Development of Imaging technique for Alzheimer's disease

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Alzheimer's disease is most common neurodegenerative disorder, which is characterized by progressive memory deficit, cognitive impairment and personal changes. The main pathogenesis is thought extracellular deposits of a short peptide referred to as amyloid β (Aβ), which is major component of the plaque. However, the molecular mechanism for Aβ aggregation process is not well defined. Herein, the aim of this study was to develop new Aβ binding nitroxide to detect fibrils and understand an aggregation process. Aβ binding nitroxide was designed from 6-methoxybenzothiazoleaniline. Fluorescence and ESR spectroscopic analysis demonstrated that the nitroxide banded to Aβ fibrils. Further, we succeeded in detecting of fibrillization process by analyzing the change of ESR spectrum. Another advantage of Aβ binding nitroxide was an antioxidant effect. These findings indicated that new nitroxide can detect Aβ fibrils and evaluate an aggregation process with ESR spectroscopy.