Measurement of mechanical properties of cells based on their internal structures

根据细胞内部结构测量细胞的机械性能

• 批准号: 15086209
• 负责人: MATSUMOTO Takeo
• 金额: $ 25.6万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15086209/
• 关键词: Biomechanics; Cell mechanics; Cytoskeleton; Organelle; Vasuclar Smooth Muscle Cells; Mechanical Adaptation

Relationship linking mechanical properties of cells and their cytoskeletons are important in mechanotransduction. In this study, we measured quasi-static tensile properties and stress relaxation responses mainly of smooth muscle cells (SMCs) freshly isolated from aortic walls (FSMCs) and those cultured following isolation (CSMCs) with micro tensile testers to know the effects of the disruption of actin filaments (AF) or microtubules (MT). Their 3D microstructures were observed with a cell rotation system. Following results were obtained:1. FSMCs were stiffer in their longitudinal direction in which AFs align, and the stiffness of CSMCs decreased by 60% following AF disruption, indicating that AFs play dominant roles in their tensile properties.2. Stress relaxation response of CSMCs could be expressed with the combination of fast exponential decay with a time constant of〜1 min and slow decay with a time constant of 〜1 h, and the AF disruption caused significant change in the slow decay.3. We established a method to stretch cells maintaining their shape on substrate and found that the stiffness of CSMCs decreased by 70 and 30% following AF and MT disruption, respectively. MTs may play significant roles in the tensile properties of SMCs.4. We successfully observed with the cell rotation system that AFs aligned helically in the FSMCs and that nucleus of CSMCs buckled when the cells were detached from substrate and shrank.5. Local stiffness measured with an atomic force microscope increased following MT disruption in CSMCs. Whole-cell stiffness of cultured fibroblasts did not change following MT disruption, but decreased significantly by〜75% following AF disruption.These results indicate that not only AFs but also MTs have significant effects on cell mechanical properties, but the degree of contribution of MTs depends on the cell types and mode of deformation.

细胞及其细胞骨架的机械特性之间的关系在机械转导中很重要。在本研究中，我们主要测量了从主动脉壁新鲜分离的平滑肌细胞（FSMC）和使用微拉伸测试仪分离后培养的平滑肌细胞（CSMC）的准静态拉伸特性和应力松弛反应，以了解肌动蛋白丝（AF）或微管（MT）破坏的影响。使用细胞旋转系统观察它们的 3D 微观结构。得到如下结果： 1． FSMCs在AFs排列的纵向方向上较硬，并且AF破坏后CSMCs的刚度下降了60%，表明AFs在其拉伸性能中起主导作用。2． CSMCs的应力松弛响应可以用时间常数~1 min的快速指数衰减和时间常数~1 h的慢速衰减相结合来表达，并且AF破坏引起慢速衰减的显着变化。 3．我们建立了一种拉伸细胞以保持其在基底上的形状的方法，发现在 AF 和 MT 破坏后，CSMC 的硬度分别降低了 70% 和 30%。 MTs可能在SMCs的拉伸性能中发挥重要作用。4．通过细胞旋转系统，我们成功地观察到AFs在FSMCs中螺旋排列，并且当细胞脱离基质并收缩时，CSMCs的细胞核发生弯曲。5． CSMC 中 MT 破坏后，用原子力显微镜测量的局部刚度增加。培养的成纤维细胞的全细胞硬度在MT破坏后没有变化，但在AF破坏后显着下降约75%。这些结果表明，不仅AFs而且MTs对细胞力学性能都有显着影响，但MTs的贡献程度取决于细胞类型和变形模式。

项目成果

Tensile properties of fibroblasts and vascular smooth muscle cells

成纤维细胞和血管平滑肌细胞的拉伸特性

• 发表时间: 2003
• 期刊: Biorheology 40
• 作者: S.Taniguchi;5;Miyazaki H
• 通讯作者: Miyazaki H

Biomechanics at Micro- and Nanoscale Levels, vol. IV(分担執筆)

微米和纳米级生物力学，第四卷（撰稿人）

• 发表时间: 2007
• 作者: Wada H

細胞用二軸繰返引張機構の開発とこれを用いた血管内皮細胞の力学応答の解析

细胞双轴循环张力机制的开发及利用该机制分析血管内皮细胞的机械响应

• 发表时间: 2004
• 期刊: 日本機械学会論文集A編 70・694
• 作者: Nagayama K;Matsumoto T;長山 和亮;中垣 将
• 通讯作者: 中垣 将

血管壁の実験バイオメカニクス : マクロからミクロへ

血管壁实验生物力学：从宏观到微观

• 发表时间: 2006
• 期刊: 脈管学 46・6
• 作者: K.Matsuoka;et. al.;Hiromichi NAKAHARA;Kazuki HODA;Kazuki HODA;Kazuki HODA et al.;Hiromichi NAKAHARA et al.;O.Shibata;池田 裕里子;中原 広道;O.Shibata;中原 広道;中原 広道;池田 裕里子;Y.Moroi;O.Shibata;H.Nakahara;H.Nakahara;O.Shibata;H.Nakahara;Y.Moroi;Y.Moroi;Y.Moroi;柴田 攻;池田 裕里子;中原 広道;H.Nakahara;O.Shibata;Yuriko Ikeda;H.Nakahara;Nagayama K;松本 健郎
• 通讯作者: 松本 健郎

松本 健郎: "平滑筋収縮に伴う家兎動脈壁内ひずみ分布の変化-弾性動脈と筋性動脈の比較-"日本機械学会論文集A編. 69・677. 37-42 (2003)

Takeo Matsumoto：“平滑肌收缩引起的兔动脉壁内应变分布的变化 - 弹性动脉和肌性动脉的比较”日本机械工程师学会会议录，A 版 69, 677. 37-42 (2003)。