Developmental Switches: Nuclear Transport and Spermatogenesis

发育开关：核运输和精子发生

• 批准号: DP0346228
• 负责人: Prof Kate Loveland
• 金额: --
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0346228
• 关键词: Developmental; Switches; Nuclear; Transport; Spermatogenesis

We propose to test the novel hypothesis that changes in the nuclear import machinery are a key facet of cellular differentiation. We will investigate a new paradigm in developmental biology regarding whether differentiation is achieved, or can be directed, by altering transport of macromolecules, such as specific transcription factors, into the nucleus. This project will define changes in the nuclear import machinery that accompany germ and somatic cell differentiation in the developing and adult mammalian testis. This will be linked to changes in the function of key proteins acting within the nucleus using both in vitro and in vivo approaches.

我们建议检验这一新的假设，即核进口机制的变化是细胞分化的关键方面。我们将研究发育生物学中的一种新范式，即是否通过改变大分子(如特定的转录因子)进入细胞核的运输来实现或指导分化。这个项目将定义在发育中和成年哺乳动物睾丸中伴随着生殖细胞和体细胞分化的核输入机制的变化。这将与通过体外和体内方法在细胞核内作用的关键蛋白质功能的变化有关。