Expression Analysis of the Exchangeable Gene Trap Clones by X-gal Staining

我们用X-gal染色法研究EGTC小鼠细胞系的表达谱。我们分析了84个小鼠系，并打开了67个克隆的表达模式。在许多情况下，多个器官表现出强烈的β -geo蛋白表达。X-gal染色结果与EST谱（Unigene）的TPM（每百万转录本）分数几乎没有关系。这一发现表明，当我们将报告基因作为产生转基因小鼠的启动子资源时，实际监测其表达模式的重要性。

We investigated the expression pattern of the established EGTC mouse lines by X-gal staining. We have analyzed 84 mouse lines, and opened expression patterns of 67 clones. In many case, multiple organs showed strong expression of beta-geo protein. There are almost no relationship between X-gal staining results and TPM (transcripts per million) scores of the EST profile (Unigene). This finding shows the importance of actually monitoring the expression pattern of the reporter gene, when we consider it as promoter resources to produce transgenic mice.