Characterization of a novel enzyme involved in the degradation of plant proteoglycans

参与植物蛋白多糖降解的新型酶的表征

• 批准号: 19580112
• 负责人: KANEKO Satoshi
• 金额: $ 2.91万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580112/
• 关键词: 酵素利用学; 糖質関連酵素; アラビノガラクタン-プロテイン; 糖加水分解酵素; Streptomyces avermitilis; アラビノピラノシダーゼ; アラビノガラクタンープロテイン

A novel enzyme, beta-L-arabinopyranosidase, that liberates arabinose from thickening agent arabinogalactan was isolated from Streptomyces avermitilis, and the three dimentional structure was resolved. The enzyme belongs to the glycoside hydrolase family as same as alpha-D-galactosidases. One aspartic acid in the catalytic pocket of alpha-D-galactosidase changed to glutamic acid in that of beta-L-arabinopyranosidase, and the residue modulates the enzyme activity. Addifionally, it has an arabinopyranose-binding module.

从阿维链霉菌中分离出一种新型酶，β-L-阿拉伯吡喃糖苷酶，可从增稠剂阿拉伯半乳聚糖中释放出阿拉伯糖，并解析了其三维结构。该酶与α-D-半乳糖苷酶一样属于糖苷水解酶家族。 α-D-半乳糖苷酶催化口袋中的一个天冬氨酸转变为β-L-阿拉伯吡喃糖苷酶催化口袋中的谷氨酸，并且该残基调节酶活性。此外，它还具有阿拉伯吡喃糖结合模块。

项目成果

β-L-アラビノピラノシダーゼ

β-L-阿拉伯吡喃糖苷酶

• 发表时间: 2008

A beta-L-arabinopyranosidase from Streptomyces avermitilis is a novel member of glycoside hydrolase family

来自阿维链霉菌的β-L-阿拉伯吡喃糖苷酶是糖苷水解酶家族的新成员

• 发表时间: 2009
• 期刊: J. Biol. Chem 284
• 作者: Hitomi Ichinose;Zui Fujimoto;Mariko Honda;Koichi Harazono;Atsuko Uzura;Satoshi Kaneko
• 通讯作者: Satoshi Kaneko

Structural and functional analysis of beta-L-arabinopyranosidase from Streptomyces avermitilis.

阿维链霉菌β-L-阿拉伯吡喃糖苷酶的结构和功能分析。

• 发表时间: 2009
• 作者: Hitomi Ichinose;Zui Fujimoto;Mariko Honda;Koichi Harazono;Yukifumi Nishimoto;Atsuko Uzura;○Satoshi Kaneko
• 通讯作者: ○Satoshi Kaneko

放線菌のアラビノガラクタン-プロテイン分解酵素

放线菌阿拉伯半乳聚糖蛋白水解酶

• 发表时间: 2008
• 作者: 一ノ瀬仁美;○金子哲
• 通讯作者: ○金子哲

Crystallization and preliminary crystallographic analysis of beta-L-arabinopyranosidase from Streptomyces avermitilis NBRC14893.

来自阿维链霉菌 NBRC14893 的 β-L-阿拉伯吡喃糖苷酶的结晶和初步晶体学分析。

• 发表时间: 2009
• 期刊: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun 65
• 作者: Zui Fujimoto;Hitomi Ichinose;Koichi Harazono;Mariko Honda;Atsuko Uzura;Satoshi Kaneko
• 通讯作者: Satoshi Kaneko