Production and identification of favorable characteristics of Brassica napus-Raphanus sativus moosomic adittion lines

甘蓝型油菜-萝卜杂交系的产生及优良性状鉴定

• 批准号: 19580002
• 负责人: KANEKO Yukio
• 金额: $ 2.08万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580002/
• 关键词: ダイコン; ナタネ; 染色体添加系統; 雄性不稔; 花粉稔性回復因子; 花色; 核置換系統; 核復帰系統; 異種染色体添加系統; 根こぶ病抵抗性; 種属間交雑の不稔性; 花粉経由による染色体伝達; 種属問交雑の不稔性

In this study, we tried to breed alloplasmic and autoplasmic Brassica napus-Raphanus sativus monosomic addition lines (MALs) mediated by the synthetic amphidiploid Raphanobrassica (RA89 and Rb63 lines), and also to survey favorable characteristics for B.napus breeding using them.In 2007, alloplasmic MAL with 8 types (a～i) except for the h-type and autoplasmic MAL with complete 9 types were produced using 175 RAPD markers specific to R-genome. In characteristics of the MALs, most of alloplasmic MAL having the radish cytoplasm showed male sterility and only the a-type produced normal flower and had a high pollen fertility (88%), suggesting to have fertility restoring gene(s) for male sterility in alloplasmic line.In 2008, we studied (1) identification and evaluation of clubroot resistance (CR) of radish chromosome, and (2) embryogenesis of MAL with d-chromosome of radish inducing low seed fertility. (1) Among the 9 types of MALs, only the c-type showed strong resistance (IC=73%, ID=0.7), … More on the other hand, other types showed complete susceptibility (IC=100%, ID=3.0). The result suggested that the major CR gene(s) may be located on the c-chromosome of the radish genome. (2) In 3 weeks after backcrossing of MALs × B.napus, embryos in all types except for the d-type were grown from torpedo (T) to mature (M) stage, on the other hand, one in d-type were globular (G) to heart (H) stage following to chlorosis. It was assumed that the low seed fertility in the d-type was induced by embryo underdevelopment.In 2009, for development of disomic addition lines (DALs), we studied ; (1) improvement of male transmission rate of radish chromosome in MALs by limited pollination, and (2) crossing with the same addition lines by both normal and limited pollination methods. (1) the 7 types except for the b- and c-types were advanced male transmission rate of alien chromosome by limited pollination (7.0～70.3%) in comparison with normal one (2.9～54.3%). (2) a large number of seed were successfully obtained with a range of 13.2 seeds per pod for the i-type (totally 787 seeds) to 1.7 for the g-type (132 seeds). From results of (1) and (2), it may be developed DALs in the near future. Less

本研究以人工合成的双二倍体萝卜为材料，通过诱导异源和同源细胞质的甘蓝型油菜-萝卜单体异附加系，（RA 89和Rb 63系），并调查利用它们进行B.napus育种的有利特性。利用175个R基因组特异性RAPD标记，获得了除h型外的8种（a~i）异质MAL和9种完整的同源MAL。2008年，我们开展了以下研究：（1）萝卜染色体抗根肿病鉴定与评价，（2）萝卜染色体抗根肿病鉴定与评价，（3）萝卜染色体抗根肿病鉴定与评价，（4）萝卜染色体抗根肿病鉴定与评价，（5）萝卜染色体抗根肿病鉴定与评价，（6）萝卜染色体抗根肿病鉴定与评价，（7）萝卜染色体抗根肿病鉴定与评价，（8）萝卜染色体抗根肿病鉴定与评价，（9）萝卜染色体抗根肿病鉴定与评价，（10）萝卜染色体抗根肿（2）萝卜d染色体的MAL胚胎发生诱导低育性种子。(1)在9种MALs中，只有C型表现出较强的抗性（IC= 73%，ID=0.7）， ...更多信息 而其他类型则完全敏感（IC= 100%，ID=3.0）。结果表明，萝卜的主效CR基因可能位于萝卜基因组的C染色体上。(2)回交3周后，除d型胚外，其余类型胚均从鱼雷期（T）发育到成熟期（M），d型胚中有1个胚从球形期（G）发育到心形期（H），并随失绿而退化。2009年，为了选育二体异附加系，本研究采用限制授粉的方法提高萝卜二体异附加系的雄性传递率，并采用常规授粉和限制授粉的方法与相同的二体异附加系进行杂交。(1)除B-和C-型外，其余7种类型的有限授粉雄传率（7.0~70.3%）均高于正常授粉雄传率（2.9~54.3%）。(2)成功地获得了大量的种子，I型的每荚13.2粒种子（总共787粒种子）到G型的每荚1.7粒种子（132粒种子）。根据（1）和（2）的结果，在不久的将来可能会开发DAL。少

项目成果

Evaluation of clubroot resistance of radish chroino some using a Brassica napus-Raphanus sativus monos omic addition line

利用甘蓝型油菜-萝卜单组学附加系评价萝卜根肿病抗性

• 发表时间: 2008
• 作者: 筒井;ら;筒井康太・赤羽美智子・房相佑・金子幸雄・松澤康男;赤羽 ら;Akaba et al.
• 通讯作者: Akaba et al.

Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

• DOI: 10.1270/jsbbs.59.109
• 发表时间: 2009-06-01
• 期刊: BREEDING SCIENCE
• 影响因子: 2.4
• 作者: Akaba, Michiko;Kaneko, Yukio;Matsuzawa, Yasuo
• 通讯作者: Matsuzawa, Yasuo

ダイコン1染色体添加型ナタネの少量受粉による添加染色体の雄性伝達率の向上

萝卜加一条染色体油菜小授粉提高附加染色体雄性传递率

• 发表时间: 2009
• 作者: 筒井;ら
• 通讯作者: ら

Raphanobrassica 2 系統とナタネとの交雑およびその後代植物の育成

萝卜 2 系与油菜杂交及其后代植物的育种

• 作者: 筒井;ら;筒井康太・赤羽美智子・房相佑・金子幸雄・松澤康男;赤羽 ら;Akaba et al.;赤羽ら;赤羽美智子・金子幸雄・畠山勝徳・石田正彦・房相佑・松澤康男;赤羽美智子・房相佑・金子幸雄;赤羽美智子;赤羽美智子・金子幸雄・中田好美・房相佑・松澤康男
• 通讯作者: 赤羽美智子・金子幸雄・中田好美・房相佑・松澤康男

11. Distant Hybridization In Biology and Breeding of Crucifers (Ed. SKGupta)

11. 十字花科植物生物学和育种中的远距离杂交（Ed. SKGupta）

• 作者: Kaneko,Y.;S. W. Bang;Y. Matsuzawa;Kaneko et al.
• 通讯作者: Kaneko et al.