Why does the autoantibody against cytokine incease in patients with idiopathic pulmonary alveolar proteinosis?

为什么特发性肺泡蛋白沉积症患者细胞因子自身抗体升高？

• 批准号: 18390240
• 负责人: NAKATA Koh
• 金额: $ 10.84万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390240/
• 关键词: pulmonary alveolar proteinosis; granulocyte macrophage colony stimulating factor; alveolar macrophage; lung surfactant; bronchoalveolar lavage; autoantibody; cytokine; autoimmune disease; ケモカイン; 原発性肺胞蛋白症; 免疫複合体

Recent studies have revealed that neutrophils from PAP patients had reduced basal functions such as phagocytosis, cell-adhesion, H_2O_2 production, and microbial killing (Uchida, et. Al.. NEJM.2007). In contrast, we found that some subsets of peripheral blood T and B cells were activated or excessively matured compared to normal controls. In CD4^+ cells, the number of naive T cells was reduced and the effecter-memory/naive ratio was up-regulated. Interestingly, small amount of CD4^+ cells were proliferating ex vivo without any stimulation. In B cells, CD38^+ activated cells were increased in both naive and memory B cell subset, whereas the expression of CD19 was reduced compared to the controls. Cells expressing CD138, a plasma cell marker, were significantly increased. Importantly, GM-CSF autoantibody was detected in the culture supernatants of peripheral blood mononuclear cells after 2 days incubation without any stimulation. These results suggested that some subsets of T and B cells were driven to be matured/activated and possibly promoted to produce the autoantibody.

最近的研究表明，PAP患者的中性粒细胞具有吞噬、细胞黏附、H_2O_2产生和微生物杀伤等基本功能。阿尔..。NEJM.2007)。相反，我们发现与正常对照组相比，外周血中T和B细胞的某些亚群被激活或过度成熟。在CD_4~+细胞中，幼稚T细胞数量减少，效应记忆/幼稚T细胞比值上调。有趣的是，少量的CD4^+细胞在没有任何刺激的情况下在体外增殖。在B细胞中，幼稚B细胞亚群和记忆性B细胞亚群的CD38~+活化细胞均高于对照组，而CD19的表达低于对照组。浆细胞标志物CD138的表达细胞显著增加。重要的是，在没有任何刺激的情况下，培养2天的外周血单个核细胞培养上清中可检测到GM-CSF自身抗体。这些结果表明，T和B细胞的某些亚群被驱动成熟/激活，并可能促进产生自身抗体。

项目成果

肺胞タンパク症のブレイクスルー

肺泡蛋白沉积症的突破

• 发表时间: 2007
• 作者: Hayashida M;et. al.;Yamada T;中田 光
• 通讯作者: 中田 光

今日の診断基準

今天的诊断标准

• 发表时间: 2007
• 作者: 綾田稔(代表);竹内薫;竹田誠;扇本真治;Luna Bhatta Sharma;石田博;田中美有;桑村充;小倉壽;児玉 浩子(分担)
• 通讯作者: 児玉 浩子(分担)

A survey of tuberculosis prevalence in Hanoi, Vietnam.

越南河内结核病患病率调查。

• 发表时间: 2007
• 期刊: Int J Tuberc Lung Dis. 11(5)
• 作者: Horie T;Lien LT;Tuan LA;Tuan PL;Sakurada S;Yanai H;Keicho N;Nakata K.
• 通讯作者: Nakata K.

Outbreak of Nocardia farcinica infection with the same pattern in randomly amplified polymorphic DNA analysis.

随机扩增多态性 DNA 分析中，猪粪诺卡氏菌感染的爆发具有相同的模式。

• 发表时间: 2006
• 期刊: J Hosp Infect. 2006 Apr;62(4) : 502-6. Epub 2006 Feb 17. 62(4)
• 作者: Kachi S;et al.
• 通讯作者: et al.

Pulmonary Mycobacterium avium complex infection:: association with NRAMP1 polymorphisms

• DOI: 10.1183/09031936.00042506
• 发表时间: 2007-07-01
• 期刊: EUROPEAN RESPIRATORY JOURNAL
• 影响因子: 24.3
• 作者: Tanaka, G.;Shojima, J.;Keicho, N.
• 通讯作者: Keicho, N.