Study on interaction among plant growth regulators for morphology in Phalaenopsis

通过细胞分裂素（CK）与赤霉素（GA）的互作，研究了低温诱导蝴蝶兰开花的机理。低温处理后14 d，腋芽中CK浓度开始上升，腋芽发育开始于低温处理后14 d。芽中GA浓度的高峰和芽中GA浓度的高峰分别出现在Low后28天和26天。在非开花、高温条件下，应用外源CK和GA诱导腋芽成穗。此外，GA应用诱导开花在顶端分生组织，无论温度条件。因此，初步认为GA诱导了蝴蝶兰的开花。蝴蝶兰顶端分生组织的成花诱导在国际上尚属首次。由于这种现象可能有助于生产新的植物，这是有价值的植物园艺，提交了专利。

Mechanisms of flowering by low temperature of Phalaenopsis were investigated in terms of interaction between cytokinin (CK) and gibberellin (GA). CK concentration in axillary bud rose at 14 days after low temperature treatment (Low), and initiation of axillary bud development followed close on this peak. Peak of GA concentration in the bud and spiking from the bud appeared at 28 days and at 26 days after Low, respectively. Application of exogenous CK and GA induced spiking from axillary bud under non-flowering condition, high temperature. In addition, GA application induced flowering at apical meristem regardless of temperature conditions. Thus, it was suggested that flowering of Phalaenopsis was induced by GA. The floral induction of apical meristem of Phalaenopsis was found for the first time in the world. Because this phenomenon might contribute for production of novel plants which is valuable plant for horticulture, a patent was submitted.