Production of useful compounds by means of molecular breeding of oleaginous fungi and unique microbial metabolism

产油真菌高山Mortierella alpina 1S-4产生多种多不饱和脂肪酸，如花生四烯酸。本研究开展了该菌株同源重组的开发、多不饱和脂肪酸相关酶基因的功能分析、多不饱和脂肪酸的分子育种、甾醇生物合成途径的阐明、正构烷烃亚末端氧化机理的研究。这些结果不仅与微生物脂质产生领域有直接联系，而且对阐明微生物脂质代谢机制具有重要意义。

Oleaginous fungus Mortierella alpina 1S-4 produces various polyunsaturated fatty acids such as arachidonic acid. The researcher worked on development of homologous recombination in this strain, functional analysis of enzyme genes involved in polyunsaturated fatty acids, PUFA production through molecular breeding, elucidation of sterol biosynthetic pathways, and study on sub-terminal oxidation mechanism of n-alkanes. The results are considered not only to be directly connected with the field of microbial lipid production but also to be important to elucidate the mechanism of microbial lipid metabolism.