Localization of transmembrane receptors and functional and structural compartentalization of the cytoplasmic membrane

点击翻译按钮获取中文摘要

The chemoreceptors and the histidine kinases of Escherichia coli as well as protein components of chemotaxis-related signaling systems were analyzed for their localization to obtain several important results concerning the mechanisms underlying localization and its control of transmembrane sensors. Movements of the chemoreceptor through the membrane were analyzed to find that they are restricted by actin-like cytoskeleton or structures associated with it. The chemoreceptor was labeled with FlAsH and HaloTag to establish new systems for localization analyses. These results shed new light on the localization/function of transmembrane sensors and the architecture of the cytoplasmic membrane in prokaryotic cells.