Study on interaction among plant growth regulators for morphology in Phalaenopsis

从细胞分裂素（CK）和赤霉素（GA）相互作用的角度研究了蝴蝶兰低温开花的机理。低温处理14 d后，腋芽CK浓度升高，腋芽发育的起始时间与低温处理14 d相近。芽中GA浓度峰值出现在低浓度处理后28 d，芽中GA浓度峰值出现在低浓度处理后26 d。施用外源CK和GA诱导腋芽在高温不开花条件下萌发。此外，在不同温度条件下，施用GA均能诱导植株顶端分生组织开花。因此，我们认为蝴蝶兰的开花是由GA诱导的。在国际上首次发现蝴蝶兰的顶端分生组织诱导成花。由于这种现象可能有助于生产具有园艺价值的新型植物，因此申请了专利。

Mechanisms of flowering by low temperature of Phalaenopsis were investigated in terms of interaction between cytokinin (CK) and gibberellin (GA). CK concentration in axillary bud rose at 14 days after low temperature treatment (Low), and initiation of axillary bud development followed close on this peak. Peak of GA concentration in the bud and spiking from the bud appeared at 28 days and at 26 days after Low, respectively. Application of exogenous CK and GA induced spiking from axillary bud under non-flowering condition, high temperature. In addition, GA application induced flowering at apical meristem regardless of temperature conditions. Thus, it was suggested that flowering of Phalaenopsis was induced by GA. The floral induction of apical meristem of Phalaenopsis was found for the first time in the world. Because this phenomenon might contribute for production of novel plants which is valuable plant for horticulture, a patent was submitted.