Establishment of the method of A9 dopaminergic neuron differentiation using Lmx1a-GFP homologous recombinant ES cell and transplantation of the GFP+ cells.

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To know the efficacy of A9 dopaminergic (DA) neuron differentiation from mouse ES cell, we established Lmx1a-GFP K/I ES cells by homologous recombination. Depend on the monitoring of GFP expression, we also established the efficient method to induce DA neurons from ES cells with appropriate concentration of noggin, Shh, and FGF8 factors. After transplantation of GFP positive cells, they survived and differentiated into A9 DA neurons in the striatum of Parkinsonian mice.