In vivo imaging of clock gene expression in conscious animals
In vivo imaging of clock gene expression in conscious animals
批准号:
19390057
负责人:
HONMA Sato
金额:
$12.23万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009
中文摘要
点击翻译按钮获取中文摘要
英文摘要
本研究は、in vivoで、無麻酔・無拘束動物から時計遺伝子発現リズムを計測し、視交叉上核(SCN)に局在する哺乳類の中枢時計の行動リズム制御メカニズムを明らかにすることを目的として行った。時計遺伝子Per1プロモータ支配下に発光酵素ルシフェラーゼを発現するPer1-lucマウスを用い、自発行動量と光ファイバーによるSCNの時計遺伝子発現を同時計系を構築し、1分毎の連続測定を行った。その結果、SCN、嗅球いずれからも、安定したPer1-lucのフリーランリズムが計測された。さらに、光同調や位相反応を示すことから、内因性振動を反映していることが確認できた。
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Clock mechanisms for seasonal adaptation : Morning and evening oscillators in the suprachiasmatic nucleus
季节适应的时钟机制:视交叉上核的早晨和晚上振荡器
DOI:
--
发表时间:
2008
期刊:
Sleep Biol. Rhythms 6
影响因子:
--
作者:
[Honma, S]
通讯作者:
S
Bioluminescence imaging for assessing heterogeneous cell functions in the central circadian clock
用于评估中央生物钟中异质细胞功能的生物发光成像
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Nishiguchi KM, Yasuma TR, Tomida D, Nakamura M, Ishikawa K, Kikuchi M, Ohmi Y, Niwa T, Hamajima N, Furukawa K, Terasaki H, Horinouchi T, Honma S]
通讯作者:
Honma S
DOI:
10.1210/en.2007-0010
发表时间:
2007-07-01
期刊:
ENDOCRINOLOGY
影响因子:
4.8
作者:
[Hayasaka, Naomi, Yaita, Tsuyoshi, Shibata, Shigenobu]
通讯作者:
Shibata, Shigenobu
DOI:
10.1073/pnas.0607713104
发表时间:
2007-05-01
期刊:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子:
11.1
作者:
[Inagaki, Natsuko, Honma, Sato, Honma, Ken-ichi]
通讯作者:
Honma, Ken-ichi
光でみる生体リズム:細胞時計のダイナミズム
用光看到的生物节律:细胞时钟的动态
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[谷内一彦, 田代学, 本間さと]
通讯作者:
本間さと
共 45 条
Hierarchical self-organization of circadian system: synchronization of cellular oscillators and pacemaker coupling
-
批准号:15H04679
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.15万
-
财政年份:2015
-
负责人:HONMA Sato
-
依托单位:
Measurement of transcription activity in freely moving mice: Ultradian rhythms and their physiological roles
-
批准号:15K12763
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.33万
-
财政年份:2015
-
负责人:HONMA Sato
-
依托单位:
Visualizing gene expression in response to environmental stimuli :real-time bioluminescence imaging in freely moving mice
-
批准号:22659047
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.0万
-
财政年份:2010
-
负责人:HONMA Sato
-
依托单位:
Integration of multi-oscillator circadian clock: in vivo and ex vivo imaging of clock gene expression
-
批准号:21390064
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$12.06万
-
财政年份:2009
-
负责人:HONMA Sato
-
依托单位:
Central and peripheral clocks; Mechanisms of temporal integration
-
批准号:17390059
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.41万
-
财政年份:2005
-
负责人:HONMA Sato
-
依托单位:
Circadian rhythm generation mechanism : multi-reporter analysis of interlocked molecular feedback loops
-
批准号:15390068
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.86万
-
财政年份:2003
-
负责人:HONMA Sato
-
依托单位:
Circadian Rhythm Generation and Tissue Specific Functions of the Clock genes
-
批准号:13670054
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.62万
-
财政年份:2001
-
负责人:HONMA Sato
-
依托单位:
Multichannel analysis and monitoring of multiple gene expressions by using bioluminescent reporters
-
批准号:12557005
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$8.0万
-
财政年份:2000
-
负责人:HONMA Sato
-
依托单位:
Function of Clock Genes : Examination in Cultured Suprachiasmatic Nucleus
-
批准号:11670057
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.5万
-
财政年份:1999
-
负责人:HONMA Sato
-
依托单位:
Coupling of suprachiasmatic neuronal activity and peptide rhythms : pacemaker structures
-
批准号:09670065
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.05万
-
财政年份:1997
-
负责人:HONMA Sato
-
依托单位:
Development of long-term monitoring system of intracellular Ca^<2+> levels by Aequorin gene transfection
-
批准号:08557006
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$8.96万
-
财政年份:1996
-
负责人:HONMA Sato
-
依托单位:
Photic entrainment: Mechanisms in the SCN and rhythm expression to the pineal.
-
批准号:03670071
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.28万
-
财政年份:1991
-
负责人:HONMA Sato
-
依托单位:
Ontogeny of the circadian oscillation: zeitgebers of maternal netrainment and its mechanism.
-
批准号:61570081
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.15万
-
财政年份:1986
-
负责人:HONMA Sato
-
依托单位:
海外基金