Clinical establishment of novel nerve donor as an auto-grafting material. -Application of dental nerve-

新型神经供体作为自体移植材料的临床建立。

• 批准号: 22592251
• 负责人: MATSUSHITA Kazuhiro
• 金额: $ 2.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592251/
• 关键词: 歯髄; 神経移植; 自家移植; 歯髓

This study was designed to examine whether a dental pulp could be a candidate of donor for nerve grafting in xenografting model. The dental pulp was obtained from a human vital extracted tooth, and treated with freezing and thawing method for reducing antigenicity. The treated sample was inserted into chitosan mesh tube, and then the complex was implanted into transected sciatic nerve in Sprague-Dawley (SD) rats. As early as 4 weeks after grafting, regenerating axons accompanied by host Schwann cells were found to grout out through basal laminae by electron microscopy. Twelve weeks after grafting, sporadic axonal regeneration was confirmed by light microscopy, thirty-two weeks after implantation, aggregation of axons was observed in this group. These findings clearly demonstrate that even dental pulp can act as conduits for regenerating axons.

本研究旨在探讨牙髓是否可以作为异种移植模型中神经移植的候选供体。牙髓取自人类活体拔牙，并通过冷冻和解冻方法处理以降低抗原性。将处理后的样品插入壳聚糖网管中，然后将复合物植入 Sprague-Dawley (SD) 大鼠横断的坐骨神经中。早在移植后 4 周，电子显微镜就发现伴随着宿主雪旺细胞的再生轴突通过基底层灌浆出来。移植后12周，光镜下证实有零星的轴突再生，植入后32周，该组观察到轴突聚集。这些发现清楚地表明，甚至牙髓也可以充当轴突再生的管道。

项目成果

An experimental study of xenograft using a hybrid artificial nerve of chitosan nanofiber mesh tube and dental pulp.

壳聚糖纳米纤维网管与牙髓混合人工神经异种移植的实验研究。

• 发表时间: 2012
• 作者: 頭司雄介;他;Kazuhiro Matsushita
• 通讯作者: Kazuhiro Matsushita

An experimental study ofxenograft using a hybrid artificialnerve of chitosan nanofiber mesh tubeand dental pulp

壳聚糖纳米纤维网管与牙髓混合人工神经异种移植的实验研究

• 发表时间: 2012
• 作者: Matsushita K;Wang W;Itoh S;TotsukaYTei K
• 通讯作者: TotsukaYTei K

Dental pulp can be a good candidate for nerve grafting in a xeno-graft model

牙髓可能是异种移植模型中神经移植的良好候选者

• 发表时间: 2012
• 期刊: Journal of Neuroscience Methods
• 影响因子: 3
• 作者: Kazuhiro Matsushita;Wei Wang;Soichiro Itoh;Takanori Domon;Makoto Funahashi ,Yasunori Totsuka
• 通讯作者: Makoto Funahashi ,Yasunori Totsuka

キトサン・ナノ繊維チューブ/歯髄神経ハイブリッド型人工神経を用いた異種移植実験

壳聚糖纳米纤维管/牙髓神经混合人工神经异种移植实验

• 发表时间: 2012
• 作者: 松下和裕;王巍;伊藤聰一郎;柏崎晴彦;戸塚靖則;鄭漢忠
• 通讯作者: 鄭漢忠