Elucidation of molecular structure of lipid peroxides as oxidative suns markers and and application of those to legal medicine

阐明作为氧化太阳标记的脂质过氧化物的分子结构及其在法律医学中的应用

• 批准号: 18590633
• 负责人: UENO Yasuhiro
• 金额: $ 2.44万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590633/
• 关键词: lipid peroxidation; hydroperoxy phos hatidvlcholine; alcohol; fasting; oxidative stress; oxysterol; anti-oxidant; turniouet-release

1. Identification of molecular structure of hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH)Hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH), epoxyhydroxy-PC, oxo-PC, trihydroxy-PC were identified in human plasma by LC/ESI-MS and LC/ESI-MS-MS. Hydroperoxy lyso-phosphatidylcholine (18:2-OOH) and lyso- phosphatidylcholine oxide (oxo-18:2) were also identified. 18:2, 20:4, 16:0, 18:1, and 18:0 were identified as molecular species of lyso-phosphatidylcholine.2. Oxidative stress identified by oxisterols as a marker(1) Alcoholic disorder in rat small intestine and anti-oxidative effect of daizeinAcute ethanol feeding induced accumulation of cholesterol hydroper-oxide and oxysterols in rat small intestine, suggesting that ethanol loading increased oxidative stress. Loading of Daisein in advance canceled such effects.(2) Fasting stress in ratOxidative stress due to fasting was stronger in the first day after fasting than in the second day in rat.(3) Oxidative stresss due to ischemic-reperfusion in turniquet-releaseThe enhanced oxidative stresss due to ischemic-reperfusion in turniquet-release mice were observed not only in the gastrocnemius muscles but also in the kidneys and liver.(4) Liver disorder by D-galactosamine and the effect of anti-oxidantD-galactosamine loading induced accumulation of cholesterol hydroperoxide and oxysterols in rat liver, brain and gastrocnemius muscles, suggesting that oxidative stress by D-galactosamine depend on organs or tissues.

1.氢过氧磷脂酰胆碱（PC 16：0 / 18：2-OOH）、氢氧化磷脂酰胆碱（PC 16：0 /18：2-OH，PC-OH）分子结构的鉴别通过LC/ESI-MS和LC/ESI-MS-MS在人血浆中鉴别了氢过氧磷脂酰胆碱（PC 16：0/18：2-OOH）、氢氧化磷脂酰胆碱（PC 16：0 / 18：2-OH，PC-OH）、环氧羟基-PC、氧代-PC、三羟基-PC。还鉴别了氢过氧溶血磷脂酰胆碱（18：2-OOH）和溶血磷脂酰胆碱氧化物（氧代-18：2）。18：2、20：4、16：0、18：1和18：0为溶血磷脂酰胆碱的分子种类。（1）大鼠小肠酒精性疾病及大豆黄酮的抗氧化作用急性乙醇喂养可诱导大鼠小肠胆固醇过氧化氢和氧化甾醇的积累，提示乙醇负荷增加了氧化应激。提前装载Daisein取消了这种效果。(2)大鼠禁食应激大鼠禁食后第1天的氧化应激强度高于第2天。(3)在释放姜黄素的小鼠中，由于缺血-再灌注引起的氧化应激增强不仅在腓肠肌中观察到，而且在肾脏和肝脏中观察到。(4)D-半乳糖胺引起的肝脏疾病和抗氧化剂D-半乳糖胺负荷的作用诱导了胆固醇氢过氧化物和氧化固醇在大鼠肝脏、脑和腓肠肌中的积累，表明D-半乳糖胺引起的氧化应激依赖于器官或组织。

项目成果

7-ketocholesterol存在下でのエタノールの肝細胞障害性.

7-酮胆固醇存在下乙醇的肝毒性。

• 发表时间: 2007
• 期刊: アルコールと医学生物学 27
• 作者: 浅野 水辺;他
• 通讯作者: 他

GC-MSによる死蝋中脂質の構造解析.

通过 GC-MS 对死蜡中的脂质进行结构分析。

• 发表时间: 2006
• 作者: 主田 英之;他
• 通讯作者: 他

Skeletal muscle and liver oxysterols during fasting and alcohol exposure

• DOI: 10.1016/j.metabol.2005.08.003
• 发表时间: 2006-01-01
• 期刊: METABOLISM-CLINICAL AND EXPERIMENTAL
• 影响因子: 9.8
• 作者: Adachi, J;Kudo, R;Preedy, VR
• 通讯作者: Preedy, VR

Analysis of phosphatidylcholine oxidation products in human plasma using quadrupole time-of-flight mass spectrometry

使用四极杆飞行时间质谱法分析人血浆中的磷脂酰胆碱氧化产物

• 发表时间: 2006
• 期刊: Kobe J Med Sci 52(5)
• 作者: Adachi;J;Asano;M;Yoshioka;N;Nushida;H;Ueno;Y
• 通讯作者: Y

Role of oxidative stress in paraquat poisoning (2)

氧化应激在百草枯中毒中的作用 (2)

• 发表时间: 2007
• 作者: Kurisaki;E;Hiraiwa;K;Adachi;J;Nakagawa;K;Ueno;Y
• 通讯作者: Y