Postnatal development of the brain- adipose axis and its abnormality : as the background of the metabolic syndrome

脑-脂肪轴的出生后发育及其异常：代谢综合征的背景

• 批准号: 20591236
• 负责人: YAMAMOTO Yukiyo
• 金额: $ 2.91万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591236/
• 关键词: Nesfatin-1; 室傍核; 弓状核; 摂食調節; 生後発達; 脳脂肪細胞系; 視床下部; 視索上核; Galanin-like peptide; Neuromedin U; 下垂体後葉; 下垂体前葉; 浸透圧調節

To begin to understand the physiological role of Nesfatin-1 during postnatal development, we examined the developmental changes of the Nesfatin-1 gene expression in the PVN, SON and ARC during postnatal day 1 to day 60, using in situ hybridization histochemistry. Development of Nesfatin-1, which showed different patterns each hypothalamic nucleus during postnatal development, may be associated with its region-specific roles during postnatal development.Next, we examined the effect of the maternal deprivation for twenty-four hours on the gene expression of the Nesfatin-1 during pre-weaning period. After 24 h milk deprivation, the Nesfatin-1 gene expression was significantly increased at postnatal day 15. Significant increase in the expression of the Nesfatin-1 gene after milk deprivation suggested that osmolality change induced by milk deprivation might be a stimulant to induce the expression of the Nesfatin-1 gene in the preweaning period. Development of Nesfatin-1 may be associated with its critical role in the maturation of neurosecretory function in the hypothalamus-pituitary axis.

为了开始了解Nesfatin-1在出生后发育过程中的生理作用，我们采用原位杂交组织化学方法检测了出生后1 - 60天PVN、SON和ARC中Nesfatin-1基因表达的发育变化。Nesfatin-1在下丘脑各核团的发育过程中表现出不同的模式，这可能与其在下丘脑各核团发育过程中的区域特异性作用有关。24 h禁乳后，Nesfatin-1基因表达在出生后15天显著增加。断乳后Nesfatin-1基因的表达显著增加，提示断乳引起的渗透压变化可能是诱导断乳前期Nesfatin-1基因表达的一种刺激因素。Nesfatin-1的发育可能与其在下丘脑-垂体轴神经分泌功能成熟中的关键作用有关。

项目成果

Decreased plasma levels of brain-derived neurotrophic factor and its relationship with obesity-related metabolic complication, birth weight and plasma levels of adipocytokines in obese children

肥胖儿童血浆脑源性神经营养因子水平降低及其与肥胖相关代谢并发症、出生体重和血浆脂肪细胞因子水平的关系

• 发表时间: 2010
• 作者: Yamamoto Y;Araki S;Dobashi k;Gotoh M;Kusuhara K
• 通讯作者: Kusuhara K

Developmental changes of neuromedin U and galanin-like peptide gene expressions in the rat pituitary : an analysis by in situ hybridization histochemistry.

大鼠垂体神经调节素 U 和甘丙肽样肽基因表达的发育变化：原位杂交组织化学分析。

• 发表时间: 2010
• 作者: Kubo K;Yamamoto Y;Kawagoe R;Gotoh M;So M;Araki S;Kusuhara K.
• 通讯作者: Kusuhara K.

尿糖陽性で発見された幼児糖尿病の一例

尿糖阳性发现婴儿糖尿病一例

• 发表时间: 2009
• 作者: 河田泰定;荒木俊介;久保和泰;川越倫子;山本幸代;土橋一重;楠原浩一
• 通讯作者: 楠原浩一

長期臥床重症心身障害児における骨代謝マーカーおよび骨塩量の検討

长期卧床严重身心障碍儿童骨代谢标志物及骨矿物质含量检查

• 发表时间: 2009
• 作者: 石井雅宏;山本幸代;斉藤玲子;千手絢子;荒木俊介;久保和泰;川越倫子;河田泰定;下野昌幸;土橋一重
• 通讯作者: 土橋一重

腹腔鏡にて性腺摘出術を行ったX/XY混合性性腺異形成症の2例

腹腔镜性腺切除术治疗X/XY混合性性腺发育不良2例

• 发表时间: 2010
• 作者: 河田泰定;荒木俊介;久保和泰;川越倫子;山本幸代;楠原浩一
• 通讯作者: 楠原浩一