Postnatal development of the brain- adipose axis and its abnormality : as the background of the metabolic syndrome

为了开始了解Nesfatin-1在出生后发育中的生理作用，我们使用原位杂交组织化学方法检测了出生后第1天至第60天PVN、SON和ARC中Nesfatin-1基因表达的发育变化。Nesfatin-1的发育在出生后下丘脑核中表现出不同的模式，可能与其在出生后发育中的区域特异性作用有关。接下来，我们检测了断奶前24小时母体剥夺对Nesfatin-1基因表达的影响。缺奶24 h后，出生后第15天Nesfatin-1基因表达显著升高。断乳后Nesfatin-1基因表达显著增加，提示断乳引起的渗透压变化可能是诱导断奶前Nesfatin-1基因表达的兴奋剂。Nesfatin-1的发育可能与其在下丘脑-垂体轴神经分泌功能成熟中的关键作用有关。

To begin to understand the physiological role of Nesfatin-1 during postnatal development, we examined the developmental changes of the Nesfatin-1 gene expression in the PVN, SON and ARC during postnatal day 1 to day 60, using in situ hybridization histochemistry. Development of Nesfatin-1, which showed different patterns each hypothalamic nucleus during postnatal development, may be associated with its region-specific roles during postnatal development.Next, we examined the effect of the maternal deprivation for twenty-four hours on the gene expression of the Nesfatin-1 during pre-weaning period. After 24 h milk deprivation, the Nesfatin-1 gene expression was significantly increased at postnatal day 15. Significant increase in the expression of the Nesfatin-1 gene after milk deprivation suggested that osmolality change induced by milk deprivation might be a stimulant to induce the expression of the Nesfatin-1 gene in the preweaning period. Development of Nesfatin-1 may be associated with its critical role in the maturation of neurosecretory function in the hypothalamus-pituitary axis.