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Search on the genome scale of mRNAs that are translated under the abiotic stresses

在非生物胁迫下翻译的 mRNA 的基因组规模搜索

基本信息

批准号：
18580338
负责人：
KATO Ko
金额：
$ 2.53万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580338/
关键词：
Translational regulation Abiotic stress Arabidopsis thaliana DNA microarray Polvsome profile

项目摘要

Drastic translational changes have been observed in plants under various abiotic stress conditions such as elevated temperature, high salinity, drought, and O2 deprivation. Common features of responses at the translational level to above stresses are global repression of translation and the existence of a fraction of differentially regulated mRNAs that are refractory to the repression. Less is known, however, about the regulation or regulatory networks at the translational levels compared to the transcriptional levels.In this study, we carried out genome-wide analysis of translational regulation in Arabidopsis (Arabidopsis thaliana) suspension cell culture subjected to elevated temperature (heat stress) and high salinity (salt stress) conditions using DNA microarray by comparing the translation state of individual mRNAs before and after the application of a stimulus. On that basis, translational regulations under the both stress conditions were compared. It was expected that this comparative analysis would allow gaining a better understanding of the mechanism, the regulatory network, or biological significance of translational control in plants response to abiotic stress than previous reports focused on a single abiotic stress. Our results showed that both elevated temperature and high salinity elicit the cells to translation repression of most mRNA species, while some were defied to or sensitive to the repression. Notably, we found that the behavior of individual mRNA species at the translational levels were similar between in plants response to both stresses.

在高温、高盐、干旱、缺氧等非生物胁迫条件下，植物的翻译水平发生了急剧的变化。在翻译水平上对上述应激的反应的共同特征是翻译的整体抑制和存在一部分对抑制不敏感的差异调节的mRNA。然而，与转录水平相比，对翻译水平的调控或调控网络知之甚少。我们对拟南芥中的翻译调控进行了全基因组分析，（拟南芥）悬浮细胞培养物经受高温（热胁迫）和高盐度通过比较施加刺激之前和之后的单个mRNA的翻译状态，使用DNA微阵列在盐胁迫（salt stress）条件下测定mRNA的翻译水平。在此基础上，比较了两种胁迫条件下的翻译调控。与以往报道的单一非生物胁迫相比，本文的比较分析将有助于更好地理解植物对非生物胁迫响应的翻译调控机制、调控网络或生物学意义。结果表明，高温和高盐胁迫均能引起大多数mRNA的翻译抑制，但也有一些mRNA对这种抑制不敏感或不敏感。值得注意的是，我们发现，在翻译水平上的单个mRNA种类的行为是相似的，在植物对这两种胁迫的反应之间。