The fumctional siRAs inducing flowering of chrysanthemum plants

诱导菊花开花的功能性 siRA

• 批准号: 17380022
• 负责人: HOSOKAWA Munetaka
• 金额: $ 10.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380022/
• 关键词: CSVd; Transgenic plants; Functional RNA; siRNA; Chrysanthemum; Photoperiodism; Viroid; Gene silencing; キクスタントウイロイド; 超微小茎頂分裂組織培養法; ウイロイドフリー; CSVd抵抗性; Multiplex RT-PCR; 生体内濃度

Chrysanthemum is one of the obligative short-day flowering plants. Therefore, usually the flowering of chrysanthemum can be controlled by photoperiod. However, it was noted that 'Piato' plants infected by the CSVd flowered autonomously even under long-day conditions. In this study, we clarified the relationship between CSVd infection and the autonomous flowering of 'Piato' plants regenerated from different-sized dissected shoot apical meristems. Under natural short-day conditions, the flowering of plants regenerated from SAMs containing LPs was one month earlier than plants regenerated from LP-free SAMs. CSVd was detected from these early flowering plants by RT-PCR. On the other hand, CSVd was not detected in plants regenerated from LP-free SAMs. CSVd-infected and -free plants were grown under long-day conditions simulated by night-break lighting at 22: 00-2: 00. All CSVd-infected plants flowered autonomously even under long-day conditions; on the other hand, CSVd-free chrysanthemum pl … More ants maintained their vegetative growth. When the CSVd-free plants were inoculated with CSVd by grafting them to CSVd-infected root stocks, they flowered autonomously even under night-break lighting. In this study, results suggest that CSVd may control the qualitative development process, flowering; that is, it can induce the autonomous flowering of chrysanthemum.We introduced siRNA expressed vectors to 'I-69-8' plants and produced several transgenic plants. One line introduced by vector1 which has sense and antisence strand of 120bp CSVd sequences and two lines introduced by vector2 having 120bp CSVd sense sequence express a little bit of resistance to CSVd. And time course observation clarified that in these lines CSVd titers increase CSVd after inoculation and then decreases gradually. And one line introduced by vector1 was only partly infected by CSVd in a leaf. So these plants are supposed to have a siRNA produced by the break down by the interaction between infected CSVd molecules and introduced genes. I must check siRNA production in plants and the flowering time of these plants. Less

菊花是专性短日照开花植物之一。因此，通常菊花的开花可以通过光周期来控制。然而，注意到被CSVd感染的“Piato”植物即使在长日照条件下也能自主开花。在这项研究中，我们澄清了CSVd感染和自主开花的'Piato'植物再生从不同大小的解剖茎顶端分生组织之间的关系。在自然短日照条件下，含脂多糖的自组装膜再生植株的开花时间比不含脂多糖的自组装膜再生植株提前一个月。用RT-PCR方法从这些早花植物中检测到CSVd。另一方面，在从无LP的自组装膜再生的植物中未检测到CSVd。在22：00-2：00的夜间照明模拟的长日照条件下生长CSVD感染的和无CSVD的植物。所有感染CSVd的植株即使在长日照条件下也能自主开花;另一方面，不感染CSVd的菊花植株在长日照条件下也能自主开花。 ...更多信息 蚂蚁维持着营养生长。当CSVd无植物接种CSVd，将它们嫁接到CSVd感染的砧木，他们开花自主，即使在夜间休息照明。本研究的结果表明，CSVd基因可能控制菊花开花的质量发育过程，即诱导菊花的自主开花。我们将siRNA表达载体导入到'I-69-8'植株中，获得了几株转基因植株。载体1导入的1个株系含有120 bp的CSVd正义链和反义链，载体2导入的2个株系含有120 bp的CSVd正义链，对CSVd有一定的抗性。通过时程观察，发现在这些品系中，接种后CSVd滴度增加，然后逐渐下降。其中1个株系的叶片仅部分感染CSVd。因此，这些植物应该有一个siRNA产生的破坏之间的相互作用感染CSVd分子和引入的基因。我必须检查植物中siRNA的产生和这些植物的开花时间。少

项目成果

Screening of a chrysanthemum cultivar with some mechanism of suppressing concentration-increase of Chrysanthemum stunt viroid

具有抑制菊花矮化类病毒浓度升高作用的菊花品种的筛选

• 发表时间: 2006
• 作者: M.;Hosokawa;H.;Shiba;S.;Yazawa
• 通讯作者: Yazawa

Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue

• DOI: 10.1016/j.jviromet.2005.07.001
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF VIROLOGICAL METHODS
• 影响因子: 3.1
• 作者: Hosokawa, M;Matsushita, Y;Yazawa, S
• 通讯作者: Yazawa, S

Identification and Sterilization of Epiphytic Bacterial Flora Existing near Hydrangea Shoot A

绣球A芽附近附生细菌菌群的鉴定与灭菌

• 发表时间: 2008
• 期刊: J. Jpn Soc. Hort. Sci. (in press)
• 作者: Y. Kitamura;M. Hosokawa;C. Tanaka,
• 通讯作者: C. Tanaka,

キクわい化ウイロイドに強い抵抗性を持つキク植物体の選抜

抗菊花矮化类病毒的菊花植株选育

• 发表时间: 2008
• 期刊: 園芸学研究 7
• 作者: Watari;A.;T. Hanada;H. Yamane;T. Esumi;R. Tao;H. Yaegaki;M. Yamaguchi;K. Beppu;and I. Kataoka.;細川宗孝・尾森仁美・矢澤進
• 通讯作者: 細川宗孝・尾森仁美・矢澤進

Screening of a chrysanthemum plant having strong resistance for Chrysanthemum stunt viroid

对菊花矮化类病毒具有强抗性的菊花植株的筛选

• 发表时间: 2008
• 作者: M.;Hosokawa;H.;Omori;S.;Yazawa
• 通讯作者: Yazawa