Development of fundamental technology for accurate RNAomics

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Fundamental technology including separation and mass spectrometry for miRNA measurement have been studied. Micro flow meter based separation system was optimized. LC system was established through optimization of elutant, matrix, column etc. De novo sequencing of miRNA (longer than 20 base) was achieved by means of iontrap-time of flight mass spectrometry based MS(N) analysis. Ion mobility based mass spectrometry was also performed to separate sequence isomers. In addition, RNA analogous MS probe was created.