Development of autologous bone marrow transplantation therapy using hematopoietic stem cells in dogs

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To advance antineoplastic therapies for lymphohematopoietic tumors that are common in dogs, we developed a series of systems for autologous bone marrow transplantation. Optimal conditions for bone marrow cell collection, hematopoietic cell separation, and their cryopreservation were obtained. Next, hematopoietic stem cells resistant to antineoplastic agents were generated by a gene transfer method. Finally, we developed a higly sensitive quantitative monitoring system of minimal residual disease to assess the treatment efficacy. The present study provided a basis for the development of antineoplastic therapies with excellent treatment outcomes.