Membrane Protein Chip with Liposomes

膜蛋白在细胞中起着非常重要的作用（例如识别或运输分子）。在本研究中，我们研究了一种制作膜蛋白芯片的方法：将单一物种特异性的膜蛋白阵列重组到巨大脂质体的表面。在该方法中，我们试图通过（1）用干法剥离工艺在ITO基底上图案化脂质膜，随后在基底之间施加电压，或通过（2）“吹”平面脂质双层并使其变形为囊泡（如吹肥皂泡）来制备单分散巨囊泡。我们还开发了一种在微流控芯片上制作脂质双层阵列的简单方法，我们相信这些装置可用于高效、快速地分析单物种特异性膜蛋白。

Membrane proteins play very important roles in cells (e.g. recognition or transportation of molecules). They are also useful in various industrial fields, including next-generation diagnosis techniques, drug discovery, and highly sensitive ion-channel-based biosensors.In this research, we investigated a method toward membrane protein chips : an array of single-species-specific membrane proteins reconstituted into the surfaces of giant liposomes. In the method, we try to prepare monodisperse giant vesicles by (1) patterning lipid films on ITO substrates with a dry lift-off process and subsequently applying voltages between the substrates, or by (2)"blowing" a planar lipid bilayer and deforming it into vesicles (like blowing soap bubbles). We have also developed a simple method to make an array of the lipid bilayers on a microfluidic chip.We believe that these devices are useful for an efficient and rapid analysis of single-species-specific membrane proteins.