Production of the safer induced pluripotent stem cells and their application for regenerative medicine

诱导多能干细胞(IPS)由逆转录病毒载体、慢病毒载体和腺病毒载体三种转导系统制备。慢病毒载体的生产效率最高。这是因为慢病毒载体可以表现出最高的转导效率。与传统的诱导分化方法相比，携带CA启动子的腺病毒载体转导HOXB4基因、PPARγ基因和Runx2基因后，造血祖细胞、脂肪细胞和成骨细胞分别得到有效的诱导。这些结果表明，腺病毒载体可能是一种有效地向iPS细胞分化的有用工具。

Induced pluripotent stem (iPS) cells were produced by threetransduction systems, retroviral vectors, lentiviral vectors, and adenoviral vectors. The highest production efficiency was obtained when lentiviral vectors were used. This is because lentiviral vectors could show the highest transduction efficiency. When HoxB4 gene, PPARγ gene, and Runx2 gene were transduced with adenoviral vectors with the CA promoter, hematopoietic progenitor cells, adipocytes, and osteoblasts were efficiently induced, respectively, in comparison with conventional differentiation methods. These results demonstrate that adenoviral vectors could be a useful tool for efficient differentiation from iPS cells.