Analysis of the periodontal natural immunity activation mechanism by Beta-defensin-2 production

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Human β-defensin-2 (hBD-2) is one of antimicrobial peptides produced by epithelium cells after stimulation by microorganisms and inflammatory mediators. We reported that expression of hBD-2 mRNA in HGEC was P. gingivalis-dependently induced according to stimulation time and was connected with the initial stage of inflammatory responses after infection by pathogenic periodontal bacteria, with ultimately migration of neutrophils to infection sites. We investigated the difference between the expression of hBD-2 and the production of IL-8 in HGEC stimulated with various concentrations of P. gingivalis by RT-PCR and ELISA. Although the levels of hBD-2 mRNA and IL-8 production stimulated with P. gingivalis tended to increase with exposure time, the levels were not significantly changed by variations in concentration. These findings suggest that the expression of hBD-2 and IL-8 was observed in P. gingivalis-stimulated HGEC regardless of the severity of the inflammation.