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Effect of nutritional state during fetal and neonatal period on the postnatal development of feeding regulatory system and onst of Life style related disease

胎儿及新生儿期营养状态对产后摄食调节系统发育及生活方式相关疾病发生的影响

基本信息

批准号：
18591173
负责人：
YAMAMOTO Yukiyo
金额：
$ 2.39万
依托单位：
University of Occupational and Environmental Health, Japan
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

1. Postnatal development of feeding regulatory system in the rat hypothalamusGalanin like peptide (GALP) mRNA was first detected in the arcuate nucleus (ARC) on day 8. GALP mRNA was gradually increased between day 8 and 14 and markedly increased between day 14 and 40, which is the weaning and pubertal period in rats. After day 40, there were no significant differences in GALP rnRNA. In contrast to GALP, Neuropeptide Y (NPY) and proopiomelanocortin (POMC) mRNAs were detected in the ARC from day 1 and lasted to day 60. There was no sexual dimorphism in GALP, NPY and POMC mRNAs during postnatal development. These results suggest that the development of GALP may be associated with developmental changes such as weaning, feeding and maturation of reproductive functions.2. Effect of milk deprivation during nelnatal period on the Postnatal development of feeding regulatory system in the rat hypothalamusWe examined the effect of the milk deprivation for 24h on GALP, NPY and POMC mRNA in pups. G … More ALP mRNA did not change by milk deprivation on day 9 and 15, while milk deprivation had a significant effect on NPY and POMC mRNA on day 15. These results suggest that the regulatory mechanism of GALP mRNA is different from that of the NPY and POMC genes during postnatal development.3. GALP gene expression in the neural lob of rat pituitary: Effect of postnatal development and milk deprivation.The GALP rnRNA was first detected in the PP at day 1 very weakly and gradually increased throughout postnatal development. Next, we examined the effect of the milk deprivation for twenty-four hours on the gene expression of the GALP in the PP during pre-weaning period. After 24h milk deprivation, the GALP gene expression was significantly increased at both postnatal day 9 and 15. Development of GALP, which increased during postnatal development, May be associated with its critical role in the maturation of neurosecretory function in the PP. Significant increase in the expression of the GALP gene after milk deprivation suggested that osmolality change induced by milk deprivation might be a stimulant to induce the expression of the GALP gene in the preweaning period.4. Effect of high fat diet during fetal and neonatal period on the postnatal development of feeding regulatory system in the rat hypothalamusMaternal HF-diet resulted in a significant increase in GALP gene expression on postnatal day 21. While there were no significant effects on postnatal day 40 and 60. The results indicate that maternal HF-diet can have organizational effects on the expression of GALP gene in the hypothalamus during postnatal development. The developmental effects observed in the present study suggest one possible mechanism by which fetal and neonatal exposure to HF diet produces long-lasting alterations and could play an important role in the abnormality in the appetite-regulating mechanism. Less

1.大鼠下丘脑摄食调节系统的生后发育第8天在弓状核（ARC）中首次检测到甘丙肽样肽（Galanin like peptide，GALP）mRNA。GALP mRNA在第8 ~ 14天逐渐增加，在第14 ~ 40天即断乳期和青春期显著增加。40天后，GALP mRNA无显著差异。与GALP相反，从第1天开始在ARC中检测到神经肽Y（NPY）和阿黑皮素原（POMC）mRNA，并持续至第60天。GALP、NPY和POMC mRNA在生后发育过程中无性别二型性。这些结果提示GALP的发生可能与断奶、摄食和生殖功能成熟等发育变化有关.本实验观察了新生期缺乳对大鼠下丘脑摄食调节系统发育的影响。G ...更多信息在第9天和第15天，牛奶剥夺对ALP mRNA没有影响，而在第15天，牛奶剥夺对NPY和POMC mRNA有显著影响。这些结果表明，GALP mRNA在生后发育过程中的调控机制与NPY和POMC基因不同. GALP mRNA在大鼠垂体神经叶中的表达：出生后发育和缺乳的影响GALP mRNA在出生后第1天在PP中首次检测到非常弱的表达，并在整个出生后发育过程中逐渐增加。接下来，我们检测了断奶前阶段禁乳24小时对PP中GALP基因表达的影响。在24小时的牛奶剥夺后，GALP基因的表达显着增加，在出生后第9和15天。GALP在出生后的发育过程中增加，可能与其在PP神经分泌功能成熟中的关键作用有关。断乳后GALP基因的表达显著增加，提示断乳引起的渗透压变化可能是诱导断奶前期GALP基因表达的一种刺激物.胎儿期和新生期高脂饮食对大鼠下丘脑摄食调节系统生后发育的影响母体高脂饮食导致生后21天GALP基因表达显著增加。而对生后40和60天无显著影响。结果表明，母体高脂饮食对下丘脑GALP基因表达具有组织效应。在本研究中观察到的发育影响表明，胎儿和新生儿暴露于HF饮食产生持久的改变，并可能在食欲调节机制异常中发挥重要作用的一种可能的机制。少