Establishment of microscopic analysis method of stress and strain fields in the biological tissues for unified understanding of mechanical adaptation mechanism from cells to tissues

建立生物组织应力应变场显微分析方法，统一认识细胞到组织的力学适应机制

• 批准号: 19300157
• 负责人: MATSUMOTO Takeo
• 金额: $ 12.23万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300157/
• 关键词: バイオメカニクス; 応力・ひずみ解析; 細胞・組織; 循環器・高血圧; 機械材料・材料化学; 生物・生体工学; 機械材料・材料力学

Biological tissues change their shape and mechanical properties adaptively in response to their mechanical environment. This response is caused by the cells in the tissue, which actively remodel the surrounding tissues in response to the force applied to them. As the first step to elucidate this mechanism, we have established a method to measure microscopic deformation of the cells during macroscopic tissue deformation and to estimate the force field in the tissue. It was found that 1) even a simple stretch of porcine thoracic aorta caused shear and rotation at a cellular level, i.e., microscopic deformation was completely different from macroscopic deformation ; 2) microscopic stress caused by the stretch was not uniform and could vary by a factor of 2 or more depending on the locations.

生物组织根据其机械环境适应性地改变其形状和机械特性。这种反应是由组织中的细胞引起的，这些细胞响应施加在其上的力而主动重塑周围组织。作为阐明这一机制的第一步，我们建立了一种方法来测量宏观组织变形期间细胞的微观变形并估计组织中的力场。结果发现：1）即使是猪胸主动脉的简单拉伸也会引起细胞水平的剪切和旋转，即微观变形与宏观变形完全不同； 2) 拉伸引起的微观应力不均匀，并且根据位置的不同可能会变化 2 倍或更多。

项目成果

細胞把持・回転観察装置

细胞夹持/旋转观察装置

• 发表时间: 2008

Mechanics of wrinkle formation : Micromechanical analysis of skin deformation during wrinkle formation in UV-irradiated mice

皱纹形成机制：紫外线照射小鼠皱纹形成过程中皮肤变形的微观力学分析

• 发表时间: 2010
• 期刊: Skin Res Technol
• 影响因子: 2.2
• 作者: Nakamura T;Phuong THN;Hori E;Urakawa S;Uwano T;Hamashima T;Ishii Y;Matsushima T;Ono T;Sasahara M;Nishijo H.;Matsumoto T
• 通讯作者: Matsumoto T

力学的視点から眺めた血管とリモデリング, Fireside seminar「メカノストレスによる血管内皮機能障害と血管リモデリング」

从机械角度看血管和重塑，炉边研讨会“机械应力引起的血管内皮功能障碍和血管重塑”

• 发表时间: 2010
• 作者: 長谷川克也;小笠原康夫;山本希美子;Katsuya Hasegawa;山本希美子;松本健郎
• 通讯作者: 松本健郎

Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate

• DOI: 10.1152/ajpcell.00098.2008
• 发表时间: 2008-12-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
• 影响因子: 5.5
• 作者: Nagayama, Kazuaki;Matsumoto, Takeo
• 通讯作者: Matsumoto, Takeo

Mechanical analysis of the aortic wall considering microscopic heterogeneity

考虑微观异质性的主动脉壁力学分析

• 发表时间: 2007
• 作者: T. Sugiura;M. Nakao;T. Sato and K. Minato;Nagayama K;Tamura A;Nagayama K;Nagayama K;松本 健郎;Matsumoto T;Yang YF;Nagayama K;Matsumoto T;Matsumoto T;Matsumoto T
• 通讯作者: Matsumoto T