Interferon-producing killer dendritic cell-based immunotherapy for gastrointestinal cancer

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Effective methods for inducing interferon-producing killer dendritic cells (IKDCs) in a mouse model were explored. When murine splenocytes were cultured with CpG, Flt-3 ligand, IL-15, and IL-18, the number of IKDCs was increased to 1.34-6.38 times as much as that in splenocytes without any stimulation. Then, the therapeutic effects of IKDC on the established tumors were assessed using a murine poorly immunogenic colorectal cancer, MC38 model. When IKDCs were inoculated in tumor-bearing mice, the outgrowths of the tumors were significantly suppressed compared with those in mice treated with myeloid dendritic cells. Therefore, IKDC-based immunotherapy should be considered for clinical application.