Fundamental Research on the Cat Genome Analysis

猫基因组分析的基础研究

• 批准号: 10460138
• 负责人: TSUJIMOTO Hajime
• 金额: $ 2.62万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460138/
• 关键词: Cat; Genome analysis; FISH analysis; Oncogene; Tumor suppressor gene; Immunoglobulin gene; T-cell receptor gene; Lymphoma; 癌抑制遺伝子; 免疫グログリン遺伝子; in situ ハイブリダイゼーション; 腫瘍; p53

In the present studies on the cat genome analysis, we carried out fluorescence in situ hybridization (FISH) analysis to investigate a number of genes associated with tumorigenesis in the cat.[Oncogene] By FISH analysis, myc gene was assigned to cat chromosome F2q21.2. Fit-1 region, associated with the tumorigenesis in cat lymphomas, was shown to be present in a locus closely linked to that of myb gene on cat chromosome B2. [Tumor suppressor and cyclin dependent kinase (Cdk) in hibitor genes] FISH analysis indicated that p53 gene, p15MTS2/p16MTSI gene, p21 WAFI gene, and p27kipl gene were assigned to cat chromosome E1p14-p13, D4q12, B2, and B4, respectively. [Immunoglobulin and T-cell receptor genes] Ig heavy chain gene, Ig light (lambda) chain gene, and TCR γ chain gene were assigned to cat chromosome B3q26, D3p12-p11, and A2q12-q13, respectively. From these chromosome mapping studies, loci of a number of genes associated with the tumorigenesis in cat lymphoma and leukemia could be identified on cat genome.Moreover, from a series of chromosome analysis of cat lymphoma cells including clinical specimens, we could identify a chromosomal translocation between F2 and B3 chromosomes in cat 3201B T-lymphoma cell line. FISH analysis of this cell line revealed that myc gene on the long arm of F2 chromosome and Ig heavy chain gene on the long arm of B3 chromosome were shown to be adjacent on the on the same chromosome.The present studies were carried out by cytogenetic approaches in cats. The results of the studies will provide fundamental informations on the advanced researches on the tumorigenesis not only in cats but also in humans.

在目前的猫基因组分析研究中，我们进行了荧光原位杂交（FISH）分析，以调查一些基因与肿瘤发生的猫。【癌基因】通过FISH分析，myc基因定位于猫染色体F2q21.2。FIT-1区域与猫淋巴瘤的发生有关，存在于猫染色体B2上与myb基因紧密连锁的位点。[肿瘤抑制基因和细胞周期蛋白依赖激酶（Cdk）抑制基因的研究]荧光原位杂交（FISH）结果表明，p53基因、p15 MTS 2/p16 MTSI基因、p21 WAFI基因和p27 kipl基因分别位于猫染色体E1 p14-p13、D4 q12、B2和B4上。[免疫球蛋白和T细胞受体基因] IG重链基因、IG轻（λ）链基因和TCR γ链基因分别被分配到猫染色体B3 q26、D3 p12-p11和A2 q12-q13。通过对猫淋巴瘤和白血病的染色体定位研究，在猫基因组上发现了一些与猫淋巴瘤和白血病发生相关的基因位点，并通过对猫淋巴瘤细胞包括临床标本的染色体分析，发现猫3201 B T淋巴瘤细胞系存在F2和B3染色体易位。对该细胞系的FISH分析表明，F2染色体长臂上的myc基因和B3染色体长臂上的IG重链基因位于同一条染色体上。本研究结果将为进一步研究猫和人的肿瘤发生机制提供基础资料。

项目成果

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Endo, Y. 等人：“CC 趋化因子 cDNA 的分子克隆。”

Mizuno, T.et al.: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol. 65. 161-172 (1998)

Mizuno, T.等人：“猫科动物 Fas 抗原和 Fas 配体 cDNA 的分子克隆。”

Cho, K.W.et al.: "Assignment of the cat p53 tumor suppressor gene (TP53) to cat chromosome E1p14-p13 by fluorescence in situ hybridization."Cytogent.Cell Genet.. 79. 145-146 (1998)

Cho, K.W.等人：“通过荧光原位杂交将猫 p53 肿瘤抑制基因 (TP53) 分配给猫染色体 E1p14-p13。”Cytogent.Cell Genet.. 79. 145-146 (1998)

Okai, Y.et al.: "Molecular analysis of multidrug resistance in lymphoma cells in the cat."Am.J.Vet.Res.. (In press). (2000)

Okai, Y. 等人：“猫淋巴瘤细胞多药耐药性的分子分析。”Am.J.Vet.Res..（正在出版）。

Cho, K.W.et al: "Assignment of the cat p53 tumor suppressor gene(TP53)to cat chromosome E1p14-P13 by fluorescence in situ hybridization." Cytogenet.Cell Genet.79. 145-146 (1998)

Cho, K.W.等人：“通过荧光原位杂交将猫 p53 肿瘤抑制基因 (TP53) 分配到猫染色体 E1p14-P13。”