25℃ gene switch: Solanaceae plants in tropical area, which control their growth and development responding to small temperature difference

25℃基因开关：热带茄科植物，响应微小温差控制其生长发育

• 批准号: 22405017
• 负责人: HOSOKAWA Munetaka
• 金额: $ 11.56万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22405017/
• 关键词: 蔬菜; トウガラシ; 恒温性地域; ウイルス; 縮葉; Capsicum chinense; インドネシア; ジェミニウイルス; 遺伝資源; 温度反応性; 遺伝子発現; 耐病性関連遺伝子; サリチル酸; 形態

When ‘Sy-2’ (Capsicum chinense) plants are grown under 24℃, the abnormal morphology in their leaves are observed. The trigger for the abnormality seemed to be salicylic acid induction in the plants and the compound induces the active expression of pathogen-related proteins. On the basis of the hypothesis that the growth of ‘Sy-2’ at areas with a constant temperature (24-26℃) will induces pathogen, such as such as viruses, resistance, we cultivated ‘Sy-2’ in Indonesia, tropical country with constanttemperature. On the contrary of the hypothesis, ‘Sy-2’ plants were infected with viruses like control cultivars. However, in some traditional cultivars and ‘Sy-2’ grown in Indonesia, shoots generation from virus infected plants were observed. Longer-time observation should be needed for the evaluation of temperature sensing for pathogen resistance of ‘Sy-2’. In this project, we also collected many cultivars from Indonesia and Caribbean community and found a capsicum cultivar (Capsicum chinense) with the interesting phenotype for temperature response like ‘Sy-2’. The genetic mechanisms for the morphology of the cultivar seemed to be similar to ‘Sy-2’. We should analyze the interesting phenotype in detail and consider how to use the bio-resources in tropical area.

当“Sy-2”（辣椒）植株在24℃下生长时，观察到其叶片形态异常。异常的触发因素似乎是植物中的水杨酸诱导，并且该化合物诱导病原体相关蛋白的活跃表达。基于“Sy-2”在恒温（24-26℃）地区生长会诱导病毒等病原体产生抵抗力的假设，我们在恒温热带国家印度尼西亚培育了“Sy-2”。与假设相反，‘Sy-2’植物像对照品种一样被病毒感染。然而，在印度尼西亚种植的一些传统品种和“Sy-2”中，观察到病毒感染植物产生芽。 ‘Sy-2’的温度传感对病原菌抗性的评估需要更长时间的观察。在这个项目中，我们还收集了来自印度尼西亚和加勒比社区的许多品种，并发现了一个具有有趣的温度响应表型的辣椒品种（Capsicum chinense），如“Sy-2”。该品种形态的遗传机制似乎与‘Sy-2’相似。我们应该详细分析感兴趣的表型并考虑如何利用热带地区的生物资源。

项目成果

自然変異によるトウガラシの生育温度域での温度反応性形質は恒温性地域において潜在化する

由于自然变异而导致的辣椒生长温度范围内的温度响应性状在恒温地区变得潜在

• 发表时间: 2012
• 作者: 小枝壮太;細川宗孝;ら
• 通讯作者: ら

Characterization and genetic analysis of a low-temperature-sensitive mutant, sy-2, in Capsicum chinense

• DOI: 10.1007/s00122-010-1460-0
• 发表时间: 2011-02-01
• 期刊: THEORETICAL AND APPLIED GENETICS
• 影响因子: 5.4
• 作者: An, Song-Ji;Pandeya, Devendra;Kang, Byoung-Cheorl
• 通讯作者: Kang, Byoung-Cheorl

emperature-sensitive phenotype caused by natural mutation in Capsicum latescent in two tropical regions.

两个热带地区晚熟辣椒自然突变引起的温度敏感表型。

• 发表时间: 2013
• 期刊: Journal of Plant Research
• 影响因子: 2.8
• 作者: Tajima;R. and Y.Kato;Sota Koeda
• 通讯作者: Sota Koeda

Developmental abnormality of a local pepper cultivar (Capsicum chinense) from the Seychelles below 24℃

塞舌尔地方辣椒品种Capsicum chinense在24℃以下发育异常

• 发表时间: 2010
• 期刊: Bulletin of Experimental Farm Kyoto University
• 作者: Sota Koeda;Akane Takezaki;Yuki Isomura;Susumu Yazawa
• 通讯作者: Susumu Yazawa

非辛味トウガラシNo.3341（Capsicum chinense)におけるcaffeic acid O-methyltransferase（Comt）の変異.

非刺激性辣椒 3341 号（辣椒）中咖啡酸 O-甲基转移酶 (Comt) 的突变。

• 发表时间: 2013
• 作者: Kaneko I;Iyama-Kadono M;Togashi-Nishigata K;Yamaguchi I;Teraoka T;Arie T;小枝壮太
• 通讯作者: 小枝壮太