Fundamental Research on the Cat Genome Analysis

猫基因组分析的基础研究

• 批准号: 10460138
• 负责人: TSUJIMOTO Hajime
• 金额: $ 2.62万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460138/
• 关键词: Cat; Genome analysis; FISH analysis; Oncogene; Tumor suppressor gene; Immunoglobulin gene; T-cell receptor gene; Lymphoma; 癌抑制遺伝子; 免疫グログリン遺伝子; in situ ハイブリダイゼーション; 腫瘍; p53

In the present studies on the cat genome analysis, we carried out fluorescence in situ hybridization (FISH) analysis to investigate a number of genes associated with tumorigenesis in the cat.[Oncogene] By FISH analysis, myc gene was assigned to cat chromosome F2q21.2. Fit-1 region, associated with the tumorigenesis in cat lymphomas, was shown to be present in a locus closely linked to that of myb gene on cat chromosome B2. [Tumor suppressor and cyclin dependent kinase (Cdk) in hibitor genes] FISH analysis indicated that p53 gene, p15MTS2/p16MTSI gene, p21 WAFI gene, and p27kipl gene were assigned to cat chromosome E1p14-p13, D4q12, B2, and B4, respectively. [Immunoglobulin and T-cell receptor genes] Ig heavy chain gene, Ig light (lambda) chain gene, and TCR γ chain gene were assigned to cat chromosome B3q26, D3p12-p11, and A2q12-q13, respectively. From these chromosome mapping studies, loci of a number of genes associated with the tumorigenesis in cat lymphoma and leukemia could be identified on cat genome.Moreover, from a series of chromosome analysis of cat lymphoma cells including clinical specimens, we could identify a chromosomal translocation between F2 and B3 chromosomes in cat 3201B T-lymphoma cell line. FISH analysis of this cell line revealed that myc gene on the long arm of F2 chromosome and Ig heavy chain gene on the long arm of B3 chromosome were shown to be adjacent on the on the same chromosome.The present studies were carried out by cytogenetic approaches in cats. The results of the studies will provide fundamental informations on the advanced researches on the tumorigenesis not only in cats but also in humans.

在有关CAT基因组分析的介绍研究中，我们进行了荧光原位杂交（FISH）分析，以研究CAT中与肿瘤发生相关的许多基因。[Oncogene]通过鱼类分析，将MYC基因分配给CAT CAT染色体F2Q21.2。与CAT淋巴瘤中的肿瘤发生有关的FIT-1区域被证明存在于与CAT染色体B2上的Myb基因密切相关的基因座中。 [Hibitor基因中的肿瘤抑制和细胞周期蛋白依赖性激酶（CDK）]鱼类分析表明，将p53基因，p15mts2/p16mtsi基因，p21 WAFI基因和p27Kipl基因分配给CAT CAT CAT CAT CAT CAT CAT染色体E1P14-P13，D4Q12，D4Q12，B2，B4，B4，B4，B4，B4，B4，B4，B4，B4。 [免疫球蛋白和T细胞受体基因]分别分配给CAT染色体B3Q26，D3P12-P11和A2Q12-Q13，将IG重链基因，IG光（LAMBDA）链基因和TCRγ链基因分配给CAT染色体B3Q26，D3Q26，D3Q26，D3Q26和A2Q12-Q13。从这些染色体映射研究中，可以在CAT基因组上鉴定出许多与CAT淋巴瘤和白血病中肿瘤发生相关的基因的局部性。从包括临床标本在内的猫淋巴瘤细胞的一系列染色体分析，我们可以识别出F2和B3染色体之间的染色体易位，从而确定F2和B3染色体之间的染色体分析。该细胞系的鱼类分析表明，在B3染色体长的长臂上，F2染色体长臂和Ig重链基因的MYC基因显示出在同一染色体上相邻的。当前的研究是通过猫中的细胞遗传学方法进行的。研究结果将提供有关肿瘤发生不仅在猫中而且在人类中的肿瘤发生的基本信息。

项目成果

Endo, Y.et al.: "Molecular cloning of CC-chemokine cDNAs." Vet.Immunol.Immunopathol.65. 113-123 (1998)

Endo, Y. 等人：“CC 趋化因子 cDNA 的分子克隆。”

Cho, K.W.et al.: "Assignment of the cat p53 tumor suppressor gene (TP53) to cat chromosome E1p14-p13 by fluorescence in situ hybridization."Cytogent.Cell Genet.. 79. 145-146 (1998)

Cho, K.W.等人：“通过荧光原位杂交将猫 p53 肿瘤抑制基因 (TP53) 分配给猫染色体 E1p14-p13。”Cytogent.Cell Genet.. 79. 145-146 (1998)

Cho, K.W.et al: "Assignment of the cat p53 tumor suppressor gene(TP53)to cat chromosome E1p14-P13 by fluorescence in situ hybridization." Cytogenet.Cell Genet.79. 145-146 (1998)

Cho, K.W.等人：“通过荧光原位杂交将猫 p53 肿瘤抑制基因 (TP53) 分配到猫染色体 E1p14-P13。”

Nishimura, Y.et al: "Genetic heterogeneity in the env gene of FIV clones obtained from various districts in Japan." Virus Res.57. 101-112 (1998)

Nishimura, Y.等人：“从日本各个地区获得的 FIV 克隆的 env 基因的遗传异质性。”

Okai, Y.et al.: "Molecular analysis of multidrug resistance in lymphoma cells in the cat."Am.J.Vet.Res.. (In press). (2000)

Okai, Y. 等人：“猫淋巴瘤细胞多药耐药性的分子分析。”Am.J.Vet.Res..（正在出版）。