Search on the genome scale of mRNAs that are translated under the abiotic stresses

在高温、高盐、干旱和缺氧等多种非生物胁迫条件下，植物的翻译结构发生了剧烈的变化。在翻译水平上对上述应激反应的共同特征是翻译的全局抑制和部分差异调节mrna的存在，这些mrna对这种抑制是不耐受的。然而，与转录水平相比，我们对翻译水平的调控或调控网络知之甚少。在这项研究中，我们利用DNA芯片对拟南芥（Arabidopsis thaliana）悬浮细胞培养在高温（热胁迫）和高盐（盐胁迫）条件下的翻译调控进行了全基因组分析，比较了刺激前后单个mrna的翻译状态。在此基础上，比较了两种应力条件下的平移规律。与以往只关注单一非生物胁迫的报道相比，这一比较分析有望更好地理解植物对非生物胁迫反应的翻译控制机制、调控网络或生物学意义。结果表明，高温和高盐度均可引起细胞对大多数mRNA的翻译抑制，而一些mRNA对翻译抑制不耐受或敏感。值得注意的是，我们发现在植物对两种胁迫的反应中，单个mRNA物种在翻译水平上的行为是相似的。

Drastic translational changes have been observed in plants under various abiotic stress conditions such as elevated temperature, high salinity, drought, and O2 deprivation. Common features of responses at the translational level to above stresses are global repression of translation and the existence of a fraction of differentially regulated mRNAs that are refractory to the repression. Less is known, however, about the regulation or regulatory networks at the translational levels compared to the transcriptional levels.In this study, we carried out genome-wide analysis of translational regulation in Arabidopsis (Arabidopsis thaliana) suspension cell culture subjected to elevated temperature (heat stress) and high salinity (salt stress) conditions using DNA microarray by comparing the translation state of individual mRNAs before and after the application of a stimulus. On that basis, translational regulations under the both stress conditions were compared. It was expected that this comparative analysis would allow gaining a better understanding of the mechanism, the regulatory network, or biological significance of translational control in plants response to abiotic stress than previous reports focused on a single abiotic stress. Our results showed that both elevated temperature and high salinity elicit the cells to translation repression of most mRNA species, while some were defied to or sensitive to the repression. Notably, we found that the behavior of individual mRNA species at the translational levels were similar between in plants response to both stresses.