Subcellular co-localisation of interacting proteins that control maturation in mammalian eggs

哺乳动物卵子(卵母细胞)在受精前的最终成熟包括一系列蛋白激酶和磷酸酶之间的相互作用，这些蛋白在细胞质和细胞核之间穿梭，以及微管的组装和拆卸。在这个项目中，我们提出了参与卵母细胞成熟的相互作用蛋白以严格的时间和空间方式共同定位于卵母细胞的亚细胞位置。蛋白质在卵母细胞中的共定位被认为是确保有时间限制的细胞事件的协调以及卵母细胞为受精和早期胚胎发育做好适当准备的基本机制。这对通过核移植进行体外受精和克隆具有重要的意义。

The final maturation of mammalian eggs (oocytes) before fertilisation involves a cascade of interactions between protein kinases and phosphatases, the shuttling of these proteins between cytoplasm and nucleus, and microtubule assembly and disassembly. In this project we have proposed that interacting proteins involved in oocyte maturation are co-localised at subcellular sites in oocytes, in a strict temporal and spatial manner. The co-localisation of proteins in oocytes is considered a fundamental mechanism that ensures coordination of time-bound cellular events and proper preparation of oocytes for fertilisation and early embryo development. There are important implications for IVF and cloning by nuclear transfer.