Production and identification of favorable characteristics of Brassica napus-Raphanus sativus moosomic adittion lines

甘蓝型油菜-萝卜杂交系的产生及优良性状鉴定

• 批准号: 19580002
• 负责人: KANEKO Yukio
• 金额: $ 2.08万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580002/
• 关键词: ダイコン; ナタネ; 染色体添加系統; 雄性不稔; 花粉稔性回復因子; 花色; 核置換系統; 核復帰系統; 異種染色体添加系統; 根こぶ病抵抗性; 種属間交雑の不稔性; 花粉経由による染色体伝達; 種属問交雑の不稔性

In this study, we tried to breed alloplasmic and autoplasmic Brassica napus-Raphanus sativus monosomic addition lines (MALs) mediated by the synthetic amphidiploid Raphanobrassica (RA89 and Rb63 lines), and also to survey favorable characteristics for B.napus breeding using them.In 2007, alloplasmic MAL with 8 types (a～i) except for the h-type and autoplasmic MAL with complete 9 types were produced using 175 RAPD markers specific to R-genome. In characteristics of the MALs, most of alloplasmic MAL having the radish cytoplasm showed male sterility and only the a-type produced normal flower and had a high pollen fertility (88%), suggesting to have fertility restoring gene(s) for male sterility in alloplasmic line.In 2008, we studied (1) identification and evaluation of clubroot resistance (CR) of radish chromosome, and (2) embryogenesis of MAL with d-chromosome of radish inducing low seed fertility. (1) Among the 9 types of MALs, only the c-type showed strong resistance (IC=73%, ID=0.7), … More on the other hand, other types showed complete susceptibility (IC=100%, ID=3.0). The result suggested that the major CR gene(s) may be located on the c-chromosome of the radish genome. (2) In 3 weeks after backcrossing of MALs × B.napus, embryos in all types except for the d-type were grown from torpedo (T) to mature (M) stage, on the other hand, one in d-type were globular (G) to heart (H) stage following to chlorosis. It was assumed that the low seed fertility in the d-type was induced by embryo underdevelopment.In 2009, for development of disomic addition lines (DALs), we studied ; (1) improvement of male transmission rate of radish chromosome in MALs by limited pollination, and (2) crossing with the same addition lines by both normal and limited pollination methods. (1) the 7 types except for the b- and c-types were advanced male transmission rate of alien chromosome by limited pollination (7.0～70.3%) in comparison with normal one (2.9～54.3%). (2) a large number of seed were successfully obtained with a range of 13.2 seeds per pod for the i-type (totally 787 seeds) to 1.7 for the g-type (132 seeds). From results of (1) and (2), it may be developed DALs in the near future. Less

本研究以人工合成的双二倍体萝卜(RA89和Rb63)为材料，培育了甘蓝型油菜-萝卜单体附加系(MAL)，并探讨了它们在甘蓝型油菜育种中的有利特性。2007年，利用175个R-基因组特异的RAPD标记，共获得了除h型外的8个类型(a~i)的异质油菜单体附加系和9个完整类型的自质MAL。在雄性不育系的特征上，大部分具有萝卜细胞质的异质雄性不育系表现为雄性不育，只有a型雄性不育系开花正常，花粉育性较高(88%)，这表明异质系具有雄性不育恢复基因(S)。(1)在9种MAL中，只有C型表现出较强的耐药性(IC=73%，ID=0.7)，…另一方面，其他类型完全敏感(IC=100%，ID=3.0)。这一结果表明，主CR基因(S)可能位于萝卜基因组的c-染色体上。(2)MALS×甘蓝型油菜回交后3周，除d型外，其余类型的胚胎均从鱼雷(T)发育到成熟(M)期，而d型中的1个在黄化后发育为球形(G)到心形(H)期。2009年，为了培育二体附加系，我们进行了以下研究：(1)通过限量授粉提高萝卜染色体雄性传递率；(2)通过正常授粉和限量授粉两种方法与相同附加系杂交。(1)除b型和c型外，其余7种类型的有限授粉异源染色体的雄性传递率(7.0~70.3%)均高于正常授粉的雄性传递率(2.9~54.3%)。(2)获得了大量种子，I型(共787粒)每荚种子数为13.2粒，G型(132粒)为1.7粒。根据(1)和(2)的结果，它可能在不久的将来发展为DALS。较少

项目成果

Evaluation of clubroot resistance of radish chroino some using a Brassica napus-Raphanus sativus monos omic addition line

利用甘蓝型油菜-萝卜单组学附加系评价萝卜根肿病抗性

• 发表时间: 2008
• 作者: 筒井;ら;筒井康太・赤羽美智子・房相佑・金子幸雄・松澤康男;赤羽 ら;Akaba et al.
• 通讯作者: Akaba et al.

Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

• DOI: 10.1270/jsbbs.59.109
• 发表时间: 2009-06-01
• 期刊: BREEDING SCIENCE
• 影响因子: 2.4
• 作者: Akaba, Michiko;Kaneko, Yukio;Matsuzawa, Yasuo
• 通讯作者: Matsuzawa, Yasuo

ダイコン1染色体添加型ナタネの少量受粉による添加染色体の雄性伝達率の向上

萝卜加一条染色体油菜小授粉提高附加染色体雄性传递率

• 发表时间: 2009
• 作者: 筒井;ら
• 通讯作者: ら

Raphanobrassica 2 系統とナタネとの交雑およびその後代植物の育成

萝卜 2 系与油菜杂交及其后代植物的育种

• 作者: 筒井;ら;筒井康太・赤羽美智子・房相佑・金子幸雄・松澤康男;赤羽 ら;Akaba et al.;赤羽ら;赤羽美智子・金子幸雄・畠山勝徳・石田正彦・房相佑・松澤康男;赤羽美智子・房相佑・金子幸雄;赤羽美智子;赤羽美智子・金子幸雄・中田好美・房相佑・松澤康男
• 通讯作者: 赤羽美智子・金子幸雄・中田好美・房相佑・松澤康男

11. Distant Hybridization In Biology and Breeding of Crucifers (Ed. SKGupta)

11. 十字花科植物生物学和育种中的远距离杂交（Ed. SKGupta）

• 作者: Kaneko,Y.;S. W. Bang;Y. Matsuzawa;Kaneko et al.
• 通讯作者: Kaneko et al.