Analysis of the periodontal natural immunity activation mechanism by Beta-defensin-2 production

Beta-defensin-2 产生对牙周自然免疫激活机制的分析

• 批准号: 21792137
• 负责人: TAGUCHI Yoichiro
• 金额: $ 2.83万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21792137/
• 关键词: ペプチド; 上皮細胞; 炎症; 歯周病治療; 歯周病; 歯肉; 炎症性メディエーター

Human β-defensin-2 (hBD-2) is one of antimicrobial peptides produced by epithelium cells after stimulation by microorganisms and inflammatory mediators. We reported that expression of hBD-2 mRNA in HGEC was P. gingivalis-dependently induced according to stimulation time and was connected with the initial stage of inflammatory responses after infection by pathogenic periodontal bacteria, with ultimately migration of neutrophils to infection sites. We investigated the difference between the expression of hBD-2 and the production of IL-8 in HGEC stimulated with various concentrations of P. gingivalis by RT-PCR and ELISA. Although the levels of hBD-2 mRNA and IL-8 production stimulated with P. gingivalis tended to increase with exposure time, the levels were not significantly changed by variations in concentration. These findings suggest that the expression of hBD-2 and IL-8 was observed in P. gingivalis-stimulated HGEC regardless of the severity of the inflammation.

人β-防御素-2（HBD-2）是微生物和炎症介质刺激后由上皮细胞产生的抗菌胡椒粉之一。我们报道说，HBD-2 mRNA在HGEC中的表达是根据刺激时间诱导的牙龈疟原虫，并与致病性牙周细菌感染后炎症反应的初始阶段有关，最终将中性粒细胞迁移到感染部位。我们研究了HBD-2的表达与RT-PCR和ELISA刺激的HGEC中IL-8的产生之间的差异。尽管牙龈疟原虫刺激的HBD-2 mRNA和IL-8产生水平往往随着暴露时间而增加，但浓度的变化并没有显着改变水平。这些发现表明，无论炎症的严重程度如何，HBD-2和IL-8的表达均在牙龈疟原虫刺激的HGEC中观察到。

项目成果

Effectiveness of calcium hydroxide on LPS-induced hard tissue resorption.

氢氧化钙对 LPS 诱导的硬组织吸收的有效性。

• 发表时间: 2010
• 期刊: Journal of Osaka Dental University 44
• 作者: Muneyasu Shida;Tadahiko Baba;Hirotaka Kon'i;Yoichiro Taguchi;Masatoshi Ueda;Hiroyuki Hayashi.
• 通讯作者: Hiroyuki Hayashi.

術審美再建歯周外科カラーアトラス第3版(第23章抜歯窩保存術第24章乳頭再建)(E.S.コーエン編)

审美重建牙周外科彩色图谱第3版（第23章拔牙槽保留第24章乳头重建）（E.S. Cohen编辑）

• 发表时间: 2009
• 作者: 鴨井久一;上田雅俊;田口洋一郎
• 通讯作者: 田口洋一郎

Double-immunostaining of p63 protein and cytokeratin 8 in pleomorphic adenoma.

多形性腺瘤中 p63 蛋白和细胞角蛋白 8 的双重免疫染色。

• 发表时间: 2009
• 期刊: Oral Medicine & Pathology 14(2)
• 作者: Masahiro Wato;Hirotaka Kon-i;Yoichiro Taguchi;Masatoshi Ueda;Ken-ichi Uobe;Kazuya Masuno;Kazuya Tominaga;Tetsunari Nishikawa;Akio Tanaka.
• 通讯作者: Akio Tanaka.

ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ由来合成ペプチドの有用性

牙釉质基质衍生物合成肽的实用性基于其对人牙龈上皮细胞的影响

• 发表时间: 2010
• 期刊: 日本歯科保存学雑誌 53
• 作者: 田口洋一郎;安井菜津希;富永和也;橋本典也;至田宗泰;玉置敏夫;北條博一;武田昭二;林宏行;田中昭男;上田雅俊
• 通讯作者: 上田雅俊

Effects of oligopeptide derived from enamel matrix derivative on rat bone marrow stromal cells

牙釉质基质衍生物寡肽对大鼠骨髓基质细胞的影响

• 发表时间: 2009
• 作者: Yoichiro Taguchi;Natsuki Yasui;Masatoshi Ueda et al.
• 通讯作者: Masatoshi Ueda et al.