The fumctional siRAs inducing flowering of chrysanthemum plants

诱导菊花开花的功能性 siRA

• 批准号: 17380022
• 负责人: HOSOKAWA Munetaka
• 金额: $ 10.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380022/
• 关键词: CSVd; Transgenic plants; Functional RNA; siRNA; Chrysanthemum; Photoperiodism; Viroid; Gene silencing; キクスタントウイロイド; 超微小茎頂分裂組織培養法; ウイロイドフリー; CSVd抵抗性; Multiplex RT-PCR; 生体内濃度

Chrysanthemum is one of the obligative short-day flowering plants. Therefore, usually the flowering of chrysanthemum can be controlled by photoperiod. However, it was noted that 'Piato' plants infected by the CSVd flowered autonomously even under long-day conditions. In this study, we clarified the relationship between CSVd infection and the autonomous flowering of 'Piato' plants regenerated from different-sized dissected shoot apical meristems. Under natural short-day conditions, the flowering of plants regenerated from SAMs containing LPs was one month earlier than plants regenerated from LP-free SAMs. CSVd was detected from these early flowering plants by RT-PCR. On the other hand, CSVd was not detected in plants regenerated from LP-free SAMs. CSVd-infected and -free plants were grown under long-day conditions simulated by night-break lighting at 22: 00-2: 00. All CSVd-infected plants flowered autonomously even under long-day conditions; on the other hand, CSVd-free chrysanthemum pl … More ants maintained their vegetative growth. When the CSVd-free plants were inoculated with CSVd by grafting them to CSVd-infected root stocks, they flowered autonomously even under night-break lighting. In this study, results suggest that CSVd may control the qualitative development process, flowering; that is, it can induce the autonomous flowering of chrysanthemum.We introduced siRNA expressed vectors to 'I-69-8' plants and produced several transgenic plants. One line introduced by vector1 which has sense and antisence strand of 120bp CSVd sequences and two lines introduced by vector2 having 120bp CSVd sense sequence express a little bit of resistance to CSVd. And time course observation clarified that in these lines CSVd titers increase CSVd after inoculation and then decreases gradually. And one line introduced by vector1 was only partly infected by CSVd in a leaf. So these plants are supposed to have a siRNA produced by the break down by the interaction between infected CSVd molecules and introduced genes. I must check siRNA production in plants and the flowering time of these plants. Less

菊花是必不可少的短期开花植物之一。因此，通常可以通过光周期控制菊花的开花。但是，人们指出的是，即使在长期的条件下，也会自主开花的“ Piato”植物自动开花。在这项研究中，我们阐明了CSVD感染与从不同尺寸的解剖芽顶分生组织中再生的“ Piato”植物的自主开花之间的关系。在天然短期条件下，从含有LPS的SAM再生的植物开花比从无LP的SAM再生的植物早一个月。 RT-PCR从这些早期开花植物中检测到CSVD。另一方面，在不含LP的Sams再生的植物中未检测到CSVD。在夜间夜间照明的夜间模拟22：00-2：00的长时间模拟的情况下，感染了CSVD的植物和 - 植物。另一方面，不含CSVD的菊花pl…更多的蚂蚁保持了营养生长。当无CSVD植物通过将CSVD接种到CSVD感染的根库存中时，即使在夜间夜间照明下，它们也会自动开花。在这项研究中，结果表明CSVD可以控制定性发展过程，开花。也就是说，它可以诱导菊花的自主开花。我们引入了siRNA表达的矢量为“ I-69-8”植物，并生产了几种转基因植物。 Vector1引入的一条线，具有120bp CSVD序列的感官和反式链，而Vector2引入的两条线具有120BP CSVD sense sequence sequence sexpers表达对CSVD的抗性。时间课程观察表明，在这些线路中，CSVD滴度在接种后增加CSVD，然后逐渐减少。 Vector1引入的一条线仅部分被叶片中的CSVD感染。因此，这些植物有望通过受感染的CSVD分子与引入基因之间的相互作用而产生的siRNA。我必须检查植物中的siRNA生产和这些植物的开花时间。较少的

项目成果

Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue

• DOI: 10.1016/j.jviromet.2005.07.001
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF VIROLOGICAL METHODS
• 影响因子: 3.1
• 作者: Hosokawa, M;Matsushita, Y;Yazawa, S
• 通讯作者: Yazawa, S

Screening of a chrysanthemum cultivar with some mechanism of suppressing concentration-increase of Chrysanthemum stunt viroid

具有抑制菊花矮化类病毒浓度升高作用的菊花品种的筛选

• 发表时间: 2006
• 作者: M.;Hosokawa;H.;Shiba;S.;Yazawa
• 通讯作者: Yazawa

Screening of a chrysanthemum plant having strong resistance for Chrysanthemum stunt viroid

对菊花矮化类病毒具有强抗性的菊花植株的筛选

• 发表时间: 2008
• 作者: M.;Hosokawa;H.;Omori;S.;Yazawa
• 通讯作者: Yazawa

Identification and Sterilization of Epiphytic Bacterial Flora Existing near Hydrangea Shoot A

绣球A芽附近附生细菌菌群的鉴定与灭菌

• 发表时间: 2008
• 期刊: J. Jpn Soc. Hort. Sci. (in press)
• 作者: Y. Kitamura;M. Hosokawa;C. Tanaka,
• 通讯作者: C. Tanaka,

キクわい化ウイロイドに強い抵抗性を持つキク植物体の選抜

抗菊花矮化类病毒的菊花植株选育

• 发表时间: 2008
• 期刊: 園芸学研究 7
• 作者: Watari;A.;T. Hanada;H. Yamane;T. Esumi;R. Tao;H. Yaegaki;M. Yamaguchi;K. Beppu;and I. Kataoka.;細川宗孝・尾森仁美・矢澤進
• 通讯作者: 細川宗孝・尾森仁美・矢澤進