The network of regulatory interactions of MADS-box proteins during floral organ development in Arabidopsis thaliana

在拟南芥中，MADS盒转录因子家族包括100多个成员。最有名的和最深入的研究是花同源异型MADS盒基因，控制发展和建立两个相邻的花轮的器官身份。这些MADS蛋白对花器官发生的重要性表现在器官的显著表型改变中，当相应的基因被突变失活时。MADS蛋白的基本结构除了与DNA结合外，还允许形成多种蛋白质-蛋白质相互作用，这些相互作用似乎是复杂调控网络的基础。到目前为止，关于拟南芥花生殖发育过程中MADS盒基因在器官识别基因下游的作用还知之甚少。我们希望确定和分析MADS盒基因调控生殖过程中的拟南芥。完全测序的基因组允许快速在计算机上分离新的MADS盒基因。通过表达模式的研究，MADS盒基因可以选择在雄蕊和心皮发育过程中主要表达。为了研究它们在控制生殖生长的调控网络中的功能，我们希望应用基于酵母的技术来分离它们的相互作用伙伴并分离它们的下游靶基因。Frau Dr. Sabine Zachgo ist Co-Principal des Projekts电话：0221 5062190 E-Mail：szachgo@mpiz-koeln.mpg.de

In Arabidopsis, the MADS-box family of transcription factors comprises more than 100 members. The best known and most intensively studied are the floral homeotic MADS-box genes which control development and the establishment of organ identity in two neighboring floral whorls. The importance of these MADS proteins for floral organogeneses is manifested in the dramatic phenotypic alterations of organs, when the respective genes are inactivated by mutations. The basic structure of the MADS proteins allows, besides their binding to DNA, to form multiple protein-protein interactions that seem to be the basis of complex regulatory networks. So far, only little is known about MADS-box genes acting downstream of the organ identity genes during floral reproductive development in Arabidopsis. We want to identify and analyze MADS-box genes that regulate reproductive processes in Arabidopsis. The fully sequenced genome allows quick in silico isolation of new MADS-box genes. By expression pattern studies MADS-box genes can be selected that are predominantly expressed during stamen and carpel development. In order to investigate their function within the regulatory network controlling reproductive growth, we want to apply yeast-based techniques to isolate their interaction partners and isolate their downstream target genes.Frau Dr. Sabine Zachgo ist Co-Principal des Projekts Tel.: 0221 5062190E-Mail: szachgo@mpiz-koeln.mpg.de