Precise Biopolymer Synthesis by Micro-space

微空间精密生物聚合物合成

• 批准号: 18310094
• 负责人: MIYAZAKI Masaya
• 金额: $ 10.73万
• 依托单位: National Institute of Advanced Industrial Science and Technology (2007)Kyushu University (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310094/
• 关键词: Microfluidics; Microreactor; Biopolymer; Polymerization; Laminar flow; Synthesis; Microdevice; Molecurlar conformation; 合成; 分子挙動; 酸無水物; 微少流体

In this project, we tried to develop microreaction system suitable for polymer synthesis and optimization of polymerization reaction. In linear polymer synthesis, such as amino acid polymerization and polypeptide synthesis reactions, it was found that mixing efficiency of reagents affect polymer properties. By applying efficient micromixer, we could synthesize peptide polymer with large molecular weight with narrow molecular weight distributions. This result might be useful for further development of microreactor for other linear polymer synthesis.Next, we examined poly-Lys dendrimer synthesis. However, this reaction was not successful because of difference in reactivity of amino groups at main -chain and side-chains.We also examined substitution of linear polymer to form the comb-like polymer. First, we examined behavior of biomolecules in microfluid. It was probed that microfluid structure affects the molecular conformation of biomolecules.This result means control of molecular structures of each main-and side chain polymers are important to improve reaction efficiency. Taking account of thiese, we designed microreaction system for PEGylation of poly-Lys.Overall, in this project, we revealed basic behavior of biomolecules in microfluid, and developed some efficient microreaction system for polymer synthesis.

本课题尝试开发适合于聚合物合成的微反应系统，优化聚合反应。在线性聚合物合成中，例如氨基酸聚合和多肽合成反应，发现试剂的混合效率影响聚合物的性质。利用高效的微混合器，可以合成分子量分布窄、分子量大的多肽聚合物。这一结果可能对其他线性高分子合成微反应器的进一步开发有一定的参考价值。然而，由于主链和侧链上氨基的反应性不同，该反应并不成功。首先，我们研究了微流体中生物分子的行为。探讨了微流体结构对生物分子构象的影响，这一结果意味着控制每个主链和侧链聚合物的分子结构对提高反应效率是非常重要的。基于此，我们设计了聚赖氨酸聚乙二醇化的微反应系统。总之，本课题揭示了生物分子在微流体中的基本行为，并开发了一些高效的微反应系统用于聚合物的合成。

项目成果

Protein refolding in microfluidic chip

微流控芯片中的蛋白质重折叠

• 发表时间: 2008
• 期刊: Peptide Science 2007
• 作者: Yamaguchi;H.;et. al.
• 通讯作者: et. al.

Protein Folding by Microfluid

微流体蛋白质折叠

• 发表时间: 2007
• 作者: Hiroshi;Yamaguchi;et. al.
• 通讯作者: et. al.

マイクロ流体中におけるDNAの2本鎖形成の熱力学的性質

微流体中 DNA 双链体形成的热力学特性

• 发表时间: 2007
• 作者: 山下健一;他
• 通讯作者: 他

Thermodynamic Properties of Duplex DNA in Microchamel Laminar Flow

Microchamel 层流中双链 DNA 的热力学性质

• 发表时间: 2007
• 期刊: ChemPhysChem 8
• 作者: Yamashita;K.;et. al.
• 通讯作者: et. al.

Changes in Molecular Conformation and Physicochemical Properties in Microfluid

微流体分子构象和理化性质的变化

• 发表时间: 2007
• 作者: Kenichi;Yamashita;et. al.
• 通讯作者: et. al.