Production and identification of favorable characteristics of Brassica napus-Raphanus sativus moosomic adittion lines

甘蓝型油菜-萝卜杂交系的产生及优良性状鉴定

基本信息

批准号：
19580002
负责人：
KANEKO Yukio
金额：
$ 2.08万
依托单位：
Utsunomiya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

In this study, we tried to breed alloplasmic and autoplasmic Brassica napus-Raphanus sativus monosomic addition lines (MALs) mediated by the synthetic amphidiploid Raphanobrassica (RA89 and Rb63 lines), and also to survey favorable characteristics for B.napus breeding using them.In 2007, alloplasmic MAL with 8 types (a～i) except for the h-type and autoplasmic MAL with complete 9 types were produced using 175 RAPD markers specific to R-genome. In characteristics of the MALs, most of alloplasmic MAL having the radish cytoplasm showed male sterility and only the a-type produced normal flower and had a high pollen fertility (88%), suggesting to have fertility restoring gene(s) for male sterility in alloplasmic line.In 2008, we studied (1) identification and evaluation of clubroot resistance (CR) of radish chromosome, and (2) embryogenesis of MAL with d-chromosome of radish inducing low seed fertility. (1) Among the 9 types of MALs, only the c-type showed strong resistance (IC=73%, ID=0.7), … More on the other hand, other types showed complete susceptibility (IC=100%, ID=3.0). The result suggested that the major CR gene(s) may be located on the c-chromosome of the radish genome. (2) In 3 weeks after backcrossing of MALs × B.napus, embryos in all types except for the d-type were grown from torpedo (T) to mature (M) stage, on the other hand, one in d-type were globular (G) to heart (H) stage following to chlorosis. It was assumed that the low seed fertility in the d-type was induced by embryo underdevelopment.In 2009, for development of disomic addition lines (DALs), we studied ; (1) improvement of male transmission rate of radish chromosome in MALs by limited pollination, and (2) crossing with the same addition lines by both normal and limited pollination methods. (1) the 7 types except for the b- and c-types were advanced male transmission rate of alien chromosome by limited pollination (7.0～70.3%) in comparison with normal one (2.9～54.3%). (2) a large number of seed were successfully obtained with a range of 13.2 seeds per pod for the i-type (totally 787 seeds) to 1.7 for the g-type (132 seeds). From results of (1) and (2), it may be developed DALs in the near future. Less

在这项研究中，我们试图繁殖异质和自质甘蓝纳普斯拉法氏抗酸性添加线（MALS）（MALS）（MALS）介导的合成两极型raphanobrassica（RA89和RB63系）介导使用特定于R-Genome的175个RAPD标记产生了具有9种类型的自质MAL。 In characteristics of the MALs, most of alloplasmic MAL having the radish cytoplasm shown male sterility and only the a-type produced normal flower and had a high pollen fertility (88%), suggesting to have fertility restoring gene(s) for male sterility in alloplasmic line.In 2008, we studied (1) identification and evaluation of clubbroot resistance (CR) of radish chromosome, and (2)萝卜的D-染色体诱导低种子生育能力的Mal的胚胎发生。（1）在9种类型的MALS中，只有C型显示出强电阻（IC = 73％，ID = 0.7），…另一方面，其他类型显示的其他类型完全敏感（IC = 100％，ID = 3.0）。结果表明，主要的Cr基因可能位于萝卜基因组的C染色体上。（2）在Mals×B.Napus反向交叉后的3周内，除D型外，所有类型的胚胎从鱼雷（T）生长到成熟（M）阶段，另一方面，D型中的一个是全球（G）的全球（G）到心脏（H）到心脏（H）阶段（H）阶段。假定D型中的低种子生育能力是由胚胎欠发达诱导的。（1）通过有限的授粉来提高MAL的男性萝卜染色体的传播速率，以及（2）通过正常和有限的授粉方法与相同的添加线交叉。（1）与正常一（2.9-54.3％）相比，有限的授粉（7.0-70.3％）以有限的授粉（7.0-70.3％）（2.9-54.3％）的7种类型是外星染色体的晚期男性传播率。（2）对于G型（132种种子），I型（完全787种子）的每荚（完全787种子）成功获得了大量种子，每荚的13.2种子（完全787种子）。根据（1）和（2）的结果，它可以在不久的将来开发。较少的