Determination of functional sequences available for horticulture in viroid nucleotide

类病毒核苷酸中可用于园艺的功能序列的测定

• 批准号: 20380020
• 负责人: HOSOKAWA Munetaka
• 金额: $ 12.48万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380020/
• 关键词: ウイロイド; キク; 機能性RNA; プロモーター; 長距離移行性; キクわい化ウイロイド(CSVd); ポスピウイロイド; 長距離移行; タバコ; アラビドプシス; プロモーター活性; キクわい化ウイロイド; 開花反応; 局在性; CSVd; CChMVd; 核

The full length of CSVd or its partial sequence was connected in additional sequences, the long-distance transmissibility of additional sequences were examined. Since the additional sequences which were added with the sequence of a partial CSVd had a long-distance transferability, it was presumed that there is a capability in the sequence of CSVd for transferring the RNA for a long distance.In order to confirm this fact, a tobacco recombinant which excessively develops similar sequences was created regarding the partial sequence series of CSVd. Although a section of Nicotiana benthamiana was grafted in the tobacco recombinant (Nicotiana tabacum) with CSVd of full-length sequences and the long-distance transferability of addition sequences was examined, a long-distance transferability was not found. The examinations of other sequences are still ongoing.Moreover, it was indicated that CSVd sequences have a function for reinforcing 35S promoter's activity. Furthermore, since the ability for transcribing RNA using the template of mRNA which CSVd is expected to have was confirmed in the recombinant tobacco, it is thought that the mRNA multiplication in a plant is possible.

将CSVd全长或其部分序列连接成附加序列，检测附加序列的远距离传递性。由于添加了部分CSVd序列的附加序列具有长距离转移性，因此推测CSVd序列具有长距离转移RNA的能力。将本氏烟草（Nicotiana benthamiana）的一段CSVd全长序列嫁接到烟草重组体（Nicotiana tabacum）中，检测了CSVd全长序列的远距离转移性，未发现CSVd全长序列的远距离转移性，其他序列的检测仍在进行中，并表明CSVd全长序列具有增强35S启动子活性的功能。此外，由于在重组烟草中确认了使用CSVd预期具有的mRNA模板转录RNA的能力，因此认为在植物中的mRNA增殖是可能的。

项目成果

In Vitro-Transcribed Chrysanthemum stunt viroid RNA Is Infectious to Chrysanthemum and Other Plants

• DOI: 10.1094/phyto-99-1-0058
• 发表时间: 2009-01-01
• 期刊: PHYTOPATHOLOGY
• 影响因子: 3.2
• 作者: Matsushita, Yosuke;Penmetcha, Kumar K. R.
• 通讯作者: Penmetcha, Kumar K. R.

新しいウイロイドフリーの方法としての超微小茎頂分裂組織培養法(仮題)

超小芽顶端分生组织培养方法作为一种新的无类病毒方法（暂定名称）

• 发表时间: 2011
• 期刊: 植物防疫 (掲載確定)
• 作者: 林裕作;河村耕史;白武勝裕;松本省吾;細川宗孝
• 通讯作者: 細川宗孝

ウイロイドフリー植物作出のための新手法・超微小茎頂分裂組織培養法

生产无类病毒植物的新方法：超小芽顶端分生组织培养法

• 发表时间: 2011
• 期刊: 植物防疫
• 作者: Ueno;O.;細川宗孝
• 通讯作者: 細川宗孝

キクわい化ウイロイドに抵抗性を持つキク品種の探索

寻找抗菊花矮化类病毒的菊花品种

• 发表时间: 2011
• 作者: 廖易;庄得鳳;鈴木健太;高井康弘;瀬川絵理子;于文進;嶋津光鑑;河本英二;福井博一;上野修;細川宗孝・鍋島朋之・矢野志野布・大石一史・土井元章
• 通讯作者: 細川宗孝・鍋島朋之・矢野志野布・大石一史・土井元章