Role of in glomerular injury

我们以前已经证明，抗粘附素分子，足糖萼蛋白结合的衔接蛋白和相互作用是重要的，以维持足细胞结构的独特组织。在这个研究项目中，我们关注足糖萼蛋白的糖基化改变，它应该影响肾小球粘附，表明严重肾脏疾病的标志物。我们研究了足细胞特异表达的糖转移酶，并进行了2-D SDS-PAGE检测足糖萼蛋白的糖基化改变。此外，我们测试了足细胞特异性mRNA的表达，是否新的基质材料，蜂窝状图案的膜提供了合适的足细胞培养的周长。结论：1.通过RT-PCR方法发现两种独特的糖转移酶（糖蛋白-N-乙酰半乳糖胺-3-13-半乳糖基转移酶，C1 Gal-t-1，UDP-G1 cNAc 2-差向异构酶，GNE）在足细胞中表达。足细胞特异性mRNA（nephrin、CD 2AP、podocalyxin）表达在蜂窝状膜上培养的足细胞中增加。扫描电镜观察在该膜上培养的多层细胞.我们还发现足细胞系中足糖萼蛋白和衔接蛋白（NHERF 2和ezrin）之间的相互作用与血管紧张素II浓度成比例地降低。由于技术上的问题，我们未能分析纯化的足糖萼蛋白的糖链。

We have previously demonstrated that an anti-adhesin molecule, podocalyxin binds to the adaptor proteins and that the interaction is important to maintain the unique organization of podocyte structure. In this research project, we focused on altered glycation of podocalyxin that should affect glomerular adhesion, indicating a marker of severe kidney diseases. We investigated what kind of sugar transferases are specifically expressed in podocyte and carried out 2-D SDS-PAGE to detect the altered glycation of podocalyxin. Also, we tested podocyte-specific mRNA expression whether novel material of substratum, honeycomb patterned film provides suitable circumference for podocyte culture.1. We found that two unique sugar transferases (glycoprotein-N-acetylgalactosamine-3-13-galactosyltransferase, C1Gal-t-1, UDP-G1cNAc 2-epimerase, GNE) are expressed in podocyte in vitro and in vivo by RT-PCR.2. Podocyte-specific mRNA (nephrin, CD2AP, podocalyxin) expression was increased in podocyte cultured on honeycomb patterned-film. Multi-layered cells cultured on this film were observed by scanning electron microscopy.3. We also found that the interaction between podocalyxin and adaptor proteins (NHERF2 and ezrin) in podocyte cell lines was reduced in proportion to the angiotensin II concentration.4. We failed to analyze sugar chains of purified podocalyxin, because to technical problems.