Fundamental Research on the Cat Genome Analysis

在目前的猫基因组分析研究中，我们进行了荧光原位杂交（FISH）分析，以研究猫中与肿瘤发生相关的一些基因。【癌基因】经FISH分析，myc基因定位于猫染色体F2q21.2。与猫淋巴瘤的肿瘤发生相关的Fit-1区域被证明存在于与猫B2染色体上的myb基因密切相关的位点上。[肿瘤抑制因子和细胞周期蛋白依赖性激酶（Tumor suppressor and cyclin dependent kinase, Cdk） in hibitgene] FISH分析显示，p53基因、p15MTS2/p16MTSI基因、p21 WAFI基因和p27kipl基因分别位于猫染色体E1p14-p13、D4q12、B2和B4上。【免疫球蛋白和t细胞受体基因】Ig重链基因、Ig轻（lambda）链基因和TCR γ链基因分别被分配到猫染色体B3q26、D3p12-p11和A2q12-q13上。从这些染色体定位研究中，可以在猫基因组中鉴定出与猫淋巴瘤和白血病肿瘤发生相关的一些基因位点。此外，通过对猫淋巴瘤细胞包括临床标本的一系列染色体分析，我们发现猫3201B t淋巴瘤细胞系中F2染色体和B3染色体之间存在染色体易位。对该细胞系的FISH分析显示，F2染色体长臂上的myc基因和B3染色体长臂上的Ig重链基因在同一染色体上相邻。本研究采用细胞遗传学方法在猫身上进行。研究结果将为进一步研究猫及人类肿瘤发生机制提供基础信息。

In the present studies on the cat genome analysis, we carried out fluorescence in situ hybridization (FISH) analysis to investigate a number of genes associated with tumorigenesis in the cat.[Oncogene] By FISH analysis, myc gene was assigned to cat chromosome F2q21.2. Fit-1 region, associated with the tumorigenesis in cat lymphomas, was shown to be present in a locus closely linked to that of myb gene on cat chromosome B2. [Tumor suppressor and cyclin dependent kinase (Cdk) in hibitor genes] FISH analysis indicated that p53 gene, p15MTS2/p16MTSI gene, p21 WAFI gene, and p27kipl gene were assigned to cat chromosome E1p14-p13, D4q12, B2, and B4, respectively. [Immunoglobulin and T-cell receptor genes] Ig heavy chain gene, Ig light (lambda) chain gene, and TCR γ chain gene were assigned to cat chromosome B3q26, D3p12-p11, and A2q12-q13, respectively. From these chromosome mapping studies, loci of a number of genes associated with the tumorigenesis in cat lymphoma and leukemia could be identified on cat genome.Moreover, from a series of chromosome analysis of cat lymphoma cells including clinical specimens, we could identify a chromosomal translocation between F2 and B3 chromosomes in cat 3201B T-lymphoma cell line. FISH analysis of this cell line revealed that myc gene on the long arm of F2 chromosome and Ig heavy chain gene on the long arm of B3 chromosome were shown to be adjacent on the on the same chromosome.The present studies were carried out by cytogenetic approaches in cats. The results of the studies will provide fundamental informations on the advanced researches on the tumorigenesis not only in cats but also in humans.