Expression of LXR and regulation of glucose and fat metabolism in human cancers

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In the present research, we examined expression of LXR α in human cancer cell lines and in some resected human cancers.1. Expression of LXR α in human cancer cell linesImmunohistochemical examination revealed expression of LXR α in five cell lines among eight gastric cancer cell lines.Pancereatic cancer cell lines (n=4), one melanoma cell line and one myeloma cell line lacked LXR α LBD expression.2. Expression of LXR a in human cancersOne case in lung carcinoma (n=55), two cases in seminomas/dysgerminomas (n=42)and two cases in gastric carcinomas (n=30) showed positivity for LXR α.3. Expression of LXR amRNAThree gastric cancer cell lines examined (KATOM, MKN28 and MKN45)expressed LXR α mRNA. Accordingly. mRNA for RXRα/β,PEAR α, SREBP1c/la were detected in MKN28 cells. This implies that at least in MKN28, pathway from LXR α -RXR-SREBP1c to PPAR α has been established.4. Inhibition of LXR α expression by iRNA in MKN28 caused no morphological change and growth rate of the cells.5. Preliminarily, antagonist of LXR α (T0901317)induced growth retardation of MKN28 cells. In conclusion, expression of LXR α in human cancers is rare event but overexpressed LXR a might be a target of cancer therapy.