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Expression of LXR and regulation of glucose and fat metabolism in human cancers

人类癌症中 LXR 的表达及葡萄糖和脂肪代谢的调节

基本信息

批准号：
18590337
负责人：
SUZUKI Toshimitsu
金额：
$ 2.52万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590337/
关键词：
LXRα human cancer cell lines LXR α m RNA immunohistochemistry RXR SREBP-1c iRNA LXRα aeonist SREBP-1 脂肪酸合成酵素グルコーストランスポーター

项目摘要

In the present research, we examined expression of LXR α in human cancer cell lines and in some resected human cancers.1. Expression of LXR α in human cancer cell linesImmunohistochemical examination revealed expression of LXR α in five cell lines among eight gastric cancer cell lines.Pancereatic cancer cell lines (n=4), one melanoma cell line and one myeloma cell line lacked LXR α LBD expression.2. Expression of LXR a in human cancersOne case in lung carcinoma (n=55), two cases in seminomas/dysgerminomas (n=42)and two cases in gastric carcinomas (n=30) showed positivity for LXR α.3. Expression of LXR amRNAThree gastric cancer cell lines examined (KATOM, MKN28 and MKN45)expressed LXR α mRNA. Accordingly. mRNA for RXRα/β,PEAR α, SREBP1c/la were detected in MKN28 cells. This implies that at least in MKN28, pathway from LXR α -RXR-SREBP1c to PPAR α has been established.4. Inhibition of LXR α expression by iRNA in MKN28 caused no morphological change and growth rate of the cells.5. Preliminarily, antagonist of LXR α (T0901317)induced growth retardation of MKN28 cells. In conclusion, expression of LXR α in human cancers is rare event but overexpressed LXR a might be a target of cancer therapy.

在本研究中，我们检测了LXR α在人类癌细胞系和一些切除的人类癌症中的表达. LXR α在人胃癌细胞系中的表达免疫组化结果显示，8种胃癌细胞系中有5种细胞系表达LXR α，其中4种胰腺癌细胞系、1种黑色素瘤细胞系和1种骨髓瘤细胞系均不表达LXR α LBD. LXR α在肺癌（n=55）、恶性淋巴瘤/无性细胞瘤（n=42）和胃癌（n=30）中的表达分别为1例、2例。LXR α mRNA的表达检测的三种胃癌细胞系（KATOM、MKN 28和MKN 45）均表达LXR α mRNA。相应地在MKN 28细胞中检测到RXRα/β、PEAR α、SREBP 1c/1a的mRNA。这意味着至少在MKN 28中，已经建立了从LXR α -RXR-SREBP 1c到PPAR α的通路. iRNA抑制MKN 28细胞中LXR α的表达后，细胞形态和生长速度均无明显变化. LXR α拮抗剂（T0901317）可诱导MKN 28细胞生长迟缓。总之，LXR α在人类肿瘤中的表达是罕见的，但过表达LXR α可能是肿瘤治疗的靶点。