Examination and pursuit of autologous nerve transplantation using dental pulp

牙髓自体神经移植的检查与探索

• 批准号: 19791477
• 负责人: MATSUSHITA Kazuhiro
• 金额: $ 2.46万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19791477/
• 关键词: 歯髄; 神経移植; 神経再生

We investigated whether dental pulp could be a candidate for graft in a xeno-graft model. It was obtained from the vital extracted tooth for orthodontic treatment, and then treated with freezing and thawing method for killing viable cells and for reducing antigenicity. After putting the dental pulp into chitosan tube, it was transplanted into transected sciatic nerve. Eight weeks after transplantation, regenerated nerve was obviously observed on microscopic investigation, although the mini fascicles were localized and the number of them was small. Anti-neurofilament and Anti-S-100 antibody indicated regeneration as well, suggesting that remaining Schwann cell basal laminae in the pulp acted as a scaffold. Taken together, dental pulp nerve could be a good candidate for nerve grafting in a xeno-graft model.

在异种移植模型中，我们研究了牙髓是否可以作为移植的候选者。取活体拔除的牙齿进行正畸治疗，然后用冻融法杀灭活细胞，降低抗原性。将牙髓置入壳聚糖管内，移植入切断的坐骨神经内。移植后8周，光镜下可见再生神经明显，但微束定位且数量较少。抗神经丝抗体和抗S抗体也提示牙髓再生，提示髓中残留的雪旺细胞基板起支架作用。综上所述，在异种移植模型中，牙髓神经可能是一个很好的神经移植候选者。