Membrane Protein Chip with Liposomes

带脂质体的膜蛋白芯片

• 批准号: 18310092
• 负责人: LEPIOUFLE Bruno
• 金额: $ 11.07万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310092/
• 关键词: Microfluidic Devices; Membrane Proteins; Quantitative Analysis; MEMS; Lab on a Chip; Lipid Bilavers; 膜タンパク質チップ; 脂質2重膜; リポソーム

Membrane proteins play very important roles in cells (e.g. recognition or transportation of molecules). They are also useful in various industrial fields, including next-generation diagnosis techniques, drug discovery, and highly sensitive ion-channel-based biosensors.In this research, we investigated a method toward membrane protein chips : an array of single-species-specific membrane proteins reconstituted into the surfaces of giant liposomes. In the method, we try to prepare monodisperse giant vesicles by (1) patterning lipid films on ITO substrates with a dry lift-off process and subsequently applying voltages between the substrates, or by (2)"blowing" a planar lipid bilayer and deforming it into vesicles (like blowing soap bubbles). We have also developed a simple method to make an array of the lipid bilayers on a microfluidic chip.We believe that these devices are useful for an efficient and rapid analysis of single-species-specific membrane proteins.

膜蛋白在细胞中发挥着非常重要的作用（例如分子的识别或运输）。它们也可用于各种工业领域，包括下一代诊断技术、药物发现和基于离子通道的高灵敏度生物传感器。在这项研究中，我们研究了一种膜蛋白芯片的方法：将一系列单物种特异性膜蛋白重组到巨型脂质体的表面。在该方法中，我们尝试通过以下方式制备单分散巨型囊泡：（1）通过干剥离工艺在 ITO 基底上形成脂质膜图案，然后在基底之间施加电压，或通过（2）“吹制”平面脂质双层并将其变形为囊泡（如吹肥皂泡）。我们还开发了一种简单的方法来在微流控芯片上制作脂质双层阵列。我们相信这些设备可用于高效、快速地分析单物种特异性膜蛋白。

项目成果

Lipid bilayer microarray for parallel recording of transmembrane ion currents

• DOI: 10.1021/ac7016635
• 发表时间: 2008-01-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Le Pioufle, Bruno;Suzuki, Hiroaki;Takeuchi, Shoji
• 通讯作者: Takeuchi, Shoji

Highly Parallelized Lipidic Bilayers Array for Ion Channel Recording

用于离子通道记录的高度并行脂质双层阵列

• 发表时间: 2007
• 作者: B. Le Pioufle;H. Suzuki;and S. Takeuchi
• 通讯作者: and S. Takeuchi

A parylene lift-off process with microfluidic channels for selective protein patterning

• DOI: 10.1088/0960-1317/17/3/011
• 发表时间: 2007-03-01
• 期刊: JOURNAL OF MICROMECHANICS AND MICROENGINEERING
• 影响因子: 2.3
• 作者: Atsuta, Kyoko;Suzuki, Hiroaki;Takeuchi, Shoji
• 通讯作者: Takeuchi, Shoji

A Parylene Lift-off Process with Microfluidic Channels for Selective Protein Patterning, Journal of Micromechanics and Microengineering

用于选择性蛋白质图案化的微流体通道聚对二甲苯剥离过程，《微机械与微工程杂志》

• 发表时间: 2007
• 期刊: Journal of Micromechanics and Microengineering vol.17
• 作者: K.Atsuta;H.Suzuki and S.Takeuchi
• 通讯作者: H.Suzuki and S.Takeuchi

Low Cost Microfabricated Silicon Chip for Membrane Protein Monitoring

用于膜蛋白监测的低成本微加工硅芯片

• 发表时间: 2006
• 期刊: microTAS2006
• 作者: B.Le Pioufle;C.Yamahata;K.Funakoshi;H.Suzuki;A.Tixier-Mita;H.Fujita;S.Takeuchi
• 通讯作者: S.Takeuchi