The receptor tyrosine kinase expression in the flap and the gene therapy

受体酪氨酸激酶在皮瓣中的表达及基因治疗

基本信息

  • 批准号:
    14571715
  • 负责人:
  • 金额:
    $ 1.86万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

In order to investigate the receptor tyrosine kinase profiles, each component involving in the flap viability, such as epidermal keratinocyte, endothelial cell, dermal fibroblast and fetal dermal fibroblast was at first tested on the cell-to-cell interaction. The fetal fibroblast (BALB-3T3, clone A3) is well regulated signal-transduction pathway via leukemia inhibitory factor (LIE') associated receptor and intracellular mechanism such as STAT3 tyrosine phosphorylatiorn. The permanently transfected mouse full-length LIF cDNA into the ALB-3T3, clone A3 and the cell is passaged. The vascular endothelial growth facto (VEGF) significantly increased the cell growth in the doseincrease 1,10,100ng, dependent-manner. The phosphorylated-Erk p42/p44 Mitogen-Associated Protein (MAP), which is highly related to the proliferation of LIF-mediated cell, is induced from 1 to 30 minutes after 10ng VEGF and from l to 60 minutes after 100ng VEGF stimulation in the serum-free medium condition.The modified … More Boyden dual chamber analyses were performed with interaction between the human mesenchymal stem cell (hMSC), which is a key role player of the ectodermal as well as mesenchymal lineage, and other, adult cell types such as epidermal keratinocyte, endothelial cell and dermal fibroblast. In 16-hour incubation with the hMSC in the top chamber and adult cells in the lower chamber demonstrated the significant cell migration in epidermal keratinocyte compared to endothelial cell and dermal fibroblast (345.0±61.50,36.2±14.45, 63.8±24.81, respectively, p<0.01). The monolayer co-culture of hMSC and epidermal keratinocyte demonstrated basal membrane-like protein production between the two cell types by ultrastructural analyses. The hMSC stimulated by basic fibroblast growth factor (bFGF) accelerated the skin defect together with artificial dermis template. These results may indicate the fetal fibroblast, which is gene-transfected of LIF cDNA, is useful in cell proliferation and further the mesenchymal stem cell may be a powerful tool for regenerative medicine. Less
为了研究受体酪氨酸激酶的特性,首先检测了与皮瓣存活有关的各个成分,如表皮角质形成细胞、内皮细胞、真皮成纤维细胞和胎儿真皮成纤维细胞之间的相互作用。胎儿成纤维细胞(BALB-3T3,克隆A3)是通过白血病抑制因子(Lie‘)相关受体和STAT3酪氨酸磷酸化等细胞内机制调控的信号转导通路。将小鼠LIF全长c DNA永久性地转入ALB-3T3,克隆A3,细胞传代。血管内皮细胞生长因子(VEGF)以剂量增加1、10、100 ng的方式显著促进细胞生长。在无血清培养条件下,与激光诱导因子介导的细胞增殖密切相关的磷酸化ERK p42/p44丝裂原相关蛋白(MAP)在10 ng血管内皮生长因子刺激后1~30分钟和L刺激100 ng血管内皮细胞生长因子刺激后60分钟被诱导。改良的…更多的Boyden双室分析是在人类间充质干细胞(HMSC)与其他成人细胞类型(如表皮角质形成细胞、内皮细胞和真皮成纤维细胞)之间的相互作用下进行的,hMSC是外胚层和间质谱系的关键角色。与上室和下室成体细胞共同孵育16h,表皮角质形成细胞较内皮细胞和真皮成纤维细胞迁移明显(分别为345.0±61.5 0,36.2±14.45,63.8±2 4.81,P<0.0 1)。超微结构分析显示,hMSC与表皮角质形成细胞单层共培养可见两种细胞间产生基底膜样蛋白。碱性成纤维细胞生长因子(BFGF)刺激的hMSC与人工真皮模板共同促进皮肤缺损的修复。这些结果表明,转LIF基因的胎儿成纤维细胞可用于细胞增殖,间充质干细胞可能成为再生医学的有力工具。较少

项目成果

期刊论文数量(49)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Akita S, Fukui M, Nakagawa H, Fujii T, Akino K: "Cranial bone defect healing is accelerated by mesenchymal stem cell by co-administration of bone morphogenetic protein-2 and basic fibroblast growth factor"Wound Repair Regen. 12. 254-261 (2004)
Akita S、Fukui M、Nakakawa H、Fujii T、Akino K:“间充质干细胞通过骨形态发生蛋白 2 和碱性成纤维细胞生长因子的共同施用加速颅骨缺损愈合”伤口修复再生。
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    0
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Daian T, Ohtsuru A, Rogounovitch T, Ishihara H, Hirano A, Akiyama-Uchida Y, Saenko V, Fujii T, Yamashita S.: "Overexpression of insulin-like growth factor-1 (IGF-I) receptor and the invasiveness of cultured keloidfibroblasts."J invest Dermatol.. 120. 956-
Daian T、Ohtsuru A、Rogounovitch T、Ishihara H、Hirano A、Akiyama-Uchida Y、Saenko V、Fujii T、Yamashita S.:“胰岛素样生长因子-1 (IGF-I) 受体的过度表达及其侵袭性
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    0
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Fukui M, Akita S, Akino K.: "Ectopic bone, formation facilitated by human mesenchymal stem cells and osteogenic cytokines via nutrient vessel injection in a nude rat model."Wound Rep Reg.. (in press).
Fukui M、Akita S、Akino K.:“通过在裸鼠模型中注射营养血管,人类间充质干细胞和成骨细胞因子促进异位骨的形成。”Wound Rep Reg..(出版中)。
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田中克己: "形成外科におけるマイクロサージェリーの現況"日本生殖外科学会雑誌. 15:1. 26-34 (2002)
Katsumi Tanaka:“整形外科显微外科的现状”日本生殖外科学会杂志15:1(2002)。
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Akino K, Mineta T, Fukui M, Fujii T, Akita S.: "Bone morphogenetic protein-2 regulates proliferation of human mesenchymal stem cells."Wound Rep Reg.. 11. 354-360 (2003)
Akino K、Mineta T、Fukui M、Fujii T、Akita S.:“骨形态发生蛋白 2 调节人类间充质干细胞的增殖。”Wound Rep Reg.. 11. 354-360 (2003)
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TANAKA Katsumi其他文献

Practice of adaptation games aimed at learning reasonable accommodation
旨在学习合理通融的适应游戏实践

TANAKA Katsumi的其他文献

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{{ truncateString('TANAKA Katsumi', 18)}}的其他基金

Preparation and evaluation of functional carbon materials on metal substrates deposited with hydrocarbon pyrolysis method
烃热解法沉积金属基底功能碳材料的制备及评价
  • 批准号:
    24560820
  • 财政年份:
    2012
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Web Search Intent Detection and Query Processing Based on Various Search Intents
基于各种搜索意图的Web搜索意图检测和查询处理
  • 批准号:
    24240013
  • 财政年份:
    2012
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study on the processes of thin film preparation using nano-sized metal oxides for environmental usage
环境利用纳米金属氧化物薄膜制备工艺研究
  • 批准号:
    21560742
  • 财政年份:
    2009
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
In vivo microvascular regeneration in the various conditions and molecular expressions and regulations
体内微血管再生的各种条件及分子表达与调控
  • 批准号:
    18591967
  • 财政年份:
    2006
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The preparation of semiconductor nanoparticles by PLA in water and the their evaluation as functional materials
PLA在水中制备半导体纳米粒子及其功能材料评价
  • 批准号:
    17560592
  • 财政年份:
    2005
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Study of p,n Control of room-temparature Photoluminescence Thin Films
室温光致发光薄膜的p,n控制研究
  • 批准号:
    15560584
  • 财政年份:
    2003
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Multi-modal Content Retrieval and Presentation and Broadcast Content Generation for Mobile Environment
移动环境的多模式内容检索和演示以及广播内容生成
  • 批准号:
    14208036
  • 财政年份:
    2002
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Biomolecular approach for the optimal flap surgery
最佳皮瓣手术的生物分子方法
  • 批准号:
    12671750
  • 财政年份:
    2000
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Discovery of Semantic Structures and Access Control in Distributed Hypermedia
分布式超媒体中语义结构的发现和访问控制
  • 批准号:
    12680416
  • 财政年份:
    2000
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Study of Luminescence Wavelength Control on Room Temperature Luminescent Materials by μm-Region Analysis
μm区分析室温发光材料发光波长控制的研究
  • 批准号:
    11650695
  • 财政年份:
    1999
  • 资助金额:
    $ 1.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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ICF:一种安全有效治疗慢性非感染性葡萄膜炎的新型双靶点基因疗法
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    MR/Z50385X/1
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基因治疗和手术切除治疗局灶性癫痫的纵向结构和认知功能成像及结果预测。
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    MR/X031039/1
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QbD 的下一代自动化和 PAT 实施以及细胞和基因治疗的增强方法
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    10087446
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Phase I/II clinical trial of autologous T cell gene therapy to treat X-linked lymphoproliferative disease (XLP)
自体T细胞基因疗法治疗X连锁淋巴增殖性疾病(XLP)的I/II期临床试验
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    MR/Y019458/1
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GeneT:葡萄牙中心的基因治疗 CoE
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    23K15643
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