Establishment of Cre-driver mouse lines by using the Exchangeable Gene Trap Ckones.

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Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, most protein coding genes are producing conditional knock out allele. However a variety of Cre driver mouse line, which has an important role about conditional knock out system, is not enough.We have developed Database for the Exchangeable Gene Trap Clones; EGTC (http://egtc.jp). In our system, reporter beta-geo gene can be exchanged into any other DNA of interest through Cre-mediated recombination.We have produced more than 300 Cre driver ES cell lines using EGTC ES cells. Among them, about 250 clones were provided to make chimeric mice, and more than 100 mouse lines could be established. Then we did mating of Cre driver mice with ROSA26R mice, and performed X-gal staining of ROSA26R mice with Cre driver allele. Among them, 21-T188SACre mouse line exhibited the cerebellum specific expression pattern.