In vivo microvascular regeneration in the various conditions and molecular expressions and regulations

体内微血管再生的各种条件及分子表达与调控

• 批准号: 18591967
• 负责人: TANAKA Katsumi
• 金额: $ 2.35万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591967/
• 关键词: cytokine; optimal microvascular environment; clinical assessment; lower calf wound healing; sensory nerves; micro-angiogenesis; 基礎疾患; 人工真皮; 血管化腹壁皮弁; 間葉系幹細胞; 血流付加

Obesity, hypertension, atherosclerosis obliterans (ASO)and diabetes mellitus (DM) are increasing as co-morbidities increasing among those who are undertaking free flap surgeries and digital re-plantations. In these co-morbidities, there are remarkable damages in the endothelial cells of these blood vessels and thus induced hypoxia and the oxidized stresses and ischemic-reperfusion injury are highly concerned. Even macroscopically successful re-anastomoses may lead to thrombosis afterward. Thus, the optimal microvascular environment was investigated with low-sensory and low-blood flow models.In the microvascular models, after ligating the femoral vessels and severing the saphenous nerves, the epigastric fascial flap of which the nutrient vessels are the superficial epigastric artery and veins, covered the ligated-vessels and severed-nerves by wrapping. The human derived mesenchymal stem cells, together with basic fibroblast growth factor (bFGF)were injected through the nutrient artery (the superficial epigastric artery)and then incubated for 10 minutes by clamping after the wrapped fascial flap was attached to the defects. The analysis of the nerve conduction velocity and tissue blood flows was temporally investigated. The ipsi-lateral 4-mm full-thickness paw skin defect was also compared among groups temporally.Also, clinically the head-and-neck surgery and extremities are sometimes difficult for microvascular surgical reconstructions, because spaspms and the differences of the diameters of the vessels are often experienced. As an alternative reconstructive modalities, especially severely damaged lower legs in the elderly population, the artificial dermis with cytokine (bFGF)were effective in wound coverage. In the analysis of quality of wounds, there is much better scar quality in bFGF-treated wounds from the immediately post-wounding as well as shortening the wound healing time.

肥胖、高血压、动脉粥样硬化闭塞症（ASO）和糖尿病（DM）的发病率在接受免费皮瓣手术和数字再植入术的人群中不断增加。在这些合并症中，这些血管的内皮细胞都有明显的损伤，因此引起缺氧，氧化应激和缺血再灌注损伤受到高度关注。即使在宏观上成功的再吻合也可能导致血栓形成。因此，采用低感觉和低血流量模型研究最佳微血管环境。在微血管模型中，以腹壁浅动静脉为营养血管的腹壁筋膜瓣在结扎股血管和切断隐神经后包裹结扎的血管和切断的神经。将人源性间充质干细胞连同碱性成纤维细胞生长因子（bFGF）经营养动脉（腹壁浅动脉）注入，包裹的筋膜瓣附着于缺损处后，夹持培养10分钟。暂时性观察神经传导速度和组织血流量的变化。同时比较各组同侧4-mm全层爪皮缺损。此外，临床上头颈部和四肢的微血管手术重建有时也很困难，因为经常会出现痉挛和血管直径的差异。作为一种可选择的重建方式，特别是在老年人严重受损的下肢，细胞因子人工真皮（bFGF）在伤口覆盖方面是有效的。在创面质量分析中，bfgf处理的创面从伤后即刻开始瘢痕质量明显改善，创面愈合时间明显缩短。

项目成果

Extended sigmoid-shaped free jejunal patch for reconstruction of the oral base and pharynx after total glossectoray with laryngectomy

延长乙状结肠游离空肠补片用于全舌切除术后口腔基部和咽部重建

• 发表时间: 2006
• 作者: Anraku;K.;Tanaka;K.;Hirano;A
• 通讯作者: A

Combined surgical excision and radiation therapy for keloid treatment

• DOI: 10.1097/scs.0b013e3180de62a1
• 发表时间: 2007-09-01
• 期刊: JOURNAL OF CRANIOFACIAL SURGERY
• 影响因子: 0.9
• 作者: Akita, Sadanori;Akino, Kozo;Hirano, Akiyoshi
• 通讯作者: Hirano, Akiyoshi

Cranial Vault Distraction : Its Illusionary Effect and Limitation

颅穹分心：其幻觉效应和局限性

• 发表时间: 2006
• 期刊: Plast Reconstr Surg 117
• 作者: Yano H;Tanaka K;Sueyoshi O;Takahashi K;Hirata R;Hirano A.
• 通讯作者: Hirano A.

Versatility of cheek and cervicofacial flap for reconstruction of cheek defects

面颊和颈面部皮瓣修复面颊缺损的多功能性

• 发表时间: 2007
• 作者: Tanaka;K.;Hirano;A
• 通讯作者: A

Vascurazied tissue transfer for the reconstruction following the tumor resection of the head and neck cancer involving skull base

涉及颅底的头颈癌肿瘤切除后的血管组织移植重建

• 发表时间: 2007
• 作者: Tanaka;K.;Anraku;K.;Sakihama;N.;Matsuo;T.;Nagata;I.;Hirano;A
• 通讯作者: A