Usefulness of tumor markers in veterinary medicine

肿瘤标志物在兽医学中的用途

• 批准号: 12460138
• 负责人: TSUJIMOTO Hajime
• 金额: $ 10.11万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460138/
• 关键词: Tumor marker; Telomere; Telomerase; Multidrug resistance; MDM2; Feline leukemia virus; Clonarity; P-glycoprotein; P糖蛋白質; イヌ; DNAワクチン; Cry j 1; T細胞エピトープ; アトピー性皮膚炎; 犬; 猫; P53; P糖蛋白

In order to show the usefulness of tumor markers in veterinary medicine, we measured a variety of tumor markers in dogs and cats with neoplastic diseases in small animal practice. During the 2 years for the present study, we investigated the usefulness of tumor markers classified into 6 categories : (1) serum (plasma) tumor marker, (2) intracellular telomerase and telomerase reverse transcriptase, (3) expression of P-53 and MDM2 and mutation of p53 gene, (4) cell clonarity, (5) chromosome abberation, and (6) P-glycoprotein (P-gp) associated with multidrug resistance. Major results of the present study are as follows.1.We measured the telomere length and intracellular telomerase activity in canine mammary tumors and found that telomerase activity could be used as a tumor marker.2.We examined the sequence of p53 gene in naturally occurring tumors in dogs and cats and found that p53 gene was mutated in approximately 50% of the dog tumors, therefore, found that mutation of p53 gene could b … More e used as a tumor marker in dogs. Moreover, a large number of dog tumors were shown to have an overexpression of MDM2 (a protein inhibiting P-53) which could be a candidate of new tumor marker.3.Southern blot analysis using exogenous feline leukemia virus (FeLV) was carried out in the peripheral blood samples and biopsy specimens of tumors, bone marrows and lymph modes. As a result, monoclonal or oligoclonal cell population was detected in neoplastic deseases (leukemia and myelodysplastic syndrome, MDS) and it was found that the cell clonality could be used as a new tumor marker in FeLV-infected cats.4.Nucleotide sequences of canine and feline mdr (multidrug resistance) genes that encodes P-gp were determined. Expressions of mdr RNA and P-gp protein were examined by RT-PCR and immunohistochemistry using a monoclonal antibody directed P-gp (C219), respectively, in tumor cells. By the treatment with antineoplastic drugs in conjunction with P-gp inhibitor, response to the chemotherapy was shown to be improved. Less

为了显示肿瘤标记在兽医医学中的有用性，我们测量了小动物练习中患有肿瘤疾病的狗和猫中的各种肿瘤标记。在本研究的两年中，我们研究了分类为6种的肿瘤标记物的有用性：（1）血清（血浆）肿瘤标记物，（2）细胞内端粒酶和端粒酶逆转录酶，（3）p-53和Mdm2的表达和MDM2的表达以及MDM2和MDM2和MDM2和P53基因，（4）Cell Clonarity，（4）Cell andos（4）Chronarity，（4）Chronarity，（5）chromy（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5） P-糖蛋白（P-GP）与多药电阻有关。 Major results of the present study are as follows.1.We measured the telomere length and intracellular telomerase activity in canine mammary tumors and found that telomerase activity could be used as a tumor marker.2.We examined the sequence of p53 gene in naturally occurring tumors in dogs and cats and found that p53 gene was mutated in Approximately 50% of the dog tumors, therefore, found that mutation of p53 gene could b …更多的E用作狗的肿瘤标记。 Moreover, a large number of dog tumors were shown to have an overexpression of MDM2 (a protein inhibiting P-53) which could be a candidate of new tumor marker.3.Southern blot analysis using exogenous feline leukemia virus (FeLV) was carried out in the peripheral blood samples and biopsy specimens of tumors, bone Marrows and lymph modes. As a result, monoclonal or oligoclonal cell population was detected in neoplastic deseases (leukemia and myelodysplastic syndrome, MDS) and it was found that the cell clonality could be used as a new tumor marker in FeLV-infected cats.4.Nucleotide sequences of canine and feline mdr (multidrug resistance) genes that encodes P-gp were determined.通过RT-PCR和免疫组织化学研究了MDR RNA和P-gp蛋白的表达，分别使用肿瘤细胞中的单克隆抗体（C219）进行了免疫组织化学。通过与P-gp抑制剂结合使用抗塑性药物的治疗，对化学疗法的反应得到了改善。较少的

项目成果

Masuda, K., et al.: "In vivo and in vitro tests showing sensitization to Japanese cedar (Cryptomeria japonica)pollen in atopic dogs"J.Vet.Med.Sci.. 62. 995-1000 (2000)

Masuda, K., et al.：“体内和体外测试显示特应性狗对日本柳杉 (Cryptomeria japonica) 花粉的敏感性”J.Vet.Med.Sci.. 62. 995-1000 (2000)

Masuda, K., et al.: "Seasonal rhinitis in a cat sensitized to Japanese cedar(Cryptomeria japonica)pollen"J.Vet.Med.Sci.. 63. 79-81 (2001)

Masuda, K., et al.：“对日本柳杉（Cryptomeria japonica）花粉敏感的猫的季节性鼻炎”J.Vet.Med.Sci.. 63. 79-81 (2001)

Hisasue, M., et al.: "Clonality analysis of various hematopoietic disorders in cats naturally infected with feline leukemia virus."J.Vet.Med.Sci.. 62. 1059-1065 (2000)

Hisasue, M., 等人：“自然感染猫白血病病毒的猫中各种造血系统疾病的克隆性分析。”J.Vet.Med.Sci.. 62. 1059-1065 (2000)

Yamashita, K., et al.: "Experimental sensitization with Japanese cedar pollen in dogs"J.Vet.Med.Sci.. 62. 1223-1225 (2000)

Yamashita, K., et al.：“狗中日本柳杉花粉的实验致敏”J.Vet.Med.Sci.. 62. 1223-1225 (2000)

Setoguchi, A., et al.: "Results of hyperamplification of centrosomes in naturally developing tumors of dogs."Am.J.Vet.Res.. 62. 1134-1141 (2001)

Setoguchi, A., et al.：“狗自然发育肿瘤中中心体过度扩增的结果。”Am.J.Vet.Res.. 62. 1134-1141 (2001)