Fundamental Research on the Cat Genome Analysis

在目前的猫基因组分析研究中，我们利用荧光原位杂交(FISH)技术研究了一些与猫肿瘤发生相关的基因。通过FISH分析，将myc基因定位于猫染色体F2q21.2。与猫淋巴瘤发生相关的FIT-1区域位于与猫染色体B2上的MYB基因紧密连锁的区域。FISH分析表明，p53基因、p15MTS2/p16MTSI基因、p21Wafi基因和p27kipl基因分别位于猫染色体E1p14-p13、D4q12、B2和B4。[免疫球蛋白和T细胞受体基因]Ig重链基因、Ig轻链基因和TcRγ链基因分别位于猫染色体B3q26、D3p12-p11和A2q12-q13。从这些染色体定位研究中，可以在猫基因组上确定与猫淋巴瘤和白血病发生相关的一些基因的位置。此外，从包括临床标本在内的一系列猫淋巴瘤细胞的染色体分析中，我们可以确定猫3201B T淋巴瘤细胞系在F2和B3染色体之间存在染色体易位。FISH分析表明，F2染色体长臂上的myc基因和B3染色体长臂上的Ig重链基因在同一染色体上相邻。这些研究结果将为猫和人类肿瘤发生的深入研究提供基础信息。

In the present studies on the cat genome analysis, we carried out fluorescence in situ hybridization (FISH) analysis to investigate a number of genes associated with tumorigenesis in the cat.[Oncogene] By FISH analysis, myc gene was assigned to cat chromosome F2q21.2. Fit-1 region, associated with the tumorigenesis in cat lymphomas, was shown to be present in a locus closely linked to that of myb gene on cat chromosome B2. [Tumor suppressor and cyclin dependent kinase (Cdk) in hibitor genes] FISH analysis indicated that p53 gene, p15MTS2/p16MTSI gene, p21 WAFI gene, and p27kipl gene were assigned to cat chromosome E1p14-p13, D4q12, B2, and B4, respectively. [Immunoglobulin and T-cell receptor genes] Ig heavy chain gene, Ig light (lambda) chain gene, and TCR γ chain gene were assigned to cat chromosome B3q26, D3p12-p11, and A2q12-q13, respectively. From these chromosome mapping studies, loci of a number of genes associated with the tumorigenesis in cat lymphoma and leukemia could be identified on cat genome.Moreover, from a series of chromosome analysis of cat lymphoma cells including clinical specimens, we could identify a chromosomal translocation between F2 and B3 chromosomes in cat 3201B T-lymphoma cell line. FISH analysis of this cell line revealed that myc gene on the long arm of F2 chromosome and Ig heavy chain gene on the long arm of B3 chromosome were shown to be adjacent on the on the same chromosome.The present studies were carried out by cytogenetic approaches in cats. The results of the studies will provide fundamental informations on the advanced researches on the tumorigenesis not only in cats but also in humans.