Elucidation of molecular structure of lipid peroxides as oxidative suns markers and and application of those to legal medicine

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1. Identification of molecular structure of hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH)Hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH), epoxyhydroxy-PC, oxo-PC, trihydroxy-PC were identified in human plasma by LC/ESI-MS and LC/ESI-MS-MS. Hydroperoxy lyso-phosphatidylcholine (18:2-OOH) and lyso- phosphatidylcholine oxide (oxo-18:2) were also identified. 18:2, 20:4, 16:0, 18:1, and 18:0 were identified as molecular species of lyso-phosphatidylcholine.2. Oxidative stress identified by oxisterols as a marker(1) Alcoholic disorder in rat small intestine and anti-oxidative effect of daizeinAcute ethanol feeding induced accumulation of cholesterol hydroper-oxide and oxysterols in rat small intestine, suggesting that ethanol loading increased oxidative stress. Loading of Daisein in advance canceled such effects.(2) Fasting stress in ratOxidative stress due to fasting was stronger in the first day after fasting than in the second day in rat.(3) Oxidative stresss due to ischemic-reperfusion in turniquet-releaseThe enhanced oxidative stresss due to ischemic-reperfusion in turniquet-release mice were observed not only in the gastrocnemius muscles but also in the kidneys and liver.(4) Liver disorder by D-galactosamine and the effect of anti-oxidantD-galactosamine loading induced accumulation of cholesterol hydroperoxide and oxysterols in rat liver, brain and gastrocnemius muscles, suggesting that oxidative stress by D-galactosamine depend on organs or tissues.