The fumctional siRAs inducing flowering of chrysanthemum plants

诱导菊花开花的功能性 siRA

• 批准号: 17380022
• 负责人: HOSOKAWA Munetaka
• 金额: $ 10.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380022/
• 关键词: CSVd; Transgenic plants; Functional RNA; siRNA; Chrysanthemum; Photoperiodism; Viroid; Gene silencing; キクスタントウイロイド; 超微小茎頂分裂組織培養法; ウイロイドフリー; CSVd抵抗性; Multiplex RT-PCR; 生体内濃度

Chrysanthemum is one of the obligative short-day flowering plants. Therefore, usually the flowering of chrysanthemum can be controlled by photoperiod. However, it was noted that 'Piato' plants infected by the CSVd flowered autonomously even under long-day conditions. In this study, we clarified the relationship between CSVd infection and the autonomous flowering of 'Piato' plants regenerated from different-sized dissected shoot apical meristems. Under natural short-day conditions, the flowering of plants regenerated from SAMs containing LPs was one month earlier than plants regenerated from LP-free SAMs. CSVd was detected from these early flowering plants by RT-PCR. On the other hand, CSVd was not detected in plants regenerated from LP-free SAMs. CSVd-infected and -free plants were grown under long-day conditions simulated by night-break lighting at 22: 00-2: 00. All CSVd-infected plants flowered autonomously even under long-day conditions; on the other hand, CSVd-free chrysanthemum pl … More ants maintained their vegetative growth. When the CSVd-free plants were inoculated with CSVd by grafting them to CSVd-infected root stocks, they flowered autonomously even under night-break lighting. In this study, results suggest that CSVd may control the qualitative development process, flowering; that is, it can induce the autonomous flowering of chrysanthemum.We introduced siRNA expressed vectors to 'I-69-8' plants and produced several transgenic plants. One line introduced by vector1 which has sense and antisence strand of 120bp CSVd sequences and two lines introduced by vector2 having 120bp CSVd sense sequence express a little bit of resistance to CSVd. And time course observation clarified that in these lines CSVd titers increase CSVd after inoculation and then decreases gradually. And one line introduced by vector1 was only partly infected by CSVd in a leaf. So these plants are supposed to have a siRNA produced by the break down by the interaction between infected CSVd molecules and introduced genes. I must check siRNA production in plants and the flowering time of these plants. Less

菊花是专性短日照开花植物之一。因此，通常菊花的开花可以通过光周期来控制。然而，人们注意到，被 CSVd 感染的“Piato”植物即使在长日照条件下也能自主开花。在这项研究中，我们阐明了 CSVd 感染与从不同大小的解剖茎尖分生组织再生的“Piato”植物自主开花之间的关系。在自然短日照条件下，含有LPs的SAM再生的植物比不含LPs的SAM再生的植物早1个月开花。通过 RT-PCR 从这些早期开花植物中检测到 CSVd。另一方面，在从不含 LP 的 SAM 再生的植物中未检测到 CSVd。感染和未感染 CSVd 的植物在 22: 00-2: 00 夜间照明模拟的长日照条件下生长。所有感染 CSVd 的植物即使在长日照条件下也能自主开花；另一方面，不含CSVd的菊花蚂蚁则保持了营养生长。当通过将不含 CSVd 的植物嫁接到 CSVd 感染的根茎上来接种 CSVd 时，它们即使在夜间照明下也能自主开花。在这项研究中，结果表明 CSVd 可能控制定性发育过程、开花；也就是说，它可以诱导菊花自主开花。我们将siRNA表达载体引入'I-69-8'植物中，并产生了几种转基因植物。由具有120bp CSVd序列的有义链和反义链的载体1引入的一个品系和由具有120bp CSVd有义序列的载体2引入的两个品系对CSVd表现出一点抗性。时程观察表明，在这些品系中，接种后 CSVd 滴度增加，然后逐渐降低。而由 vector1 引入的一个品系仅部分被叶子中的 CSVd 感染。因此，这些植物应该具有由受感染的 CSVd 分子和引入基因之间相互作用分解产生的 siRNA。我必须检查植物中 siRNA 的产生以及这些植物的开花时间。较少的

项目成果

Screening of a chrysanthemum cultivar with some mechanism of suppressing concentration-increase of Chrysanthemum stunt viroid

具有抑制菊花矮化类病毒浓度升高作用的菊花品种的筛选

• 发表时间: 2006
• 作者: M.;Hosokawa;H.;Shiba;S.;Yazawa
• 通讯作者: Yazawa

Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue

• DOI: 10.1016/j.jviromet.2005.07.001
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF VIROLOGICAL METHODS
• 影响因子: 3.1
• 作者: Hosokawa, M;Matsushita, Y;Yazawa, S
• 通讯作者: Yazawa, S

Identification and Sterilization of Epiphytic Bacterial Flora Existing near Hydrangea Shoot A

绣球A芽附近附生细菌菌群的鉴定与灭菌

• 发表时间: 2008
• 期刊: J. Jpn Soc. Hort. Sci. (in press)
• 作者: Y. Kitamura;M. Hosokawa;C. Tanaka,
• 通讯作者: C. Tanaka,

キクわい化ウイロイドに強い抵抗性を持つキク植物体の選抜

抗菊花矮化类病毒的菊花植株选育

• 发表时间: 2008
• 期刊: 園芸学研究 7
• 作者: Watari;A.;T. Hanada;H. Yamane;T. Esumi;R. Tao;H. Yaegaki;M. Yamaguchi;K. Beppu;and I. Kataoka.;細川宗孝・尾森仁美・矢澤進
• 通讯作者: 細川宗孝・尾森仁美・矢澤進

Screening of a chrysanthemum plant having strong resistance for Chrysanthemum stunt viroid

对菊花矮化类病毒具有强抗性的菊花植株的筛选

• 发表时间: 2008
• 作者: M.;Hosokawa;H.;Omori;S.;Yazawa
• 通讯作者: Yazawa