Elucidation of acute actin polymerization signaling using single molecule imaging of filament end factors
Elucidation of acute actin polymerization signaling using single molecule imaging of filament end factors
批准号:
19390075
负责人:
WATANABE Naoki
金额:
$12.23万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2008
中文摘要
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英文摘要
多くの生命機能は細胞の形態変化と密接に連関し、動的なアクチン重合が多く関わっている。まず、アクチン脱重合因子AIP1 の単分子可視化により、細胞伸展部で毎秒280アクチン分子長の線維に1回線維が切断されることを見出した. これは主要なアクチン重合端形成機構と考えられたArp2/3複合体による重合核形成の15倍の高頻度である. また, フォルミン蛋白質mDia1がアクチン単量体増加にトリガーされ, プロセッシブに線維伸長させるアクチン線維修復機構を見出した. 線維崩壊から迅速にアクチン再重合させるフィードバック機構が明らかとなった。
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Rapid actin polymer restoration mechanism by mDia1 and othe actin nucleators regulated by concentration fluctuation of G-actin
mDia1 和其他肌动蛋白成核剂受 G-肌动蛋白浓度波动调节的快速肌动蛋白聚合物修复机制
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Higashida C., Suetsugu S., Narumiya S., Watanabe N.]
通讯作者:
Watanabe N.
Shootinl interacts with actin retrograde flow and L1-CAM to promote axon outgrowth
Shootinl 与肌动蛋白逆行流和 L1-CAM 相互作用促进轴突生长
DOI:
--
发表时间:
2008
期刊:
J. Cell. Biol. 181
影响因子:
--
作者:
[T Matsumoto, et. al., 松本健志, 松本健志, T. Shimada]
通讯作者:
T. Shimada
Molecular kinetics of AIP1, a cofilin-dependent actin barbed end interacting protein. in lamellipodia: Evidence for frequent filament severing.
AIP1 的分子动力学,AIP1 是一种肌动蛋白丝切蛋白依赖性肌动蛋白末端倒刺相互作用蛋白。
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Tomatsu, H., Takano, J., Takahashi, H., Watanabe-Takahashi A., Shibagaki, N., Fujiwara, T., Nishizawa NK, Naoki Watanabe]
通讯作者:
Naoki Watanabe
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Watanabe. N., Higashida, C.]
通讯作者:
C.
A paradoxical effect of actin monomer inhibitors to increase drug-free G-actin in cells : an unexpected role of G-actiti revealed by pharmacokinetic simulation.
肌动蛋白单体抑制剂增加细胞中无药 G-肌动蛋白的矛盾效应:药代动力学模拟揭示了 G-肌动蛋白的意外作用。
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Watanabe, N., Higashida, C.]
通讯作者:
C.
共 24 条
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A Theoretical and Empirical Research on Dynamic Industry Structure : Research and Development, License, Endogenous Coalition Formation
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Single-molecule imaging analysis of cell structural reorganization processes downstream of G protein signaling
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Surface reactions of atoms and the mechanism of deuterium fractionation on inter Stellar dusts at the very low temperature
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Development of new therapy for pancreas cancer using PPAR γ-ligand targeting hTERT.
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Establishment of gene therapy for panceratic cancer, targeting genes for the components of telomerase and telomeric-repeat binding proteins.
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Helicobacter pylori infection augments telomerase activity in gastric cancer and noncancerous tissues
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