Development of Hg(II)-trapping technology based on the physicochemical properties of the T-Hg(II)-T base-pair

最近，粮农组织和世卫组织警告说，大型鱼类体内累积的汞含量(特别是在鱼体中)已达到对胚胎有害的水平。为了减少汞在鱼类中的积累，降低环境汞的浓度(总量)是很重要的。为此，已经提出了相当多的从环境中捕获汞(II)的技术，例如用碘(R)沉淀，用EDTA相关化合物或半胱氨酸修饰的树脂对汞(II)进行螯合，以及细菌捕获汞(生物修复)。然而，这些技术并不是专门针对汞(II)离子的。在这个项目中，我们提出了一种新的汞(II)捕集技术，这种技术对汞(II)具有高度的专一性。这项技术依赖于我们小组的一项知识，即汞(II)被DNA双链中T-T错配中的胸腺嘧啶(T)残基捕获(J.Am Chem Soc.，2006,128,2172-2173)。因此，这笔赠款的获得者(田中)推测，通过使用用寡聚-T改性的树脂将…更多的工作是作为一种汞(II)捕捉器。然后，他合成了寡聚T改性的聚苯乙烯树脂，并用电感耦合等离子体光谱检测了其对汞(II)的捕集效率。结果表明，在其他金属配料存在或不存在的情况下，该寡核苷酸修饰的聚苯乙烯树脂(Hg(II))捕集装置能有效地捕集溶液中的Hg(II)。更有趣的是，这种汞(II)捕捉器专门捕获汞(II)，而不显著捕获任何其他金属阳离子。此外，获奖者还在外部进行了T-Hg(II)T碱基对的结构测定和物理化学表征，以促进开发更有效的汞(II)捕获装置，并澄清它们的适当序列。特别是用~(15)N核磁共振测定T-Hg(II)T碱基对的化学结构(J.Am Chem Soc.，2007,129,244-245.)在世界范围内对核酸-金属相互作用领域产生了影响，获奖者被邀请为。书中章节的作者(N.Hadjiliadis和E.Sletten编辑，《金属络合物-DNA相互作用》，布莱克韦尔出版社，英国牛津。)和一个个人账户(道尔顿交易)。最后，获奖者揭示了T-Hg(II)T碱基盘的基本性质，并基于T-Hg(II)T碱基对的基础科学创造了一种汞(II)捕集装置。较少

Recently, FAO and WHO warned that the amount of mercury accumulated in large fishes (especially in tune) became a toxic level against embryos. In order to reduce mercury accumulated in fishes, it is important to reduce the concentration (total amount) of environmental mercury. For this purpose, considerable numbers of techniques to capture Hg(II) from environment have been proposed, such as precipitation with iodide (r), chelation of Hg(II) by resins modified with EDTA-related compounds or cysteine, and bacterial trapping of mercury (bio-remediation). However, these techniques are not specific for Hg(II) ions. In this project, we propose a new technology for a Hg(II)trapping which is highly specific for Hg(II). This technique rely on a knowledge in our group that Hg(II) is captured by thymine (T) residues in T -T mismatches in DNA duplexes (J. Am Chem Soc., 2006, 128, 2172-2173.). Therefore, the awardee of this grant (Tanaka) postulated that by using resins modified with oligo-T would … More work as a Hg(II)trapping device. He then synthesized the oligo-T-modified poly-stylene resin, and examine its Hg(II)-trapping efficiency with ICP spectroscopy. It was found that this oligo-T modified poly-stylene resin (Hg(II) trapping device) efficiently trapped Hg(II) from solution in the presence or absence of other metal rations. More interestingly, this Hg(II)trapping device specifically captured Hg(II) without trapping any other metal cations significantly. In addition, the awardee performed structure determination and physicochemical characterizations of T-Hg(II) T base-pairs, in outer to facilitate developments of more efficient Hg(II) trapping devices and to clarify appropriate sequences for them. Especially, the determination of the chemical structure of T-Hg(II) T base-pairs with ^15N NMR (J. Am Chem Soc., 2007, 129, 244-245.) gave impact to the field of nucleic acids-metal interactions in world-wide manner, and the awardee was invited as. A writer for a book chapter (N. Hadjiliadis and E. Sletten Eds., "Metal Complexes - DNA Interactions", Blackwell Publishing, Oxford, UK.) and a personal account (Dalton Transactions). In conclusion, the awardee revealed the fundamental properties of the T-Hg(II) T base-pan and created a Hg(II)-trapping device based on basic sciences of the T-Hg(II) T base-pairs. Less