Analysis of the locomotor circuit formation in zebrafish spinal cord

斑马鱼脊髓运动回路形成的分析

• 批准号: 19300118
• 负责人: HIGASHIJIMA Shin-ichi
• 金额: $ 12.31万
• 依托单位: National Institutes of Natural Sciences Okazaki Research Facilities
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300118/
• 关键词: ゼブラフィッシュ; 神経分化; 神経回路; トランスジェニック; 脊髄; 非対称分裂

The p2 progenitor domain in the ventral spinal cord gives rise to two interneuron subtypes, V2a and V2b. The mechanism as to how V2a and V2b neurons arise from a single progenitor domain was unknown. We showed in this study that p2 progenitors divide once to produce V2a/V2b neuron pairs, indicating that V2a and V2b neurons are generated by the asymmetric division of pair-producing progenitor cells. We also showed that Delta-Notch interactions between sister cells play a critical role in the final outcome of these asymmetric divisions. This mechanism for determining cell fate is similar to asymmetric divisions that occur during Drosophila neurogenesis, where ganglion mother cells divide once to produce distinct neurons. However, unlike in Drosophila, the divisional axes of p2 progenitors in zebrafish were not fixed. We report that the terminal division of pair-producing progenitor cells in vertebrate neurogenesis can reproducibly produce two distinct neurons through a mechanism that may not depend on the orientation of the division axis.

腹侧脊髓中的 p2 祖细胞结构域产生两种中间神经元亚型：V2a 和 V2b。 V2a 和 V2b 神经元如何从单个祖细胞结构域产生的机制尚不清楚。我们在这项研究中表明，p2 祖细胞分裂一次产生 V2a/V2b 神经元对，表明 V2a 和 V2b 神经元是由成对祖细胞的不对称分裂产生的。我们还表明，姐妹细胞之间的 Delta-Notch 相互作用在这些不对称分裂的最终结果中发挥着关键作用。这种决定细胞命运的机制类似于果蝇神经发生过程中发生的不对称分裂，其中神经节母细胞分裂一次以产生不同的神经元。然而，与果蝇不同，斑马鱼 p2 祖细胞的分裂轴不是固定的。我们报告说，脊椎动物神经发生中成对祖细胞的终末分裂可以通过一种可能不依赖于分裂轴方向的机制可重复地产生两个不同的神经元。

项目成果

A topographic map of recruitment in spinal cord

• DOI: 10.1038/nature05588
• 发表时间: 2007-03-01
• 期刊: NATURE
• 影响因子: 64.8
• 作者: McLean, David L.;Fan, Jingyi;Fetcho, Joseph R.
• 通讯作者: Fetcho, Joseph R.

From the Olfactory Bulb to Higher Brain Centers: Genetic Visualization of Secondary Olfactory Pathways in Zebrafish

• DOI: 10.1523/jneurosci.0118-09.2009
• 发表时间: 2009-04-15
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Miyasaka, Nobuhiko;Morimoto, Kozo;Yoshihara, Yoshihiro
• 通讯作者: Yoshihara, Yoshihiro

Functional Role of a Specialized Class of Spinal Commissural Inhibitory Neurons during Fast Escapes in Zebrafish

• DOI: 10.1523/jneurosci.0801-09.2009
• 发表时间: 2009-05-27
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Satou, Chie;Kimura, Yukiko;Higashijima, Shin-ichi
• 通讯作者: Higashijima, Shin-ichi

Illuminating cell-cycle progression in the developing zebrafish embryo

• DOI: 10.1073/pnas.0906464106
• 发表时间: 2009-12-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Sugiyama, Mayu;Sakaue-Sawano, Asako;Miyawaki, Atsushi
• 通讯作者: Miyawaki, Atsushi

Proneural gene-linked neurogenesis in zebrafish cerebellum

• DOI: 10.1016/j.ydbio.2010.03.024
• 发表时间: 2010-07-01
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Kani, Shuichi;Bae, Young-Ki;Hibi, Masahiko
• 通讯作者: Hibi, Masahiko