Analysis of the locomotor circuit formation in zebrafish spinal cord

斑马鱼脊髓运动回路形成的分析

• 批准号: 19300118
• 负责人: HIGASHIJIMA Shin-ichi
• 金额: $ 12.31万
• 依托单位: National Institutes of Natural Sciences Okazaki Research Facilities
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300118/
• 关键词: ゼブラフィッシュ; 神経分化; 神経回路; トランスジェニック; 脊髄; 非対称分裂

The p2 progenitor domain in the ventral spinal cord gives rise to two interneuron subtypes, V2a and V2b. The mechanism as to how V2a and V2b neurons arise from a single progenitor domain was unknown. We showed in this study that p2 progenitors divide once to produce V2a/V2b neuron pairs, indicating that V2a and V2b neurons are generated by the asymmetric division of pair-producing progenitor cells. We also showed that Delta-Notch interactions between sister cells play a critical role in the final outcome of these asymmetric divisions. This mechanism for determining cell fate is similar to asymmetric divisions that occur during Drosophila neurogenesis, where ganglion mother cells divide once to produce distinct neurons. However, unlike in Drosophila, the divisional axes of p2 progenitors in zebrafish were not fixed. We report that the terminal division of pair-producing progenitor cells in vertebrate neurogenesis can reproducibly produce two distinct neurons through a mechanism that may not depend on the orientation of the division axis.

腹侧脊髓中的P2祖细胞结构域可导致两个间神经元亚型V2A和V2B。关于V2A和V2B神经元如何由单个祖细胞结构域产生的机制尚不清楚。我们在这项研究中表明，P2祖细胞曾经分裂以产生V2A/V2B神经元对，表明V2A和V2B神经元是由成对产生祖细胞的不对称分裂产生的。我们还表明，姊妹细胞之间的三角核相互作用在这些不对称分裂的最终结果中起着关键作用。确定细胞命运的这种机制类似于果蝇神经发生过程中发生的不对称分裂，那里神经节母细胞一次分裂以产生不同的神经元。但是，与果蝇不同，斑马鱼中P2祖细胞的部门轴未固定。我们报告说，脊椎动物神经发生中成对产生的祖细胞的末端分裂可以通过可能不取决于分裂轴的方向的机制可重复产生两个不同的神经元。

项目成果

Illuminating cell-cycle progression in the developing zebrafish embryo

• DOI: 10.1073/pnas.0906464106
• 发表时间: 2009-12-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Sugiyama, Mayu;Sakaue-Sawano, Asako;Miyawaki, Atsushi
• 通讯作者: Miyawaki, Atsushi

Functional Role of a Specialized Class of Spinal Commissural Inhibitory Neurons during Fast Escapes in Zebrafish

• DOI: 10.1523/jneurosci.0801-09.2009
• 发表时间: 2009-05-27
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Satou, Chie;Kimura, Yukiko;Higashijima, Shin-ichi
• 通讯作者: Higashijima, Shin-ichi

Zebrafish and motor control over the last decade

• DOI: 10.1016/j.brainresrev.2007.06.018
• 发表时间: 2008-01-01
• 期刊: BRAIN RESEARCH REVIEWS
• 作者: Fetcho, Joseph R.;Higashijima, Shin-ichi;McLean, David L.
• 通讯作者: McLean, David L.

Vsx2 in the zebrafish retina: restricted lineages through depression.

斑马鱼视网膜中的 Vsx2：通过抑郁限制谱系。

• 发表时间: 2009
• 期刊: Neural Development 4
• 作者: Vitorino;M.;Jusf;P.R.;Maurus;D.;Kimura;Y.;Higashijima;S.;Harris;W.A.
• 通讯作者: W.A.

Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish.

• DOI: 10.1016/j.ydbio.2010.02.017
• 发表时间: 2010-04
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: H. Wada;A. Ghysen;Chie Satou;S. Higashijima;K. Kawakami;S. Hamaguchi;M. Sakaizumi