Single-molecule imaging analysis of cell structural reorganization processes downstream of G protein signaling
Single-molecule imaging analysis of cell structural reorganization processes downstream of G protein signaling
批准号:
17079004
负责人:
WATANABE Naoki
金额:
$34.88万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2009
中文摘要
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英文摘要
Using fluorescence single-molecule imaging which directly visualizes remodeling processes of the actin cytoskeleton, this research project has revealed a novel actin polymer regeneration mechanism by which an increase in the cellular monomeric actin concentration provokes rapid actin nucleation by Formin homology proteins. This project has also quantitatively proved the frequent actin filament disruption in the cell. In addition, through various types of cell imaging analysis, the roles of Rho family GTPases in cell migration and inhibitor-induced conformational regulation of a protein kinase, Abl, have been elucidated.
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Rapid actin polymer restoration against mechanical force : Roles of G-and F-actin homeostasis and mDial
对抗机械力的快速肌动蛋白聚合物恢复:G-和 F-肌动蛋白稳态和 mDial 的作用
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Watanabe. N., Higashida, C., Mizuno, H.]
通讯作者:
H.
(speaker) Rapid actin polymer restoration mechanism by mDia1 and other actin nucleators regulated by concentration fluctuation of G-actin.
(演讲者)mDia1 和其他肌动蛋白成核剂受 G-肌动蛋白浓度波动调节的快速肌动蛋白聚合物修复机制。
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Higashida C., Suetsugu S., Narumiya S., Watanabe N.]
通讯作者:
Watanabe N.
非侵襲・可視化技術ハンドブック-ナノ・バイオ・医療から情報システムまで-(共著)第8章第10節 アクチン細胞骨格のダイナミクスの可視化
非侵入/可视化技术手册-从纳米/生物/医学到信息系统-(合着)第8章第10节肌动蛋白细胞骨架动力学的可视化
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[渡邊 直樹(小川誠二, 上野照剛編)]
通讯作者:
上野照剛編)
アクチン細胞骨格とアクチン調節タンパク質実験医学第24巻第13号増刊形と運動を司る細胞のダイナミクス
肌动蛋白细胞骨架和肌动蛋白调节蛋白实验医学第24卷第13期特别版控制运动的细胞的形状和动力学
DOI:
--
发表时间:
2006
期刊:
影响因子:
--
作者:
[馬渕一誠, 渡邊直樹, 寺崎朝子, 高稲正勝, 斧正一郎]
通讯作者:
斧正一郎
アクチンダイナミクス蛋白質核酸酵素
肌动蛋白动力学蛋白质核酸酶
DOI:
--
发表时间:
2006
期刊:
影响因子:
--
作者:
[馬渕一誠, 渡邊直樹, 寺崎朝子, 高稲正勝, 斧正一郎, 西澤直子, Yusuke Nagai, 渡邊直樹]
通讯作者:
渡邊直樹
共 62 条
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Residency Matching Mechanisms and Changes in Geographic Distribution of Doctors in Japan
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nalyses of Disciplinary Mechanism towards Executives: Lesson from
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Payoff Allocation Problem in Patent Pools: Coalition Formation, Investment in Development, and Efficiency
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Deuterium fractionation of water molecule via grain surface reactions at very low temperatures
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Ice surface processes: tunneling reactions and isotope fractionation
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Development of auto-IAP-antibody screening array for diagnosing cancer patients.
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Elucidation of acute actin polymerization signaling using single molecule imaging of filament end factors
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Role of cosmic-grain surface reactions in the deuterium fractionationof interstellar molecules
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A Theoretical and Empirical Research on Dynamic Industry Structure : Research and Development, License, Endogenous Coalition Formation
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Development of auto-IAP-antibody screening array for diagnosing cancer patients
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依托单位:
Actin-polymerization-driven molecular dynamics of Formin homology proteins in live cells
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Surface reactions of atoms and the mechanism of deuterium fractionation on inter Stellar dusts at the very low temperature
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Development of new therapy for pancreas cancer using PPAR γ-ligand targeting hTERT.
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Identification of intracellular signaling cascade of Rho and mDia1 based on high sensitivity live-cell imaging
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Establishment of gene therapy for panceratic cancer, targeting genes for the components of telomerase and telomeric-repeat binding proteins.
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The social and cultural Research on German Enlightenment
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Helicobacter pylori infection augments telomerase activity in gastric cancer and noncancerous tissues
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Specific interaction of pancreatic elastase and leukocytes to produce oxygen radicals and its implication in pancreatitis
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依托单位:
海外基金