DNA methylation in IVF and cloned embryos

体外受精（IVF）和克隆是新的辅助生殖技术，随着时间的推移，将彻底改变澳大利亚重要经济动物的遗传改良。 IVF和克隆可能与非典型基因表达导致的异常生长有关。 DNA甲基化是基因调控的核心，决定了哪些基因被表达。 在这个项目中，一种新的技术将用于量化自然受孕，IVF和克隆胚胎中的DNA甲基化。 这些信息将用于改进体外受精和克隆技术，以便这些技术能够有信心地应用于提高澳大利亚畜牧业的国际竞争力。

In vitro fertilisation (IVF) and cloning are new assisted reproductive technologies that in time will revolutionise genetic improvement of Australia's economically important animals. IVF and cloning can be associated with aberrant growth that results from atypical gene expression. DNA methylation is central to gene regulation and determines which genes are expressed. In this project a new technique will be used to quantify DNA methylation in naturally conceived, IVF and cloned embryos. The information will be used to improve IVF and cloning so that these technologies can be applied with confidence to increase the international competitiveness of Australia's livestock industries.